format-version: 1.2 data-version: releases/2016-11-04 date: 10:05:2016 15:33 saved-by: snadendla auto-generated-by: OBO-Edit 2.3.1 subsetdef: go_groupings "Grouping classes used by GO" subsetdef: valid_with_biological_process "GO biological process terms should be used in the GO with/from field" subsetdef: valid_with_cellular_component "GO cellular component terms should be used in the GO with/from field" subsetdef: valid_with_chemical_entity "Chemical entity IDs should be used in the GO with/from field" subsetdef: valid_with_gene "Gene IDs should be used in the GO with/from field" subsetdef: valid_with_molecular_function "GO molecular function terms should be used in the GO with/from field" subsetdef: valid_with_protein "Protein IDs should be used in the GO with/from field" subsetdef: valid_with_protein_complex "Protein complex IDs should be used in the GO with/from field" subsetdef: valid_with_transcript "Transcript IDs should be used in the GO with/from field" default-namespace: eco remark: For more details, see https://github.com/evidenceontology/evidenceontology ontology: eco [Term] id: ECO:0000000 name: evidence def: "A type of information that is used to support an assertion." [ECO:MCC] synonym: "evidence code" RELATED [] synonym: "evidence_code" RELATED [] disjoint_from: ECO:0000217 ! assertion method [Term] id: ECO:0000001 name: inference from background scientific knowledge def: "A curator inference that links the current annotation to a different evidence-based annotation via background knowledge of the curator." [ECO:go, ECO:MCC] is_a: ECO:0000205 ! curator inference [Term] id: ECO:0000002 name: direct assay evidence def: "Experimental evidence that is based on direct measurement of some aspect of a biological feature." [ECO:MCC] is_a: ECO:0000006 ! experimental evidence [Term] id: ECO:0000003 name: reconstitution assay evidence def: "A type of direct assay evidence based on reconstructing a biological sample from its disassociated state to its original state." [ECO:KAV, PMID:26029343] is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000004 name: cell fractionation evidence def: "A type of fractionation evidence where sub-cellular components are separated based on their physical properties such as density in a sucrose density gradient." [ECO:KIM, TAIR:TED] comment: If using this term for Gene Ontology annotation, it would be used most typically for annotations to the cellular component ontology. synonym: "IDA: cell fractionation" RELATED [] is_a: ECO:0000100 ! fractionation evidence [Term] id: ECO:0000005 name: enzyme assay evidence def: "Used when an annotation is made based on assays that determine the catalytic activity of enzymes." [TAIR:TED] synonym: "IDA: enzyme assays" RELATED [] xref: PSI-MI:MI\:0415 "enzymatic study" is_a: ECO:0000039 ! protein assay evidence [Term] id: ECO:0000006 name: experimental evidence def: "An evidence type that is based on the results of a laboratory assay." [ECO:MCC, GOC:ecd] subset: go_groupings is_a: ECO:0000000 ! evidence [Term] id: ECO:0000007 name: immunofluorescence evidence def: "A type of protein detection assay evidence where a fluorescently labeled antibody is used to detect the presence or localization of a biomolecule within a cell." [ECO:MCC, TAIR:TED] synonym: "IDA: immunofluorescence" RELATED [] is_a: ECO:0005517 ! protein detection assay evidence [Term] id: ECO:0000008 name: expression pattern evidence def: "A type of experimental evidence that is based on characterization of gene expression." [ECO:MCC, GO:IEP] comment: Use this evidence type when the annotation is inferred from the timing or location of expression of a gene. It may be difficult to determine whether the expression pattern truly indicates that a gene plays a role in a given process. is_a: ECO:0000006 ! experimental evidence [Term] id: ECO:0000009 name: transcript expression evidence is_a: ECO:0000008 ! expression pattern evidence [Term] id: ECO:0000010 name: protein expression evidence is_a: ECO:0000008 ! expression pattern evidence is_a: ECO:0000039 ! protein assay evidence [Term] id: ECO:0000011 name: genetic interaction evidence xref: TAIR:TED\:0000006 is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000012 name: functional complementation evidence def: "Used when an annotation is made based on a functional complementation assay in which a wild-type copy of the gene in question is inserted into a mutant background in the organism of the gene's origin or a heterologous organism with a mutation in the homologous gene." [TAIR:TED] synonym: "IGI: functional complementation" RELATED [] is_a: ECO:0000011 ! genetic interaction evidence [Term] id: ECO:0000013 name: transgenic rescue experiment evidence is_a: ECO:0000012 ! functional complementation evidence [Term] id: ECO:0000015 name: mutant phenotype evidence def: "The IMP evidence code covers those cases when the function, process or cellular localization of a gene product is inferred based on differences in the function, process, or cellular localization between two different alleles of the corresponding gene. The IMP code is used for cases where one allele may be designated 'wild-type' and another as 'mutant'. It is also used in cases where allelic variation occurs naturally and no specific allele is designated as wild-type or mutant. Caution should be used when making annotations from gain-of-function mutations as it may be difficult to infer a gene's normal function from a gain of function mutation, although it is sometimes possible." [GO:IMP] is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000016 name: loss-of-function mutant phenotype evidence is_a: ECO:0000015 ! mutant phenotype evidence [Term] id: ECO:0000017 name: ectopic expression evidence def: "Used when an annotation is made based on the analysis of the phenotype of a wild-type or mutant transgenic organism that has been engineered to overexpress or ectopically express the gene product in question." [TAIR:TED] synonym: "IMP: analysis of overexpression/ectopic expression phenotype" RELATED [] is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000018 name: anti-sense experiment evidence def: "Used when an annotation is based on a phenotype observed when expressing an anti-sense version of a gene product in a wild-type (for that gene product) background." [TAIR:TED] synonym: "IMP: anti-sense experiments" RELATED [] is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000019 name: RNAi evidence def: "Inference based on a phenotype observed when expressing an RNAi construct that includes a fragment of the gene product in a wild-type (for that gene product) background. A double-stranded RNA specifically suppresses the expression of the gene bearing its complementary sequence." [TAIR:TED] synonym: "IMP: RNAi experiment" RELATED [] is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000020 name: specific protein inhibition evidence def: "A type of protein assay evidence based on the inhibition of the molecular function of a protein." [ECO:MCC] is_a: ECO:0000039 ! protein assay evidence [Term] id: ECO:0000021 name: physical interaction evidence def: "Experimental evidence that is based on characterization of an interaction between a gene product and another molecule." [ECO:MCC] comment: Molecules interacted with might include protein, nucleic acid, ion, or complex. xref: PSI-MI:MI\:0045 "experimental interaction detection" is_a: ECO:0000006 ! experimental evidence [Term] id: ECO:0000022 name: co-purification evidence def: "A type of physical interaction evidence where a cellular component subunit is isolated as part of purification of its larger complex." [TAIR:TED] synonym: "IPI: co-purification" RELATED [] xref: PSI-MI:MI\:0025 "copurification" is_a: ECO:0000021 ! physical interaction evidence [Term] id: ECO:0000023 name: affinity evidence def: "A type of physical interaction evidence that depends on the strength of the interaction between two entities." [ECO:MCC, PSI-MI:MI\:0400] synonym: "ligand binding evidence" NARROW [] xref: PSI-MI:MI\:0400 "affinity technology" is_a: ECO:0000021 ! physical interaction evidence [Term] id: ECO:0000024 name: protein binding evidence is_a: ECO:0000023 ! affinity evidence is_a: ECO:0000039 ! protein assay evidence [Term] id: ECO:0000025 name: hybrid interaction evidence def: "A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC, PSI-MI:MI\:0090] comment: Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode. xref: PSI-MI:MI\:0090 "protein complementation assay" is_a: ECO:0000023 ! affinity evidence [Term] id: ECO:0000026 name: nucleic acid hybridization evidence is_a: ECO:0000073 ! experimental genomic evidence [Term] id: ECO:0000027 name: structural similarity evidence def: "A type of similarity evidence based on structural similarity of an annotated gene or gene product to another gene or group of genes." [ECO:MCC, TAIR:TED] comment: For GO annotation, in the case of a single gene, an accession for the related gene's sequence is entered in the evidence_with field. is_a: ECO:0000057 ! phenotypic similarity evidence [Term] id: ECO:0000028 name: motif similarity evidence def: "Used when an annotation is made based on the presence of a recognized domain or motif in a gene product's (usually protein) primary sequence." [TAIR:TED] synonym: "ISS: recognized domains" RELATED [] is_a: ECO:0000202 ! match to sequence model evidence [Term] id: ECO:0000029 name: match to InterPro member signature evidence is_a: ECO:0000202 ! match to sequence model evidence [Term] id: ECO:0000030 name: BLAST evidence used in manual assertion def: "A type of BLAST evidence that is used in a manual assertion." [ECO:MCC] synonym: "curated BLAST analysis" RELATED [] is_a: ECO:0000206 ! BLAST evidence is_a: ECO:0005560 {is_inferred="true"} ! pairwise sequence alignment evidence used in manual assertion intersection_of: ECO:0000206 ! BLAST evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion [Term] id: ECO:0000031 name: protein BLAST evidence used in manual assertion def: "Protein BLAST evidence that is used in a manual assertion." [ECO:MCC] synonym: "curated protein BLAST analysis" RELATED [] xref: GO_REF:0000012 "Pairwise alignment (TIGR)" xref: GO_REF:0000027 "BLAST search criteria for ISS assignment in PAMGO_GAT" is_a: ECO:0000030 {is_inferred="true"} ! BLAST evidence used in manual assertion is_a: ECO:0000208 ! protein BLAST evidence intersection_of: ECO:0000208 ! protein BLAST evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion [Term] id: ECO:0000032 name: nucleotide BLAST evidence used in manual assertion def: "Nucleotide BLAST evidence that is used in a manual assertion." [ECO:MCC] synonym: "curated nucleic acid BLAST analysis" RELATED [] is_a: ECO:0000030 {is_inferred="true"} ! BLAST evidence used in manual assertion is_a: ECO:0000207 ! nucleotide BLAST evidence intersection_of: ECO:0000207 ! nucleotide BLAST evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion [Term] id: ECO:0000033 name: traceable author statement def: "A type of author statement that is based on a cited reference." [ECO:MCC, GO:TAS] comment: The traceable author statement (TAS) evidence code covers author statements that are attributed to a cited source. Typically this type of information comes from review articles. Material from the introductions and discussion sections of non-review papers may also be suitable if another reference is cited as the source of experimental work or analysis. When annotating with this code the curator should use caution and be aware that authors often cite papers dealing with experiments that were performed in organisms different from the one being discussed in the paper at hand. Thus a problem with the TAS code is that it may turn out from following up the references in the paper that no experiments were performed on the gene in the organism actually being characterized in the primary paper. For this reason we recommend (when time and resources allow) that curators track down the cited paper and annotate directly from the experimental paper using the appropriate experimental evidence code. When this is not possible and it is necessary to annotate from reviews, the TAS code is the appropriate code to use for statements that are associated with a cited reference. Once an annotation has been made to a given term using an experimental evidence code, we recommend removing any annotations made to the same term using the TAS evidence code. is_a: ECO:0000204 ! author statement disjoint_from: ECO:0000034 ! non-traceable author statement [Term] id: ECO:0000034 name: non-traceable author statement def: "An author statement that is not associated with results presented or a cited reference." [ECO:MCC] comment: The non-traceable author statement evidence code should be used in all cases where the author makes a statement that a curator wants to capture but for which there are neither results presented nor a specific reference cited in the source used to make the annotation. The source of the information may be peer reviewed papers, textbooks, database records or vouchered specimens. is_a: ECO:0000204 ! author statement [Term] id: ECO:0000035 name: no biological data found def: "A curator inference that results from research where no information about a biological feature was found in the scientific literature, at biological databases, or within other resources." [ECO:MCC, GO:ND] comment: An assertion of "no biological data found" carries the assumption that a more-or-less exhaustive search has been conducted. is_a: ECO:0000205 ! curator inference [Term] id: ECO:0000037 name: not_recorded def: "An evidence that reflects an annotation was made before curators began tracking evidence types." [ECO:MCC, GO:NR] comment: The evidence not_recorded appears in some legacy annotations; it should not be used for new annotations. synonym: "not recorded" EXACT [GO:NR] synonym: "NR" EXACT [GO:NR] is_obsolete: true [Term] id: ECO:0000038 name: transient rescue experiment evidence is_a: ECO:0000012 ! functional complementation evidence [Term] id: ECO:0000039 name: protein assay evidence is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000040 name: immunological assay evidence is_a: ECO:0000023 ! affinity evidence [Term] id: ECO:0000041 name: similarity evidence def: "An evidence type that is based on comparing likeness of distinct biological entities." [ECO:MCC, PhenoScape:IS] synonym: "inferred from similarity" RELATED [] synonym: "IS" EXACT [] is_a: ECO:0000000 ! evidence [Term] id: ECO:0000042 name: gain-of-function mutant phenotype evidence is_a: ECO:0000015 ! mutant phenotype evidence [Term] id: ECO:0000044 name: sequence similarity evidence def: "A type of similarity based on biomolecular sequence." [ECO:MCC, TAIR:TED] comment: A sequence similarity analysis may involve a gene or a gene product, and it could be based on similarity to a single other gene or to a group of other genes. is_a: ECO:0000041 ! similarity evidence [Term] id: ECO:0000045 name: spatial pattern of protein expression evidence is_a: ECO:0000010 ! protein expression evidence [Term] id: ECO:0000046 name: protein expression level evidence is_a: ECO:0000010 ! protein expression evidence [Term] id: ECO:0000047 name: spatial pattern of transcript expression evidence is_a: ECO:0000009 ! transcript expression evidence [Term] id: ECO:0000048 name: transcript expression level evidence is_a: ECO:0000009 ! transcript expression evidence [Term] id: ECO:0000049 name: reporter gene assay evidence def: "Used when an annotation is made on the basis of the expression pattern of a reporter gene. The reporter gene may consist of the native promoter of the gene in question that drives the expression of a readily assayable gene product such as GUS or GFP. The reporter gene may also be a marker of a particular type of physiological response." [TAIR:TED] synonym: "IEP: expression of a reporter gene" RELATED [] is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000050 name: voucher specimen analysis evidence def: "Used when an annotation is made on the basis of for a phenotype description for a species or higher level group that given by an author who explicitly references an observation of a voucher specimen(s). Voucher specimens are defined as those specimens with permanent museum catalog numbers. Thus it would be possible for a person to examine this particular specimen and observe the annotated phenotype." [PhenoScape:DOA] synonym: "IVS" EXACT [] is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000051 name: genetic similarity evidence def: "A type of similarity based on genotype without respect to expression." [ECO:MCC, PhenoScape:IGTS] comment: A genetic similarity analysis might consider genetic markers, polymorphisms, alleles, or other characteristics sometimes considered as part of the field of traditional genetics. Although an attempt has been made to treat as distinct the concepts of "genetic", "genotypic", "genomic", and "sequence", there is considerable overlap in usage throughout the field of biology. synonym: "IGTS" EXACT [] synonym: "inferred from genetic similarity" RELATED [] is_a: ECO:0000041 ! similarity evidence [Term] id: ECO:0000052 name: suppressor/enhancer interaction evidence synonym: "IGI: 'traditional' genetic interactions (e.g. suppressors, synthetic lethals)" RELATED [] xref: TAIR:TED\:0000063 is_a: ECO:0000011 ! genetic interaction evidence [Term] id: ECO:0000053 name: computational combinatorial evidence def: "A type of combinatorial analysis where data are combined and evaluated by an algorithm." [ECO:go, ECO:MCC] comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments. xref: TAIR:TED\:0000004 is_a: ECO:0000212 ! combinatorial evidence [Term] id: ECO:0000054 name: double mutant phenotype evidence def: "Used when an annotation is made based on the phenotype of double mutant organism that contains a mutation in the gene product of interest in addition to a second mutation at an unrelated locus." [TAIT:TED] synonym: "IGI: double mutant analysis" RELATED [] is_a: ECO:0000011 ! genetic interaction evidence [Term] id: ECO:0000055 name: array experiment evidence is_a: ECO:0000008 ! expression pattern evidence [Term] id: ECO:0000056 name: epistatic interaction evidence def: "Used when an annotation is based on results of epistatis analysis of an allele of the gene in question and a mutant allele at another locus." [TAIR:TED] synonym: "IGI: epistatic interactions" RELATED [] is_a: ECO:0000011 ! genetic interaction evidence [Term] id: ECO:0000057 name: phenotypic similarity evidence def: "A type of similarity based on the expression of a genotype in an environment." [ECO:MCC, PhenoScape:IPTS] comment: Phenotype is defined as the outcome of the expression of a genotype in a given environment. A comparison might involve whole organisms or sub-parts of organisms. synonym: "inferred from phenotypic similarity" RELATED [] synonym: "IPTS" EXACT [] is_a: ECO:0000041 ! similarity evidence [Term] id: ECO:0000058 name: expression microarray evidence is_a: ECO:0000055 ! array experiment evidence [Term] id: ECO:0000059 name: experimental phenotypic evidence alt_id: ECO:0000014 def: "Experimental evidence that is based on the expression of a genotype in an environment." [ECO:MCC] synonym: "inferred from phenotype" RELATED [] is_a: ECO:0000006 ! experimental evidence [Term] id: ECO:0000060 name: positional similarity evidence def: "Used when an annotation is made based on the similarity of the location and or arrangement of structures." [PhenoScape:IPS] synonym: "IPS" EXACT [] is_a: ECO:0000057 ! phenotypic similarity evidence [Term] id: ECO:0000061 name: quantitative trait analysis evidence def: "A type of experimental phenotypic evidence where an annotation is made for a gene product that has not been cloned but is associated with a quantitative trait locus." [TAIR:tn] synonym: "IGI: quantitative trait analysis" RELATED [] is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000062 name: genomic microarray evidence is_a: ECO:0000055 ! array experiment evidence [Term] id: ECO:0000063 name: compositional similarity evidence def: "Used when an annotation is made based on the similarity of the histological makeup of structures." [PhenoScape:ICS] synonym: "ICS" EXACT [] is_a: ECO:0000057 ! phenotypic similarity evidence [Term] id: ECO:0000064 name: functional complementation in heterologous system evidence def: "Used when an annotation is made based on a functional complementation assay in which a wild-type copy of the gene in question is inserted into a heterologous organism with a mutation in the homologous gene." [TAIR:TED] synonym: "IGI: functional complementation in heterologous system" RELATED [] is_a: ECO:0000012 ! functional complementation evidence [Term] id: ECO:0000065 name: CpG island microarray evidence is_a: ECO:0000055 ! array experiment evidence [Term] id: ECO:0000066 name: yeast one-hybrid evidence def: "A type of hybrid interaction evidence that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors." [ECO:MCC, PSI-MI:MI\:0432, TAIR:TED] comment: The assay involves screening a library of candidate proteins for the ability bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to a yeast reporter gene (TAIR:TED). synonym: "IPI: yeast one-hybrid assay" RELATED [] xref: PSI-MI:MI\:0432 "one hybrid" is_a: ECO:0000025 ! hybrid interaction evidence [Term] id: ECO:0000067 name: developmental similarity evidence def: "Used when an annotation is made based on the similarity of embryological and/or post-embryonic origin of structures." [PhenoScape:IDS] synonym: "IDS" EXACT [] is_a: ECO:0000057 ! phenotypic similarity evidence [Term] id: ECO:0000068 name: yeast 2-hybrid evidence def: "Used when an annotation is based on the interaction between two proteins in a yeast two-hybrid assay. The assay involves screening a trap-tagged library with a bait-tagged vector. The library is tagged with a DNA-binding domain (trap) and the bait is tagged with a transcriptional activation domain. Interaction between a library-trap protein and the bait results in activation of a yeast reporter gene whose transcription can be experimentally assayed." [TAIR:TED] synonym: "IPI: yeast two-hybrid assay" RELATED [] is_a: ECO:0000025 ! hybrid interaction evidence [Term] id: ECO:0000069 name: differential methylation hybridization evidence is_a: ECO:0000026 ! nucleic acid hybridization evidence [Term] id: ECO:0000070 name: co-immunoprecipitation evidence def: "A type of immunoprecipitation evidence that involves precipitating two or more proteins via binding to an antibody specific to a single protein, followed by protein identification." [ECO:MCC] comment: If performing GO annotation, the interacting protein is referenced in the evidence_with column. synonym: "IPI: co-immunoprecipitation" RELATED [] xref: PSI-MI:MI\:0019 "coimmunoprecipitation" is_a: ECO:0000085 ! immunoprecipitation evidence [Term] id: ECO:0000071 name: morphological similarity evidence def: "Used when an annotation is made based on the similarity of the shape, structure or overall configuration of structures." [PhenoScape:IMS] synonym: "IMS" EXACT [] is_a: ECO:0000057 ! phenotypic similarity evidence [Term] id: ECO:0000072 name: Sos-recruitment assay evidence def: "A type of physical interaction evidence that is based on a genetic screening system that detects protein-protein interactions and is not based on a transcriptional readout." [TAIR:TED] synonym: "IPI: Sos-recruitment assay" RELATED [] is_obsolete: true [Term] id: ECO:0000073 name: experimental genomic evidence def: "Experimental evidence that characterizes an attribute of the genome underlying a gene product." [ECO:MCC] synonym: "inferred from genomic analysis" EXACT [] is_a: ECO:0000006 ! experimental evidence [Term] id: ECO:0000074 name: split-ubiquitin assay evidence def: "Used when an annotation is based on the interaction of two proteins in a split-ubiquitin assay. The bait protein is fused to the C-terminal ubiquitin (Cub) domain followed by a reporter protein, and the prey protein is fused to a mutated N terminal ubiquiting (NubG) domain. If bait and prey interact, their interaction brings the NubG and Cub domains close enough together to reconstitute split-ubiquitin, resulting in the release of the reporter protein by the action of the UBPs." [TAIR:TED] synonym: "IPI: split-ubiquitin assay" RELATED [] xref: PSI-MI:MI\:0112 "ubiquitin reconstruction" is_a: ECO:0000021 ! physical interaction evidence [Term] id: ECO:0000075 name: gene expression similarity evidence def: "Used when an annotation is made based on the similarity of expression of genes in structures." [PhenoScape:IGES] synonym: "IGES" EXACT [] is_a: ECO:0000057 ! phenotypic similarity evidence [Term] id: ECO:0000076 name: far-Western blotting evidence def: "Used when an annotation is based on the interaction of protein with another in a far-Western assay. The assay involves separating target proteins on an SDS-PAGE gel, blotting to a membrane, hybridization with a protein probe and visualization using a probe-directed antibody. The interacting protein is referenced in the evidence_with column." [TAIR:TED] synonym: "IPI: far-Western analysis" RELATED [] xref: PSI-MI:MI\:0047 is_a: ECO:0000021 ! physical interaction evidence [Term] id: ECO:0000077 name: methylation-specific polymerase chain reaction evidence synonym: "methylation-specific PCR evidence" EXACT [] is_a: ECO:0000082 ! polymerase chain reaction evidence [Term] id: ECO:0000078 name: southern hybridization evidence def: "Used when an annotation is based on the hybridization of a probe derived from the gene in question with a gene of known function/process/component. Can also be used when a probe derived from a gene of known function/process/component hybridizes with the gene in question." [TAIR:TED] synonym: "ISS: Southern blotting" RELATED [] synonym: "Southern blot" EXACT [] is_a: ECO:0005519 ! DNA detection assay evidence [Term] id: ECO:0000079 name: affinity chromatography evidence def: "A type of affinity evidence that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bond compound." [ECO:MCC, TAIR:TED] comment: "Used when an annotation is made based on affinity chromatography, which is a selective separation technique by which a compound (e.g., an antibody) is immobilized on a polymeric matrix and used to bind selectively other compounds. Following removal of the unattached components, the bound compound is displaced by changing the concentration of protons, salts, or cofactors in the eluent" (from original definition by TAIR:TED). Types of highly specific interaction might include those of antigen and antibody, enzyme and substrate, or receptor and ligand. synonym: "IDA: affinity chromatography" RELATED [] xref: PSI-MI:MI\:0004 "affinity chromatography technology" is_a: ECO:0000023 ! affinity evidence [Term] id: ECO:0000080 name: phylogenetic evidence def: "A type of similarity that indicates common ancestry." [ECO:MCC, PhenoScape:IP] synonym: "IP" EXACT [PhenoScape:IP] is_a: ECO:0000041 ! similarity evidence [Term] id: ECO:0000081 name: targeting sequence prediction evidence def: "Used when a component annotation is made based on the presence of a targeting sequence in a protein's primary sequence. The presence of the targeting sequence may be detected by computational prediction and/or the author's manual examination of the sequence." [TAIR:TED] synonym: "ISS: targeting sequence prediction" RELATED [] is_a: ECO:0000028 ! motif similarity evidence [Term] id: ECO:0000082 name: polymerase chain reaction evidence synonym: "PCR evidence" EXACT [] is_a: ECO:0000073 ! experimental genomic evidence [Term] id: ECO:0000083 name: transmembrane domain prediction evidence def: "Used when a component annotation is made based on the presence of one or more transmembrane domains in a protein's primary sequence. The presence of the transmembrane domains may be detected by computational prediction and/or the author's manual examination of the sequence." [TAIR:TED] synonym: "ISS: transmembrane domain prediction" RELATED [] is_a: ECO:0000028 ! motif similarity evidence [Term] id: ECO:0000084 name: gene neighbors evidence def: "Evidence that supports the identity of a gene product on the basis of the identity of neighboring genes." [ECO:MCC, GOC:mg] comment: Genomic cluster analyses include synteny and operon structure. synonym: "ICL" RELATED [] synonym: "inferred from genome cluster" EXACT [] xref: GO_REF:0000025 "Operon structure as IGC evidence" is_a: ECO:0000177 ! genomic context evidence created_by: mgiglio creation_date: 2009-03-20T11:55:18Z [Term] id: ECO:0000085 name: immunoprecipitation evidence def: "A type of protein assay evidence that involves precipitation of a multivalent antigen by a bivalent antibody for protein isolation." [ECO:MCC, ECO:SW, TAIR:TED] comment: Transcription factors isolated in this way can be incubated with a radiolabeled probe to demonstrate binding. synonym: "IDA: immunoprecipitation" RELATED [] is_a: ECO:0000039 ! protein assay evidence [Term] id: ECO:0000086 name: intermethylated site amplification evidence is_a: ECO:0000073 ! experimental genomic evidence [Term] id: ECO:0000087 name: immunolocalization evidence def: "A type of microscopy evidence where antibodies are used to detect the location of molecules or other structures within cells or tissues." [ECO:MCC, ECO:SN] synonym: "IDA: immunolocalization" RELATED [] is_a: ECO:0001098 ! microscopy evidence is_a: ECO:0005593 ! immunodetection assay evidence [Term] id: ECO:0000088 name: biological system reconstruction evidence def: "A type of evidence that is based on a combination of experimental evidences or existing models of that system in a related species used for the reconstruction of a biological system." [ECO:MCC, GOC:mg] comment: The biological system in question might be a multi-step process or pathway or a physical complex comprising several components. The components in the experimental evidence can come from the same species or a mix of species. The experimental evidences may be only partial or weak. synonym: "inferred from system reconstruction" RELATED [] synonym: "ISR" RELATED [] is_a: ECO:0000000 ! evidence created_by: mgiglio creation_date: 2009-03-20T12:00:17Z [Term] id: ECO:0000089 name: restriction landmark genomic scanning evidence alt_id: ECO:0001125 synonym: "restriction landmark genome scanning evidence" EXACT [] synonym: "RLGS evidence" EXACT [] is_a: ECO:0005519 ! DNA detection assay evidence [Term] id: ECO:0000090 name: immunogold labelling evidence def: "A type of immunolocalization evidence where the antibodies are labeled with colloidal gold particles whose location is then detected via microscopy." [ECO:MCC] synonym: "IDA: immunogold labelling" RELATED [] is_a: ECO:0000087 ! immunolocalization evidence [Term] id: ECO:0000092 name: epitope-tagged protein immunolocalization evidence def: "Used when an annotation is made based on immunolocalization of recombinant proteins fused with epitopes that are recognized by antibodies." [TAIR:TED] synonym: "IDA: immunolocalization of epitope-tagged protein" RELATED [] is_a: ECO:0000087 ! immunolocalization evidence [Term] id: ECO:0000093 name: oligonucleotide microarray evidence is_a: ECO:0000055 ! array experiment evidence [Term] id: ECO:0000094 name: biological assay evidence def: "Direct assay evidence that is derived by using a living organism to measure the effect of a substance, factor, or condition." [ECO:MCC, TAIR:TED] synonym: "IDA: bioassay" RELATED [] is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000095 name: cell growth assay evidence def: "A type of in vivo assay evidence that measures one or more aspects of cell growth over a specified time period to indicate an induced or repressed regulatory effect." [ECO:SW, PomBase:MAH] comment: Cell growth aspects can include growth rate and extent of growth. A cell growth curve acts as a natural reporter. synonym: "growth curve analysis" RELATED [] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-10-15T00:58:50Z [Term] id: ECO:0000096 name: electrophoretic mobility shift assay evidence def: "A type of direct assay evidence based on an electrophoretic mobility shift of macromolecules, compounds, and proteins where solutions of protein and nucleic acid are combined and the resulting mixtures are electrophoresed under native conditions through polyacrylamide or agarose gel to detect protein-nucleic acid interactions." [ECO:KIM, ECO:SW, PMID:17703195, TAIR:TED] comment: EMSA is often used for assessing TF-binding with fluorophore labelling. Protein-nucleic acid complexes generally migrate at a slower rate than the corresponding non-bonded nucleic acid. synonym: "EMSA: electrophoretic mobility shift assay" EXACT [] synonym: "Gel retardation assay" RELATED [PMID:17703195] synonym: "IDA: electrophoretic mobility shift assay" RELATED [] xref: PSI-MI:MI\:0413 "electrophoretic mobility shift assay" is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000097 name: cDNA microarray evidence is_a: ECO:0000055 ! array experiment evidence [Term] id: ECO:0000098 name: in situ hybridization evidence def: "Used when an annotation is made based on a method that hybridizes a labeled nucleic acid probe to a tissue or cell specimen in which the cellular structures are still intact. Depending on the label and protocol used, the probe may detect complementary DNA or RNA by hybridizing specifically to that gene or sequence." [TAIR:TED] synonym: "IDA: in situ hybridization" RELATED [] is_a: ECO:0000026 ! nucleic acid hybridization evidence [Term] id: ECO:0000100 name: fractionation evidence is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000101 name: Affymetrix array experiment evidence is_a: ECO:0000055 ! array experiment evidence [Term] id: ECO:0000102 name: co-fractionation evidence def: "Used when an annotation is made based on fractionation of a protein with other compounds, factors, or macromolecules." [TAIR:TED] synonym: "IDA: co-fractionation" RELATED [] is_a: ECO:0000100 ! fractionation evidence [Term] id: ECO:0000104 name: microarray RNA expression level evidence def: "Used when an annotation is made based on levels of RNA expression determined by microarray experiment." [TAIR:TED] synonym: "IEP: transcript levels (e.g. microarray data)" RELATED [] is_a: ECO:0000048 ! transcript expression level evidence is_a: ECO:0000058 ! expression microarray evidence [Term] id: ECO:0000105 name: Nimblegen array evidence is_a: ECO:0000055 ! array experiment evidence [Term] id: ECO:0000106 name: northern assay evidence def: "A type of transcript expression level evidence based on electrophoresis and probing to determine levels of RNA expression using a complementary hybridization probe on the separated RNA samples." [ECO:SW, TAIR:TED] synonym: "IEP: transcript levels (e.g. Northerns)" RELATED [] synonym: "Northern blot" EXACT [] is_a: ECO:0000048 ! transcript expression level evidence [Term] id: ECO:0000108 name: reverse transcription polymerase chain reaction transcription evidence def: "Used when an annotation is made based on levels of RNA expression determined by an RT-PCR experiment (quantitative or otherwise)." [TAIR:TED] synonym: "IEP: transcript levels (e.g. RT-PCR)" RELATED [] synonym: "RT-PCR transcription evidence" EXACT [] is_a: ECO:0000048 ! transcript expression level evidence is_a: ECO:0000109 ! reverse transcription polymerase chain reaction evidence [Term] id: ECO:0000109 name: reverse transcription polymerase chain reaction evidence def: "A type of polymerase chain reaction evidence where RNA is reverse transcribed into cDNA and amplified for product measurement in real time for transcription analysis i.e. qPCR." [ECO:SW, PMID:11013345, PMID:12901609] comment: The starting product for PCR, and therefore amplification volume, is directly correlated to the transcription rate. synonym: "qRT-PCR" EXACT [PMID:12901609] synonym: "RT-PCR evidence" EXACT [] is_a: ECO:0000082 ! polymerase chain reaction evidence [Term] id: ECO:0000110 name: RNA protection assay evidence def: "A type of nuclease protection assay evidence where mRNA is hybridized with radiolabeled RNA probes after which RNAse is added to digest the unbound, nonresistant single-stranded overhang regions, later the remaining probe target hybrids are purified and resolved on denaturing polyacrylamide gel, and quantified by autoradiography." [ECO:SW, PMID:23457339, TAIR:TED] synonym: "IEP: RNA protection assay" RELATED [] synonym: "ribonuclease protection assay" EXACT [] synonym: "RNAse protection assay" RELATED [] synonym: "RPA" NARROW [] is_a: ECO:0001106 ! nuclease protection assay evidence [Term] id: ECO:0000112 name: western blot evidence def: "A type of gel electrophoresis evidence where proteins are separated using gel electrophoresis and then they are blotted on a nitrocellulose or polyvinylidene difluoride (PVDF) membrane, later an antibody with a dye or an enzyme is added to the solution which is able to bind to its specific protein and this product is detected either by chemiluminescent detection technique or fluorescence imaging technique." [ECO:SW, PMID:23050259, TAIR:TED] comment: Western blot is used for protein detection and analysis. A mixed protein sample is separated through polyacrylamide gel electrophoresis then transferred to a membrane, such as polyvinylidene fluoride (PVDF), and labeled with protein-specific antibodies. synonym: "IEP: protein levels (e.g. Western blots)" RELATED [] synonym: "protein immunoblot" RELATED [] synonym: "Western blot expression analysis" RELATED [] is_a: ECO:0000333 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence is_a: ECO:0005593 ! immunodetection assay evidence [Term] id: ECO:0000114 name: expression library screen evidence def: "A type of protein expression evidence where a protein of interest is isolated on the basis of its hybridization to an antibody raised to a homologous protein." [ECO:MCC, TAIR:TED] synonym: "IEP: expression library screening" RELATED [] is_a: ECO:0000010 ! protein expression evidence [Term] id: ECO:0000116 name: differential hybridization evidence def: "Used when an annotation is made based on results of a differential hybridization experiment that demonstrates expression of an RNA under one condition and not another." [TAIR:TED] synonym: "IEP: differential hybridization" RELATED [] is_a: ECO:0000009 ! transcript expression evidence is_a: ECO:0000026 ! nucleic acid hybridization evidence [Term] id: ECO:0000118 name: subtractive hybridization evidence def: "Used when an annotation is made based on results of a subtractive hybridization experiment that demonstrates expression of an RNA under one condition and not another." [TAIR:TED] synonym: "IEP: subtractive hybridization" RELATED [] is_a: ECO:0000009 ! transcript expression evidence is_a: ECO:0000026 ! nucleic acid hybridization evidence [Term] id: ECO:0000120 name: over expression analysis evidence def: "Used when an annotation is made based on the analysis of the phenotype of a wild-type or mutant transgenic organism that has been engineered to overexpress the gene product in question." [TAIR:TED] synonym: "IMP: analysis of overexpression/ectopic expression phenotype" RELATED [] is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000122 name: protein localization evidence is_a: ECO:0000039 ! protein assay evidence [Term] id: ECO:0000124 name: fusion protein localization evidence is_a: ECO:0000122 ! protein localization evidence [Term] id: ECO:0000126 name: green fluorescent protein fusion protein localization evidence synonym: "GFP fusion protein localization evidence" EXACT [] synonym: "IDA: localization of GFP/YFP fusion protein" RELATED [] is_a: ECO:0000124 ! fusion protein localization evidence [Term] id: ECO:0000128 name: yellow fluorescent protein fusion protein localization evidence synonym: "IDA: localization of GFP/YFP fusion protein" RELATED [] synonym: "YFP fusion protein localization evidence" EXACT [] is_a: ECO:0000124 ! fusion protein localization evidence [Term] id: ECO:0000130 name: beta-glucuronidase fusion protein localization evidence synonym: "GUS fusion protein localization evidence" EXACT [] synonym: "GUS staining evidence" NARROW [] synonym: "IDA: localization of GUS fusion protein" RELATED [] is_a: ECO:0000124 ! fusion protein localization evidence [Term] id: ECO:0000132 name: beta-galactosidase fusion protein localization evidence synonym: "LacZ fusion protein localization evidence" EXACT [] is_a: ECO:0000124 ! fusion protein localization evidence [Term] id: ECO:0000134 name: transport assay evidence synonym: "IDA: transport assay" RELATED [] is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000136 name: nucleic acid binding evidence is_a: ECO:0000023 ! affinity evidence [Term] id: ECO:0000138 name: ribohomopolymer binding assay evidence synonym: "IDA: ribohomopolymer binding assay" RELATED [] is_a: ECO:0000136 ! nucleic acid binding evidence [Term] id: ECO:0000140 name: thin layer chromatography evidence def: "A type of chromatography evidence that uses a thin layer of adsorbent (such as silica gel, alumina, or cellulose) on a flat, inert substrate." [ECO:MCC] synonym: "IDA: thin layer chromatography" RELATED [] synonym: "TLC evidence" EXACT [] is_a: ECO:0000325 ! chromatography evidence [Term] id: ECO:0000142 name: protein:ion binding evidence is_a: ECO:0000024 ! protein binding evidence [Term] id: ECO:0000144 name: Southwestern blot evidence def: "Used when an annotation is made based on a Southwestern blot analysis, which detects DNA binding of proteins using labeled DNA as probe hybridizing to electrophoretically separated proteins." [TAIR:TED] synonym: "IDA: Southwestern analysis" RELATED [] is_a: ECO:0000136 ! nucleic acid binding evidence [Term] id: ECO:0000146 name: Northwestern blot evidence def: "Used when an annotation is made based on a Northwestern blot analysis, which detects RNA binding of proteins using labeled RNA as probe hybridizing to electrophoretically separated proteins." [TAIR:TED] synonym: "IDA: Northwestern analysis" RELATED [] is_a: ECO:0000136 ! nucleic acid binding evidence [Term] id: ECO:0000148 name: in vitro binding evidence synonym: "IDA: in vitro binding assay" RELATED [] is_obsolete: true [Term] id: ECO:0000150 name: in vitro transcription reconstitution assay evidence synonym: "IDA: in vitro reconstitution assay with recombinant protein" RELATED [] is_a: ECO:0000003 ! reconstitution assay evidence [Term] id: ECO:0000152 name: in vitro recombinant protein transcription reconstitution assay evidence synonym: "IDA: in vitro reconstitution assay with recombinant protein" RELATED [] is_a: ECO:0000150 ! in vitro transcription reconstitution assay evidence [Term] id: ECO:0000154 name: heterologous protein expression evidence def: "A type of protein expression evidence where a gene from one cell is inserted into a cell that does not typically contain that gene and heterologous protein expression is assessed." [ECO:KAV] synonym: "IDA: protein expression in heterologous system" RELATED [] is_a: ECO:0000010 ! protein expression evidence [Term] id: ECO:0000156 name: protein separation evidence is_a: ECO:0000100 ! fractionation evidence [Term] id: ECO:0000158 name: protein separation followed by direct sequencing evidence synonym: "IDA: protein separation and direct sequencing" RELATED [] is_a: ECO:0000156 ! protein separation evidence [Term] id: ECO:0000160 name: protein separation followed by fragment identification evidence synonym: "IDA: protein separation and fragment identification" RELATED [] is_a: ECO:0000156 ! protein separation evidence [Term] id: ECO:0000162 name: heterologous system uptake evidence def: "Used when an annotation is made based on small molecule update assays carried out in a heterologous system that contains a recombinant protein." [TAIR:TED] synonym: "IDA: uptake assay in heterologous system" RELATED [] is_a: ECO:0000134 ! transport assay evidence [Term] id: ECO:0000164 name: electrophysiology assay evidence def: "A type of direct assay evidence where electrical properties of cells or tissues are studied." [ECO:KAV] comment: The scale of electrophysiology assays can range from small, e.g. a single ion channel, to large, e.g. an entire organ such as a heart. is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000166 name: two-electrode voltage clamp experiment evidence def: "A type of electrophysiology assay evidence which involves using two electrodes inserted into a large cell (often a xenopus oocyte) where one electrode is used to measure the internal potential (voltage) of the cell and the other electrode is used to clamp the current." [ECO:SN, PMID:23529422] synonym: "IDA: two-electrode voltage clamp technique" RELATED [] is_a: ECO:0000164 ! electrophysiology assay evidence [Term] id: ECO:0000168 name: transcription assay is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000170 name: transcriptional activation assay def: "Used when an annotation is made based on assays that show transcriptional activation of a specific target reporter gene." [TAIR:TED] synonym: "IDA: transcriptional activation assay" RELATED [] is_a: ECO:0000168 ! transcription assay [Term] id: ECO:0000172 name: biochemical trait analysis evidence def: "Used when an annotation is made based on the analysis of a biochemical aspect of a mutant phenotype; for example, the accumulation of a biosynthetic intermediate." [TAIR:TED] synonym: "IMP: analysis of biochemical trait" RELATED [] is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000174 name: mutant physiological response evidence def: "Used when an annotation is made based on the physiological response of a mutant to an external stimulus; for example, abnormal growth of the root in response to gravity, delay in flowering in response to varying light conditions." [TAIR:TED] synonym: "IMP: analysis of physiological response" RELATED [] is_a: ECO:0000015 ! mutant phenotype evidence [Term] id: ECO:0000176 name: mutant visible phenotype evidence def: "Used when an annotation is made based on the visual examination of a mutant phenotype; for example, abnormalities in organism morphology." [TAIR:TED] synonym: "IMP: analysis of visible trait" RELATED [] is_a: ECO:0000015 ! mutant phenotype evidence [Term] id: ECO:0000177 name: genomic context evidence def: "An evidence type that is based on the location of a gene, which gives rise to a gene product, within the genome." [ECO:go, ECO:MCC] comment: This type of evidence might include identity of neighboring genes, operon structure, synteny, phylogenetic analysis, or other whole-genome analysis. is_a: ECO:0000000 ! evidence [Term] id: ECO:0000178 name: in vivo assay evidence is_a: ECO:0000094 ! biological assay evidence [Term] id: ECO:0000179 name: animal model system study evidence def: "A type of experimental evidence arising from the investigation of an animal model system." [ECO:MCC] is_a: ECO:0000006 ! experimental evidence [Term] id: ECO:0000180 name: clinical study evidence def: "A type of experimental evidence arising from a controlled investigation that uses human subjects." [ECO:MCC] is_a: ECO:0000006 ! experimental evidence [Term] id: ECO:0000181 name: in vitro assay evidence is_a: ECO:0000094 ! biological assay evidence [Term] id: ECO:0000182 name: in vitro culture assay evidence is_a: ECO:0000094 ! biological assay evidence [Term] id: ECO:0000183 name: cell-free assay evidence synonym: "in vitro assay" BROAD [] is_a: ECO:0000181 ! in vitro assay evidence [Term] id: ECO:0000184 name: enzyme inhibition evidence def: "A type of specific protein inhibition evidence where the protein under study is an enzyme." [ECO:MCC] is_a: ECO:0000005 ! enzyme assay evidence is_a: ECO:0000020 ! specific protein inhibition evidence [Term] id: ECO:0000200 name: sequence alignment evidence alt_id: ECO:00000057 def: "A type of sequence similarity evidence where a sequence alignment is the basis for making an annotation, but only when the alignment has been manually reviewed; or if the information is in a published paper, the authors have manually reviewed the evidence. Such alignments may be pairwise alignments or multiple alignments." [url:http\://www.geneontology.org/GO.evidence.shtml] is_a: ECO:0000044 ! sequence similarity evidence created_by: mchibucos creation_date: 2010-03-18T12:21:31Z [Term] id: ECO:0000201 name: sequence orthology evidence alt_id: ECO:00000060 def: "Orthology is a relationship between genes in different species indicating that the genes derive from a common ancestor. Orthology is established by multiple criteria generally including amino acid and/or nucleotide sequence comparisons and one or more of the following: phylogenetic analysis, coincident expression, conserved map location, functional complementation, immunological cross-reaction, similarity in subcellular localization, subunit structure, substrate specificity, and response to specific inhibitors." [url:http\://www.geneontology.org/GO.evidence.shtml] synonym: "ortholog evidence" RELATED [] is_a: ECO:0000044 ! sequence similarity evidence created_by: mchibucos creation_date: 2010-03-18T12:30:06Z [Term] id: ECO:0000202 name: match to sequence model evidence alt_id: ECO:00000063 def: "Sequence similarity evidence that is based on a sequence-based statistical model that predicts the function of a gene product." [ECO:MCC, url:http\://www.geneontology.org/GO.evidence.shtml] comment: Used when evidence from any kind of statistical model of a sequence or group of sequences is used to make a prediction about the function of a protein or RNA. Examples of relevant evidence are: Hidden Markov Models, PROSITE motifs, and tools such as tRNASCAN. is_a: ECO:0000044 ! sequence similarity evidence created_by: mchibucos creation_date: 2010-03-18T12:32:30Z [Term] id: ECO:0000203 name: automatic assertion alt_id: ECO:00000067 def: "An assertion method that does not involve human review." [ECO:MCC] comment: An automatic assertion is based on computationally generated information that is not reviewed by a person prior to making the assertion. For example, one common type of automatic assertion involves creating an association of evidence with an entity based on commonness of attributes of that entity and another entity for which an assertion already exists. The commonness is determined algorithmically. is_a: ECO:0000217 ! assertion method disjoint_from: ECO:0000218 ! manual assertion created_by: mchibucos creation_date: 2010-03-18T12:36:04Z [Term] id: ECO:0000204 name: author statement def: "An evidence type that is based on an assertion by the author of a paper, which is read by a curator." [ECO:MCC] is_a: ECO:0000000 ! evidence union_of: ECO:0000033 ! traceable author statement union_of: ECO:0000034 ! non-traceable author statement created_by: mchibucos creation_date: 2010-06-21T11:17:21Z [Term] id: ECO:0000205 name: curator inference def: "A type of inferential evidence that is based on a conclusion drawn by a curator." [ECO:MCC] is_a: ECO:0000361 ! inferential evidence created_by: mchibucos creation_date: 2010-08-18T05:55:12Z [Term] id: ECO:0000206 name: BLAST evidence def: "A type of pairwise sequence alignment evidence obtained with basic local alignment search tool (BLAST)." [ECO:MCC] is_a: ECO:0005554 ! pairwise sequence alignment evidence created_by: mchibucos creation_date: 2010-08-06T04:48:24Z [Term] id: ECO:0000207 name: nucleotide BLAST evidence def: "BLAST evidence from aligning nucleotide sequence with nucleotide sequence." [ECO:MCC] is_a: ECO:0000206 ! BLAST evidence created_by: mchibucos creation_date: 2010-08-06T04:49:58Z [Term] id: ECO:0000208 name: protein BLAST evidence def: "BLAST evidence from aligning amino acid or translated nucleotide sequence with amino acid or translated nucleotide sequence." [ECO:MCC] is_a: ECO:0000206 ! BLAST evidence created_by: mchibucos creation_date: 2010-08-06T04:50:45Z [Term] id: ECO:0000209 name: BLAST evidence used in automatic assertion def: "A type of BLAST evidence that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000206 ! BLAST evidence is_a: ECO:0005561 {is_inferred="true"} ! pairwise sequence alignment evidence used in automatic assertion intersection_of: ECO:0000206 ! BLAST evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-08-06T04:55:21Z [Term] id: ECO:0000210 name: nucleotide BLAST evidence used in automatic assertion def: "Nucleotide BLAST evidence that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000207 ! nucleotide BLAST evidence is_a: ECO:0000209 {is_inferred="true"} ! BLAST evidence used in automatic assertion intersection_of: ECO:0000207 ! nucleotide BLAST evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-08-06T05:09:51Z [Term] id: ECO:0000211 name: protein BLAST evidence used in automatic assertion def: "Protein BLAST evidence that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000208 ! protein BLAST evidence is_a: ECO:0000209 {is_inferred="true"} ! BLAST evidence used in automatic assertion intersection_of: ECO:0000208 ! protein BLAST evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-08-06T05:11:04Z [Term] id: ECO:0000212 name: combinatorial evidence alt_id: ECO:0000036 alt_id: ECO:0000043 def: "A type of evidence that arises out of the integration of more than one line of evidence." [ECO:MCC] comment: A key aspect of this type of evidence is that two or more pieces of information are combined to generate an emergent type of evidence not possible with the constituent pieces of evidence alone. A combinatorial analysis typically involves incorporation of different types of evidence. synonym: "inferred from in-silico analysis" RELATED [] is_a: ECO:0000000 ! evidence created_by: mchibucos creation_date: 2010-08-06T05:24:57Z [Term] id: ECO:0000213 name: combinatorial evidence used in automatic assertion def: "A type of combinatorial analysis that is used in an automatic assertion." [ECO:MCC] comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments. is_a: ECO:0000212 ! combinatorial evidence is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion intersection_of: ECO:0000212 ! combinatorial evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-08-06T05:28:33Z [Term] id: ECO:0000214 name: biological aspect of descendant evidence def: "A type of phylogenetic evidence whereby an aspect of an ancestral gene is inferred through the characterization of an aspect of a descendant gene." [ECO:MCC] comment: This type of evidence can be used in support of both positive and "not" GO annotations. It should be used to annotate ancestral genes but not extant ones. is_a: ECO:0000080 ! phylogenetic evidence created_by: mchibucos creation_date: 2010-10-05T11:16:50Z [Term] id: ECO:0000215 name: rapid divergence from ancestral sequence evidence def: "A type of phylogenetic evidence characterized by long phylogenetic tree branch lengths following a duplication event." [ECO:MCC] comment: Long branch lengths indicate that descendant sequences may have acquired different or additional functions than the ancestral sequence. is_a: ECO:0000080 ! phylogenetic evidence created_by: mchibucos creation_date: 2010-10-05T03:41:34Z [Term] id: ECO:0000216 name: phylogenetic determination of loss of key residues evidence def: "A type of phylogenetic evidence characterized by the absence of key sequence residues." [ECO:MCC] comment: The loss of certain residues important for function can indicate that a sequence lacks a particular function. is_a: ECO:0000080 ! phylogenetic evidence created_by: mchibucos creation_date: 2010-10-05T03:43:17Z [Term] id: ECO:0000217 name: assertion method def: "A means by which a statement is made about an entity." [ECO:MCC] union_of: ECO:0000203 ! automatic assertion union_of: ECO:0000218 ! manual assertion created_by: mchibucos creation_date: 2010-11-12T04:17:37Z [Term] id: ECO:0000218 name: manual assertion def: "An assertion method that involves human review." [ECO:MCC] comment: A manual assertion could be based on evidence that is generated by and interpreted by a human or it could involve human review of computationally generated information. is_a: ECO:0000217 ! assertion method created_by: mchibucos creation_date: 2010-11-12T04:20:02Z [Term] id: ECO:0000219 name: nucleotide sequencing assay evidence def: "A sequencing assay result for a biopolymer that comprises nucleotides." [ECO:MCC] synonym: "DNA sequencing" RELATED [OBI:0000626] is_a: ECO:0000220 ! sequencing assay evidence created_by: mchibucos creation_date: 2010-11-15T12:22:40Z [Term] id: ECO:0000220 name: sequencing assay evidence def: "A type of experimental evidence where the order of molecules that constitute a biopolymer is determined." [ECO:MCC, OBI:0600047] is_a: ECO:0000006 ! experimental evidence created_by: mchibucos creation_date: 2010-11-15T01:44:45Z [Term] id: ECO:0000221 name: high throughput nucleotide sequencing assay evidence def: "A nucleotide sequencing assay result that is derived from a parallelized sequencing technique." [ECO:MCC] comment: Typically thousands to millions of reads are produced in parallel. is_a: ECO:0000219 ! nucleotide sequencing assay evidence created_by: mchibucos creation_date: 2010-11-15T02:19:34Z [Term] id: ECO:0000222 name: Illumina sequencing evidence def: "A high throughput nucleotide sequencing result derived by Illumina (Solexa) sequencing technology." [ECO:MCC] comment: In Illumina sequencing technology, adapters are ligated onto the ends of randomly fragmented DNA. After single-stranded fragments are bound to the inside surface of a flow cell channel, they undergo in-place bridge PCR amplification. To begin the sequencing cycle, labeled reversible terminators, primers and DNA polymerase are added, followed by laser excitation of the fluorescent label and high resolution scanning. After non-incorporated nucleotides are washed away and the dye is chemically removed, the next cycle repeats with four labeled reversible terminators, primers and DNA polymerase, again followed by imaging. synonym: "Solexa sequencing result" RELATED [OBI:0000724] is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence created_by: mchibucos creation_date: 2010-11-15T02:38:03Z [Term] id: ECO:0000223 name: 454 pyrosequencing evidence def: "A high throughput nucleotide sequencing result derived by 454 pyrosequencing technology." [ECO:MCC] comment: 454 pyrosequencing technology amplifies individual DNA fragments inside water droplets within an oil solution (emulsion PCR). Within each droplet, a single DNA template is attached to a single primer-coated bead. Each bead is subsequently captured in a picolitre well within the sequencing machine. A luciferase-based readout system is used to identify individual nucleotides added to the nascent DNA. synonym: "pyrosequencing" BROAD [OBI:0000730] is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence created_by: mchibucos creation_date: 2010-11-15T03:50:14Z [Term] id: ECO:0000224 name: SOLiD sequencing evidence def: "A high throughput nucleotide sequencing result derived by SOLiD sequencing technology." [ECO:MCC, OBI:0000706] comment: SOLiD sequencing technology is based on sequencing by ligation. After library preparation, emulsion PCR and bead enrichment are performed, including 3' modification of templates on selected beads to allow covalent bonding to a slide. 3' modified beads are attached to a glass slide and primers are added. Four fluorescently labeled di-base probes are added and multiple cycles of ligation, detection and cleavage are performed. The number of cycles determines read length. After a number of ligation cycles, the extension product is removed and another round of ligation cycles is performed with a new primer complimentary to the n-1 position of the template. Five rounds of primer reset are performed for each sequence tag. is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence created_by: mchibucos creation_date: 2010-11-15T04:17:41Z [Term] id: ECO:0000225 name: chain termination sequencing evidence def: "A nucleotide sequencing assay result derived by the chain termination sequencing method." [ECO:MCC, OBI:0000695] comment: Chain termination sequencing, also called Sanger sequencing after its developer, uses dideoxynucleotide triphosphates (ddNTPs) as DNA chain terminators. Single-stranded DNA template, DNA primer, DNA polymerase, fluorescently or radioactively labeled nucleotides, and one of four ddNTPs are added together in each of four separate sequencing reactions. The synthesized and labeled DNA fragments from each of the four reactions are denatured, separated by size by gel electrophoresis, and visualized by autoradiography or UV light. synonym: "dye terminator sequencing" EXACT [] synonym: "Sanger sequencing" EXACT [] is_a: ECO:0000219 ! nucleotide sequencing assay evidence created_by: mchibucos creation_date: 2010-11-15T04:46:31Z [Term] id: ECO:0000226 name: chromatin immunoprecipitation evidence def: "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC] comment: Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq). synonym: "ChIP evidence" EXACT [] xref: PSI-MI:MI\:0402 "chromatin immunoprecipitation assay" is_a: ECO:0000085 ! immunoprecipitation evidence created_by: mchibucos creation_date: 2010-11-16T01:29:08Z [Term] id: ECO:0000227 name: chromatin immunoprecipitation-PCR evidence def: "A type of chromatin immunoprecipitation evidence that uses polymerase chain reaction (PCR) where specific primers are used on the pulled-down DNA for DNA detection." [ECO:MCC, ECO:SW, PMID:18388953] comment: ChIP-PCR is often used to validate ChIP-chip results and less frequently,ChIP-Seq results. synonym: "ChIP-PCR evidence" EXACT [] is_a: ECO:0000226 ! chromatin immunoprecipitation evidence created_by: mchibucos creation_date: 2010-11-16T05:02:00Z [Term] id: ECO:0000228 name: chromatin immunoprecipitation-qPCR evidence def: "A chromatin immunoprecipitation (ChIP) evidence that uses quantitative polymerase chain reaction (qPCR) for DNA detection." [ECO:MCC] synonym: "ChIP-qPCR evidence" EXACT [] is_a: ECO:0000226 ! chromatin immunoprecipitation evidence created_by: mchibucos creation_date: 2010-11-16T05:26:47Z [Term] id: ECO:0000229 name: chromatin immunoprecipitation-seq evidence def: "A type of chromatin immunoprecipitation evidence that uses high-throughput sequencing where immunoprecipitated DNA fragments are funnelled into a massively parallel sequencer to produce multiple short reads for locating binding sites of DNA-associated proteins." [ECO:MCC, ECO:SW, OBI:0000716, PMID:22955991] comment: ChIP-Seq experiments often use the input as a control to eliminate biases. To obtain similar results, ChIP-chip with high-density tiling arrays can be used. synonym: "ChIP-SEQ evidence" EXACT [OBI:0000716] synonym: "ChIP-seq evidence" EXACT [PMID:22955991] is_a: ECO:0000226 ! chromatin immunoprecipitation evidence created_by: mchibucos creation_date: 2010-11-16T05:30:41Z [Term] id: ECO:0000230 name: chromatin immunoprecipitation-chip evidence def: "A type of chromatin immunoprecipitation evidence that uses a tiling microarray for the detection of protein-bound DNA regions where chromatin is immunoprecipitated for protein tagging and DNA is sheared from the cross-linked protein-DNA complex to be arrayed revealing the protein-bound genomic regions." [ECO:MCC, ECO:SW, PMID:18388953, PMID:19381927] comment: A fixating agent such as formaldehyde is used to cross link proteins and DNA. Sonication is applied for DNA shearing. For transcription factor tagging, an antibody or epitope is employed. After cross-linking is reversed, the DNA is amplified and labeled with a flourophore for microarray which produces a resolution of aproximately 500 bp. synonym: "ChIP-chip evidence" EXACT [] synonym: "ChIP-on-chip evidence" EXACT [] xref: PSI-MI:MI\:0225 "chromatin immunoprecipitation array" is_a: ECO:0000226 ! chromatin immunoprecipitation evidence created_by: mchibucos creation_date: 2010-11-16T05:43:55Z [Term] id: ECO:0000231 name: quantitative polymerase chain reaction evidence def: "A type of polymerase chain reaction evidence where the reaction product is quantified in real-time across cycles by means of fluorescent dyes for detection and quantification of specific sequences in a DNA sample." [ECO:MCC, ECO:SW] comment: Quantitative PCR enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample. The dye is activated upon binding double-stranded DNA.\nAlthough qRT-PCR is commonly found in literature synonymous to qPCR, it is preferable not to use this synonym as it is the accepted abbreviation for reverse transcription PCR. qPCR should be used instead (ISBN 978-0-470-68386-6 page 173). synonym: "Q-PCR evidence" EXACT [] synonym: "qRT-PCR evidence" RELATED [] synonym: "quantitative PCR evidence" EXACT [] synonym: "real-time PCR evidence" EXACT [] synonym: "real-time polymerase chain reaction evidence" EXACT [] is_a: ECO:0000082 ! polymerase chain reaction evidence created_by: mchibucos creation_date: 2010-11-16T05:57:20Z [Term] id: ECO:0000232 name: chromosome conformation capture-based evidence def: "A type of experimental evidence that is based on a five-step technique of cross-linking DNA in vivo, restriction digestion, intramolecular ligation, reversing DNA cross-links, and DNA processing and quantitation." [ECO:MCC] comment: Chromosome conformation capture technologies are used to study the structural properties and spatial organization of chromosomes inside cells. All types of chromosome conformation capture technology involve five primary steps: cross-linking of DNA in vivo, restriction digestion, intramolecular ligation, reversing cross-linkages, and DNA enrichment and quantitation. The last step is different for each type of chromosome conformation capture technology, and may include PCR (3C), inverse PCR (4C), or ligation-mediated amplification (5C). Methods for product quantitation can include agarose gel detection or real-time quantitative PCR in the case of 3C, or high-throughput sequencing or microarray analysis for 4C and 5C. synonym: "3C" NARROW [] synonym: "chromosome conformation capture" NARROW [] is_a: ECO:0000073 ! experimental genomic evidence created_by: mchibucos creation_date: 2010-11-17T12:27:14Z [Term] id: ECO:0000233 name: chromosome conformation capture evidence def: "A type of chromosome conformation capture-based evidence that is derived by performing a type of polymerase chain reaction (PCR) in the final product quantitation step." [ECO:MCC] comment: 3C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C differs only in the fifth step, which involves performing either standard polymerase chain reaction (PCR) or quantitative PCR (qPCR) for product quantitation. synonym: "3C" EXACT [] is_a: ECO:0000232 ! chromosome conformation capture-based evidence created_by: mchibucos creation_date: 2010-11-17T12:46:15Z [Term] id: ECO:0000234 name: circularized chromosome conformation capture evidence def: "A type of chromosome conformation capture-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a prior circularized 3C library followed by product quantitation with a microarray." [ECO:MCC] comment: 4C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 4C differs only in the fifth step, which involves inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation. synonym: "4C" EXACT [] synonym: "chromosome conformation capture on chip" EXACT [] synonym: "circularized 3C" EXACT [] is_a: ECO:0000232 ! chromosome conformation capture-based evidence created_by: mchibucos creation_date: 2010-11-19T01:20:28Z [Term] id: ECO:0000235 name: carbon-copy chromosome conformation capture evidence def: "A type of chromosome conformation capture-based evidence that is derived by performing ligation-mediated amplification (LMA) prior to product quantitation." [ECO:MCC] comment: 5C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 5C differs only in the fifth step, which involves ligation-mediated amplification (LMA) followed by product quantitation. synonym: "5C" EXACT [] synonym: "carbon-copy 3C" EXACT [] is_a: ECO:0000232 ! chromosome conformation capture-based evidence created_by: mchibucos creation_date: 2010-11-19T01:22:20Z [Term] id: ECO:0000236 name: chromosome conformation capture-PCR evidence def: "A type of chromosome conformation capture evidence that is derived by performing polymerase chain reaction (PCR) in the quantitation step." [ECO:MCC] synonym: "3C" BROAD [] synonym: "3C-PCR" EXACT [] is_a: ECO:0000233 ! chromosome conformation capture evidence created_by: mchibucos creation_date: 2010-11-19T03:00:47Z [Term] id: ECO:0000237 name: chromosome conformation capture-qPCR evidence def: "A type of chromosome conformation capture evidence that is derived by performing quantitative polymerase chain reaction (qPCR) in the quantitation step." [ECO:MCC] synonym: "3C-qPCR" EXACT [] synonym: "3C-quantitative PCR" EXACT [] synonym: "3C-real-time PCR" EXACT [] is_a: ECO:0000233 ! chromosome conformation capture evidence created_by: mchibucos creation_date: 2010-11-19T03:06:47Z [Term] id: ECO:0000238 name: Hi-C evidence def: "A type of chromosome conformation capture-based evidence that is derived by performing immunoprecipitation enrichment of ligated fragments events prior to product quantitation." [ECO:MCC] comment: Hi-C is used to study the structural properties and spatial organization of chromosomes inside cells. Hi-C is similar to other types of chromosome conformation capture technology, but involves the addition of biotinylated nucleotides during the second or fourth step and enrichment for ligated fragments using immunoprecipitation after the fourth step. is_a: ECO:0000232 ! chromosome conformation capture-based evidence created_by: mchibucos creation_date: 2010-11-19T04:24:03Z [Term] id: ECO:0000239 name: chromosome conformation capture sequencing evidence def: "A type of chromosome conformation capture-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation with high-throughput (HT) sequencing." [ECO:MCC] comment: 3C-seq is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C-seq differs only in the fifth step, which involves inverse polymerase chain reaction (PCR) on a circularized 3C library prior to product quantitation. synonym: "3C sequencing" EXACT [] synonym: "3C-seq" EXACT [] synonym: "4C sequencing" EXACT [] synonym: "4C-seq" EXACT [] synonym: "circularized 3C sequencing" EXACT [] synonym: "circularized 3C-seq" EXACT [] is_a: ECO:0000232 ! chromosome conformation capture-based evidence created_by: mchibucos creation_date: 2010-11-30T03:22:25Z [Term] id: ECO:0000240 name: anatomical perturbation evidence def: "Experimental phenotypic evidence where a structural feature of an organism is disrupted." [ECO:MCC] is_a: ECO:0000059 ! experimental phenotypic evidence created_by: mchibucos creation_date: 2010-12-06T12:24:16Z [Term] id: ECO:0000241 name: environmental perturbation evidence def: "Experimental phenotypic evidence where the extra-organismal context of an organism is modified." [ECO:MCC] comment: Many environmental factors are subject to modification, such as temperature, pressure, humidity, radiation, and so forth. synonym: "mechanical constraint evidence" RELATED [] is_a: ECO:0000059 ! experimental phenotypic evidence created_by: mchibucos creation_date: 2010-12-06T12:33:58Z [Term] id: ECO:0000242 name: tissue ablation evidence def: "A type of anatomical perturbation that is based on disruption of tissue." [ECO:MCC] comment: A tissue ablation experiment involves observing the phenotype of an entity whose tissues have been disrupted through genetic or biochemical manipulation, physical destruction, or some other means. synonym: "ablated tissue evidence" EXACT [] is_a: ECO:0000240 ! anatomical perturbation evidence created_by: mchibucos creation_date: 2010-12-06T12:33:58Z [Term] id: ECO:0000243 name: tissue grafting evidence def: "A type of anatomical perturbation that is based on adding tissue to an organism through a graft procedure." [ECO:MCC] is_a: ECO:0000240 ! anatomical perturbation evidence created_by: mchibucos creation_date: 2010-12-06T12:33:58Z [Term] id: ECO:0000244 name: combinatorial evidence used in manual assertion def: "A type of combinatorial analysis that is used in a manual assertion." [ECO:MCC] comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments. is_a: ECO:0000212 ! combinatorial evidence is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion intersection_of: ECO:0000212 ! combinatorial evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-09T02:02:12Z [Term] id: ECO:0000245 name: computational combinatorial evidence used in manual assertion def: "A type of computational combinatorial analysis that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein subset: valid_with_protein_complex synonym: "inferred from reviewed computational analysis" EXACT [GOECO:RCA] synonym: "RCA" EXACT [GOECO:RCA] xref: GOECO:RCA "inferred from reviewed computational analysis" is_a: ECO:0000053 ! computational combinatorial evidence is_a: ECO:0000244 {is_inferred="true"} ! combinatorial evidence used in manual assertion intersection_of: ECO:0000053 ! computational combinatorial evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/rca-inferred-reviewed-computational-analysis xsd:string created_by: mchibucos creation_date: 2010-12-09T02:37:54Z [Term] id: ECO:0000246 name: computational combinatorial evidence used in automatic assertion def: "A type of computational combinatorial analysis that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000053 ! computational combinatorial evidence is_a: ECO:0000213 {is_inferred="true"} ! combinatorial evidence used in automatic assertion intersection_of: ECO:0000053 ! computational combinatorial evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-12-09T02:37:54Z [Term] id: ECO:0000247 name: sequence alignment evidence used in manual assertion def: "Sequence alignment evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein synonym: "inferred from sequence alignment" EXACT [GOECO:ISA] synonym: "ISA" EXACT [GOECO:ISA] xref: GOECO:ISA "inferred from sequence alignment" is_a: ECO:0000200 ! sequence alignment evidence is_a: ECO:0000250 {is_inferred="true"} ! sequence similarity evidence used in manual assertion intersection_of: ECO:0000200 ! sequence alignment evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/isa-inferred-sequence-alignment xsd:string created_by: mchibucos creation_date: 2010-12-09T05:12:27Z [Term] id: ECO:0000248 name: sequence alignment evidence used in automatic assertion def: "Sequence alignment evidence that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000200 ! sequence alignment evidence is_a: ECO:0000249 {is_inferred="true"} ! sequence similarity evidence used in automatic assertion intersection_of: ECO:0000200 ! sequence alignment evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-12-09T05:12:27Z [Term] id: ECO:0000249 name: sequence similarity evidence used in automatic assertion def: "Sequence similarity evidence that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000044 ! sequence similarity evidence is_a: ECO:0000251 {is_inferred="true"} ! similarity evidence used in automatic assertion intersection_of: ECO:0000044 ! sequence similarity evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-12-09T05:22:30Z [Term] id: ECO:0000250 name: sequence similarity evidence used in manual assertion def: "A type of sequence similarity evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein subset: valid_with_protein_complex synonym: "inferred from sequence or structural similarity" EXACT [GOECO:ISS] synonym: "ISS" EXACT [GOECO:ISS] xref: GOECO:ISS "inferred from sequence or structural similarity" is_a: ECO:0000044 ! sequence similarity evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000044 ! sequence similarity evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/iss-inferred-sequence-or-structural-similarity xsd:string created_by: mchibucos creation_date: 2010-12-09T05:22:30Z [Term] id: ECO:0000251 name: similarity evidence used in automatic assertion def: "Similarity evidence that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000041 ! similarity evidence is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion intersection_of: ECO:0000041 ! similarity evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-12-09T05:28:12Z [Term] id: ECO:0000252 name: similarity evidence used in manual assertion def: "Similarity evidence that is used in a manual assertion." [ECO:MCC] subset: go_groupings is_a: ECO:0000041 ! similarity evidence is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion intersection_of: ECO:0000041 ! similarity evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-09T05:28:12Z [Term] id: ECO:0000253 name: genetic similarity evidence used in manual assertion def: "Genetic similarity evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000051 ! genetic similarity evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000051 ! genetic similarity evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-10T01:42:44Z [Term] id: ECO:0000254 name: genetic similarity evidence used in automatic assertion def: "Genetic similarity evidence that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000051 ! genetic similarity evidence is_a: ECO:0000251 {is_inferred="true"} ! similarity evidence used in automatic assertion intersection_of: ECO:0000051 ! genetic similarity evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-12-10T01:42:44Z [Term] id: ECO:0000255 name: match to sequence model evidence used in manual assertion def: "A match to sequence model evidence that is employed in a manual assertion." [ECO:MCC] synonym: "inferred from sequence model" EXACT [GOECO:ISM] synonym: "ISM" EXACT [GOECO:ISM] xref: GO_REF:0000011 "Hidden Markov Models (TIGR)" xref: GOECO:ISM "inferred from sequence model" is_a: ECO:0000202 ! match to sequence model evidence is_a: ECO:0000250 {is_inferred="true"} ! sequence similarity evidence used in manual assertion intersection_of: ECO:0000202 ! match to sequence model evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/ism-inferred-sequence-model xsd:string created_by: mchibucos creation_date: 2010-12-10T02:26:35Z [Term] id: ECO:0000256 name: match to sequence model evidence used in automatic assertion def: "A match to sequence model evidence that is employed in an automatic assertion." [ECO:MCC] xref: GO_REF:0000002 "Gene Ontology annotation through association of InterPro records with GO terms." xref: GO_REF:0000107 "Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl" is_a: ECO:0000202 ! match to sequence model evidence is_a: ECO:0000249 {is_inferred="true"} ! sequence similarity evidence used in automatic assertion intersection_of: ECO:0000202 ! match to sequence model evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-12-10T02:26:35Z [Term] id: ECO:0000257 name: motif similarity evidence used in manual assertion def: "Motif similarity evidence that is employed in a manual assertion." [ECO:MCC] is_a: ECO:0000028 ! motif similarity evidence is_a: ECO:0000255 {is_inferred="true"} ! match to sequence model evidence used in manual assertion intersection_of: ECO:0000028 ! motif similarity evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-10T02:44:34Z [Term] id: ECO:0000258 name: motif similarity evidence used in automatic assertion def: "Motif similarity evidence that is employed in an automatic assertion." [ECO:MCC] is_a: ECO:0000028 ! motif similarity evidence is_a: ECO:0000256 {is_inferred="true"} ! match to sequence model evidence used in automatic assertion intersection_of: ECO:0000028 ! motif similarity evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-12-10T02:44:34Z [Term] id: ECO:0000259 name: match to InterPro member signature evidence used in automatic assertion def: "A match to InterPro member signature evidence that is employed in an automatic assertion." [ECO:MCC] is_a: ECO:0000029 ! match to InterPro member signature evidence is_a: ECO:0000256 {is_inferred="true"} ! match to sequence model evidence used in automatic assertion intersection_of: ECO:0000029 ! match to InterPro member signature evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-12-10T02:54:51Z [Term] id: ECO:0000260 name: match to InterPro member signature evidence used in manual assertion def: "A match to InterPro member signature evidence that is employed in a manual assertion." [ECO:MCC] is_a: ECO:0000029 ! match to InterPro member signature evidence is_a: ECO:0000255 {is_inferred="true"} ! match to sequence model evidence used in manual assertion intersection_of: ECO:0000029 ! match to InterPro member signature evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-10T02:54:51Z [Term] id: ECO:0000261 name: targeting sequence prediction evidence used in automatic assertion def: "A targeting sequence prediction that is employed in an automatic assertion." [ECO:MCC] is_a: ECO:0000081 ! targeting sequence prediction evidence is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion intersection_of: ECO:0000081 ! targeting sequence prediction evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-12-10T03:02:49Z [Term] id: ECO:0000262 name: targeting sequence prediction evidence used in manual assertion def: "A targeting sequence prediction that is employed in a manual assertion." [ECO:MCC] is_a: ECO:0000081 ! targeting sequence prediction evidence is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion intersection_of: ECO:0000081 ! targeting sequence prediction evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-10T03:02:49Z [Term] id: ECO:0000263 name: transmembrane domain prediction evidence used in automatic assertion def: "Transmembrane domain prediction that is employed in an automatic assertion." [ECO:MCC] is_a: ECO:0000083 ! transmembrane domain prediction evidence is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion intersection_of: ECO:0000083 ! transmembrane domain prediction evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-12-10T03:28:35Z [Term] id: ECO:0000264 name: transmembrane domain prediction evidence used in manual assertion def: "Transmembrane domain prediction that is employed in a manual assertion." [ECO:MCC] is_a: ECO:0000083 ! transmembrane domain prediction evidence is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion intersection_of: ECO:0000083 ! transmembrane domain prediction evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-10T03:28:35Z [Term] id: ECO:0000265 name: sequence orthology evidence used in automatic assertion def: "Sequence orthology evidence that is employed in an automatic assertion." [ECO:MCC] synonym: "Ortholog evidence" RELATED [] xref: GO_REF:0000019 "Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara" xref: GO_REF:0000020 "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins." xref: GO_REF:0000035 "Automatic transfer of experimentally verified manual GO annotation data to plant orthologs using Ensembl Compara" xref: GO_REF:0000049 "Automatic transfer of experimentally verified manual GO annotation data to fungal orthologs using Ensembl Compara" is_a: ECO:0000201 ! sequence orthology evidence is_a: ECO:0000249 {is_inferred="true"} ! sequence similarity evidence used in automatic assertion intersection_of: ECO:0000201 ! sequence orthology evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2010-12-10T03:39:41Z [Term] id: ECO:0000266 name: sequence orthology evidence used in manual assertion def: "A type of sequence orthology evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein subset: valid_with_protein_complex synonym: "inferred from sequence orthology" EXACT [] {name="GOECO:ISO"} synonym: "ISO" EXACT [GOECO:ISO] synonym: "Ortholog evidence" RELATED [] xref: GOECO:ISO "inferred from sequence orthology" is_a: ECO:0000201 ! sequence orthology evidence is_a: ECO:0000250 {is_inferred="true"} ! sequence similarity evidence used in manual assertion intersection_of: ECO:0000201 ! sequence orthology evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/iso-inferred-sequence-orthology xsd:string created_by: mchibucos creation_date: 2010-12-10T03:39:41Z [Term] id: ECO:0000267 name: enzyme-linked immunoabsorbent assay evidence def: "A type of protein detection assay evidence where an antigen is bound to a substrate, and an antibody directly or indirectly linked to an enzyme is utilized to determine the amount of bound antigen which is visualized with a color change in the substrate." [ECO:MCC, ECO:SW, PMID:23949770] comment: ELISA is sometimes used in the study of transcriptional regulation to identify a produced protein, especially secreted protein. synonym: "ELISA evidence" EXACT [] synonym: "enzyme-linked immunosorbent assay" NARROW [] xref: PSI-MI:MI\:0411 "enzyme linked immunosorbent assay" is_a: ECO:0005517 ! protein detection assay evidence created_by: mchibucos creation_date: 2010-12-14T01:57:13Z [Term] id: ECO:0000268 name: flow cytometry evidence def: "A direct assay that passes a stream of cells or particles past a light source, followed by using a detector to monitor light scatter or fluorochrome excitation to characterize the cells or particles." [ECO:MCC] synonym: "FCM" EXACT [] is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2010-12-14T02:07:08Z [Term] id: ECO:0000269 name: experimental evidence used in manual assertion def: "A type of experimental evidence that is used in a manual assertion." [ECO:MCC] synonym: "EXP" EXACT [GOECO:EXP] synonym: "inferred from experiment" EXACT [GOECO:EXP] xref: GOECO:EXP "inferred from experiment" is_a: ECO:0000006 ! experimental evidence is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion intersection_of: ECO:0000006 ! experimental evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/exp-inferred-experiment xsd:string created_by: mchibucos creation_date: 2010-12-20T05:36:49Z [Term] id: ECO:0000270 name: expression evidence used in manual assertion def: "A type of expression pattern evidence that is used in a manual assertion." [ECO:MCC] synonym: "IEP" EXACT [GOECO:IEP] synonym: "inferred from expression pattern" EXACT [GOECO:IEP] xref: GOECO:IEP "inferred from expression pattern" is_a: ECO:0000008 ! expression pattern evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000008 ! expression pattern evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/iep-inferred-expression-pattern xsd:string created_by: mchibucos creation_date: 2010-12-20T05:46:39Z [Term] id: ECO:0000271 name: array experiment evidence used in manual assertion def: "A type of array experiment evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000055 ! array experiment evidence is_a: ECO:0000270 {is_inferred="true"} ! expression evidence used in manual assertion intersection_of: ECO:0000055 ! array experiment evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T05:51:58Z [Term] id: ECO:0000272 name: Affymetrix array experiment evidence used in manual assertion def: "A type of Affymetrix array experiment evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000101 ! Affymetrix array experiment evidence is_a: ECO:0000271 {is_inferred="true"} ! array experiment evidence used in manual assertion intersection_of: ECO:0000101 ! Affymetrix array experiment evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T05:53:36Z [Term] id: ECO:0000273 name: cDNA microarray evidence used in manual assertion def: "A type of evidence arising from a cDNA microarray experiment that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000097 ! cDNA microarray evidence is_a: ECO:0000271 {is_inferred="true"} ! array experiment evidence used in manual assertion intersection_of: ECO:0000097 ! cDNA microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T05:55:49Z [Term] id: ECO:0000274 name: CpG island microarray evidence used in manual assertion def: "A type of evidence arising from a CpG island microarray experiment that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000065 ! CpG island microarray evidence is_a: ECO:0000271 {is_inferred="true"} ! array experiment evidence used in manual assertion intersection_of: ECO:0000065 ! CpG island microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T05:58:44Z [Term] id: ECO:0000275 name: expression microarray evidence used in manual assertion def: "A type of expression microarray evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000058 ! expression microarray evidence is_a: ECO:0000271 {is_inferred="true"} ! array experiment evidence used in manual assertion intersection_of: ECO:0000058 ! expression microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:01:26Z [Term] id: ECO:0000276 name: genomic microarray evidence used in manual assertion def: "A type of evidence arising from a genomic microarray experiment that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000062 ! genomic microarray evidence is_a: ECO:0000271 {is_inferred="true"} ! array experiment evidence used in manual assertion intersection_of: ECO:0000062 ! genomic microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:03:28Z [Term] id: ECO:0000277 name: Nimblegen array evidence used in manual assertion def: "A type of evidence arising from a Nimblegen array experiment that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000105 ! Nimblegen array evidence is_a: ECO:0000271 {is_inferred="true"} ! array experiment evidence used in manual assertion intersection_of: ECO:0000105 ! Nimblegen array evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:05:20Z [Term] id: ECO:0000278 name: oligonucleotide microarray evidence used in manual assertion def: "A type of evidence arising from an oligonucleotide microarray experiment that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000093 ! oligonucleotide microarray evidence is_a: ECO:0000271 {is_inferred="true"} ! array experiment evidence used in manual assertion intersection_of: ECO:0000093 ! oligonucleotide microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:09:45Z [Term] id: ECO:0000279 name: western blot evidence used in manual assertion def: "A type of Western blot assay that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000112 ! western blot evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000112 ! western blot evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:20:01Z [Term] id: ECO:0000280 name: protein expression level evidence used in manual assertion def: "A type of protein expression level analysis that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000046 ! protein expression level evidence is_a: ECO:0000284 {is_inferred="true"} ! protein expression evidence used in manual assertion intersection_of: ECO:0000046 ! protein expression level evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:21:54Z [Term] id: ECO:0000281 name: expression library screen evidence used in manual assertion def: "A type of expression library screen evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000114 ! expression library screen evidence is_a: ECO:0000284 {is_inferred="true"} ! protein expression evidence used in manual assertion intersection_of: ECO:0000114 ! expression library screen evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:24:05Z [Term] id: ECO:0000282 name: heterologous protein expression evidence used in manual assertion def: "A type of heterologous protein expression analysis that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000154 ! heterologous protein expression evidence is_a: ECO:0000284 {is_inferred="true"} ! protein expression evidence used in manual assertion intersection_of: ECO:0000154 ! heterologous protein expression evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:27:04Z [Term] id: ECO:0000283 name: spatial pattern of protein expression evidence used in manual assertion def: "A type of protein expression spatial pattern analysis that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000045 ! spatial pattern of protein expression evidence is_a: ECO:0000284 {is_inferred="true"} ! protein expression evidence used in manual assertion intersection_of: ECO:0000045 ! spatial pattern of protein expression evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:31:44Z [Term] id: ECO:0000284 name: protein expression evidence used in manual assertion def: "A type of protein expression analysis that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000010 ! protein expression evidence is_a: ECO:0000270 {is_inferred="true"} ! expression evidence used in manual assertion is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000010 ! protein expression evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:33:46Z [Term] id: ECO:0000285 name: microarray RNA expression level evidence used in manual assertion def: "A type of microarray RNA expression level data that is used in manual assertion." [ECO:MCC] is_a: ECO:0000104 ! microarray RNA expression level evidence is_a: ECO:0000275 {is_inferred="true"} ! expression microarray evidence used in manual assertion is_a: ECO:0000286 {is_inferred="true"} ! transcript expression level evidence used in manual assertion intersection_of: ECO:0000104 ! microarray RNA expression level evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:41:33Z [Term] id: ECO:0000286 name: transcript expression level evidence used in manual assertion def: "A type of transcript expression level data that is used in manual assertion." [ECO:MCC] is_a: ECO:0000048 ! transcript expression level evidence is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion intersection_of: ECO:0000048 ! transcript expression level evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:52:03Z [Term] id: ECO:0000287 name: differential hybridization evidence used in manual assertion def: "A type of differential hybridization experiment that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000116 ! differential hybridization evidence is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion intersection_of: ECO:0000116 ! differential hybridization evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:55:14Z [Term] id: ECO:0000288 name: RNA protection assay evidence used in manual assertion def: "A type of RNA protection assay that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000110 ! RNA protection assay evidence is_a: ECO:0001240 {is_inferred="true"} ! nuclease protection assay evidence used in manual assertion intersection_of: ECO:0000110 ! RNA protection assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:57:33Z [Term] id: ECO:0000289 name: spatial pattern of transcript expression evidence used in manual assertion def: "A type of spatial pattern of transcript expression analysis that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000047 ! spatial pattern of transcript expression evidence is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion intersection_of: ECO:0000047 ! spatial pattern of transcript expression evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T06:59:18Z [Term] id: ECO:0000290 name: subtractive hybridization evidence used in manual assertion def: "A type of subtractive hybridization experiment that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000118 ! subtractive hybridization evidence is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion intersection_of: ECO:0000118 ! subtractive hybridization evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T07:00:48Z [Term] id: ECO:0000291 name: transcript expression evidence used in manual assertion def: "A type of transcript expression evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000009 ! transcript expression evidence is_a: ECO:0000270 {is_inferred="true"} ! expression evidence used in manual assertion intersection_of: ECO:0000009 ! transcript expression evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2010-12-20T07:02:08Z [Term] id: ECO:0000292 name: morpholino experiment evidence def: "A type of experimental phenotypic evidence that uses anti-sense by introducing morpholino oligonucleotides into the cytosol of a cell." [ECO:MCC] comment: Morpholino oligonucleotides modify gene expression by blocking access of other molecules to specific, approximately 25-base-long regions of the base-pairing surfaces of ribonucleic acid (RNA). To achieve this anti-sense knockdown technique, delivery of the morpholino can be achieved via microinjection, electroporation, or another method. synonym: "anti-sense" BROAD [] is_a: ECO:0000059 ! experimental phenotypic evidence created_by: mchibucos creation_date: 2011-01-10T03:36:25Z [Term] id: ECO:0000293 name: systematic evolution of ligands by exponential amplification evidence def: "A type of evidence arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand." [ECO:MCC, url:http\://en.wikipedia.org/wiki/Systematic_Evolution_of_Ligands_by_Exponential_Enrichment] comment: SELEX begins with the synthesis of a large oligonucleotide library, either single-stranded DNA or RNA, which consists of random fixed-length sequences flanked by constant 5' and 3' ends that serve as primers. The library is exposed to a target ligand, typically a protein or small organic compound. Unbound sequences are removed, typically with affinity chromatography. Bound sequences are eluted and the nucleic acid is extracted, followed by PCR amplification (RNA is first reverse transcribed). PCR product is converted to single stranded (DNA) or transcribed (RNA). Oligonucleotides are bound to the ligand again and the process is repeated with increasing elution stringency. After several rounds of the process, recovered oligonucleotides are sequenced and analyzed to reveal the binding specificity of the protein. synonym: "in vitro evolution evidence" RELATED [] synonym: "in vitro selection evidence" EXACT [] synonym: "SELEX evidence" EXACT [] xref: PSI-MI:MI\:0657 "systematic evolution of ligands by exponential enrichment" is_a: ECO:0000021 ! physical interaction evidence created_by: mchibucos creation_date: 2011-01-10T04:23:50Z [Term] id: ECO:0000294 name: bacterial one-hybrid evidence def: "A type of hybrid interaction evidence that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey)." [ECO:MCC] comment: Bacterial one-hybrid assay is a method for screening a library of candidate proteins (prey plasmid) for the ability to bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to reporter genes (bait plasmid). Transformation of a bacterial host with two different plasmids is required: One is designed to express the "bait". The other plasmid contains a "prey" which, if bound to by the chimeric fusion product, drives expression of downstream reporter genes. synonym: "B1H evidence" EXACT [] is_a: ECO:0000025 ! hybrid interaction evidence created_by: mchibucos creation_date: 2011-01-10T06:16:20Z [Term] id: ECO:0000295 name: RNA-seq evidence def: "A type of transcript expression evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC] comment: Total RNA is isolated from cells and mRNA is purified by targeting the 3' polyadenylated (poly(A)) tail with poly(T) oligos covalently attached to a substrate. Next, cDNA fragments are generated either by hydrolysis of RNA into 200-300 base oligonucleotides, preceded by reverse transcription, or by reverse transcription initiated by random primers. The cDNA second strand is synthesized, fragments are adapter ligated and high-throughput sequencing is performed by Roche 454, Illumina, ABI-SOLiD, or another HT sequencing technology. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed. synonym: "RNA sequencing" RELATED [] synonym: "whole transcriptome shotgun sequencing" EXACT [PMID:18611170] synonym: "WTSS" EXACT [] is_a: ECO:0000009 ! transcript expression evidence created_by: mchibucos creation_date: 2011-01-11T12:54:50Z [Term] id: ECO:0000296 name: green fluorescent protein transcript localization evidence def: "A transcriptional activation assay that uses a green fluorescent protein (GFP) reporter gene." [ECO:MCC] comment: This term describes transcriptional activation; to describe immunolocalization of the protein, use the term "immunolocalization of green fluorescent protein". synonym: "localization of GFP transcript" EXACT [] is_a: ECO:0000170 ! transcriptional activation assay created_by: mchibucos creation_date: 2011-01-11T02:31:19Z [Term] id: ECO:0000297 name: LacZ transcript localization evidence def: "A transcriptional activation assay that uses a LacZ reporter gene." [ECO:MCC] comment: This term describes transcriptional activation; to describe immunolocalization of the protein, use the term "immunolocalization of LacZ protein". is_a: ECO:0000170 ! transcriptional activation assay created_by: mchibucos creation_date: 2011-01-11T02:53:19Z [Term] id: ECO:0000298 name: cleavage arrested development evidence def: "A type of experimental phenotypic evidence where embryonic development proceeds although cytokinesis is blocked." [ECO:MCC] comment: In the embryos of some organisms, inhibition of cytokinesis blocks neither the cell cycle nor the unfolding of the developmental program. As such, embryonic morphology can be frozen, allowing analysis of the developmental fate of each cell present at the time of cleavage arrest. is_a: ECO:0000059 ! experimental phenotypic evidence created_by: mchibucos creation_date: 2011-01-11T03:28:49Z [Term] id: ECO:0000299 name: cytochalasin experiment evidence def: "A type of cleavage arrested development that arises after treatment with cytochalasin." [ECO:MCC] comment: Cytochalasin inhibits cytokinesis by blocking the formation of contractile microfilaments. is_a: ECO:0000298 ! cleavage arrested development evidence created_by: mchibucos creation_date: 2011-01-11T03:30:29Z [Term] id: ECO:0000300 name: green fluorescent protein immunolocalization evidence def: "A type of immunolocalization evidence data that was generated using green fluorescent protein (GFP) as a marker." [ECO:MCC] comment: This term describes immunolocalization of the protein; to describe transcriptional activation, use the term "localization of green fluorescent protein transcript". synonym: "GFP immunolocalization evidence" EXACT [] is_a: ECO:0000087 ! immunolocalization evidence created_by: mchibucos creation_date: 2011-01-11T03:48:50Z [Term] id: ECO:0000301 name: beta-galactosidase protein immunolocalization evidence def: "A type of immunolocalization evidence that was generated using LacZ protein as a marker." [ECO:MCC] comment: This term describes immunolocalization of the protein; to describe transcriptional activation, use the term "localization of LacZ transcript". synonym: "LacZ protein immunolocalization evidence" EXACT [] is_a: ECO:0000087 ! immunolocalization evidence created_by: mchibucos creation_date: 2011-01-11T04:06:40Z [Term] id: ECO:0000302 name: author statement used in manual assertion def: "A type of author statement that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000204 ! author statement is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion intersection_of: ECO:0000204 ! author statement intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2011-01-20T02:26:47Z [Term] id: ECO:0000303 name: non-traceable author statement used in manual assertion def: "A type of non-traceable author statement that is used in a manual assertion." [ECO:MCC] synonym: "NAS" EXACT [GOECO:NAS] synonym: "non-traceable author statement" EXACT [GOECO:NAS] xref: GOECO:NAS "non-traceable author statement" is_a: ECO:0000034 ! non-traceable author statement is_a: ECO:0000302 {is_inferred="true"} ! author statement used in manual assertion intersection_of: ECO:0000034 ! non-traceable author statement intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/nas-non-traceable-author-statement xsd:string created_by: mchibucos creation_date: 2011-01-20T02:29:50Z [Term] id: ECO:0000304 name: traceable author statement used in manual assertion def: "A type of traceable author statement that is used in a manual assertion." [ECO:MCC] synonym: "TAS" EXACT [GOECO:TAS] synonym: "traceable author statement" EXACT [GOECO:TAS] xref: GOECO:TAS "traceable author statement" is_a: ECO:0000033 ! traceable author statement is_a: ECO:0000302 {is_inferred="true"} ! author statement used in manual assertion intersection_of: ECO:0000033 ! traceable author statement intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/tas-traceable-author-statement xsd:string created_by: mchibucos creation_date: 2011-01-20T02:31:03Z [Term] id: ECO:0000305 name: curator inference used in manual assertion def: "A type of curator inference that is used in a manual assertion." [ECO:MCC] subset: valid_with_biological_process subset: valid_with_cellular_component subset: valid_with_molecular_function synonym: "IC" EXACT [GOECO:IC] synonym: "inferred by curator" EXACT [GOECO:IC] xref: GOECO:IC "inferred by curator" is_a: ECO:0000205 ! curator inference is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion intersection_of: ECO:0000205 ! curator inference intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/ic-inferred-curator xsd:string created_by: mchibucos creation_date: 2011-01-20T02:33:32Z [Term] id: ECO:0000306 name: inference from background scientific knowledge used in manual assertion def: "A type of inference from background scientific knowledge that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000001 ! inference from background scientific knowledge is_a: ECO:0000305 {is_inferred="true"} ! curator inference used in manual assertion intersection_of: ECO:0000001 ! inference from background scientific knowledge intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2011-01-20T02:35:11Z [Term] id: ECO:0000307 name: no biological data found used in manual assertion def: "A type of no biological data found that is used in a manual assertion." [ECO:MCC] synonym: "ND" EXACT [GOECO:ND] synonym: "no biological data available" EXACT [GOECO:ND] xref: GOECO:ND "no biological data available" is_a: ECO:0000035 ! no biological data found is_a: ECO:0000305 {is_inferred="true"} ! curator inference used in manual assertion intersection_of: ECO:0000035 ! no biological data found intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/nd-no-biological-data-available xsd:string created_by: mchibucos creation_date: 2011-01-20T02:43:18Z [Term] id: ECO:0000308 name: biological aspect of ancestor evidence def: "A type of phylogenetic evidence whereby an aspect of a descendant gene is inferred through the characterization of an aspect of an ancestral gene." [ECO:MCC] comment: First, a characteristic of the most recent common ancestor (MRCA) is inferred from experimental evidence in descendants; this is followed by inferring a characteristic of a descendant of the MRCA. This type of evidence can be used in support of both positive and "not" GO annotations. is_a: ECO:0000080 ! phylogenetic evidence created_by: mchibucos creation_date: 2011-02-04T05:41:26Z [Term] id: ECO:0000309 name: cap analysis of gene expression evidence def: "A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by a cap-trapper system." [ECO:MCC] comment: Complementary DNA (cDNA) is synthesized from microgram quantities of mRNA using first-strand cDNA primer (oligo dT12-18) and reverse transcriptase in the presence of trehalose and sorbitol, followed by selection of full-length cDNA with biotinylated cap-trapper. Linkers are attached to the 5' ends of full-length enriched cDNAs to introduce a recognition site for the restriction endonuclease MmeI, which creates a two base overhang. After amplification, sequencing tags are concatenated for high-throughput sequencing. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed. synonym: "CAGE" EXACT [PMID:16489339] is_a: ECO:0000009 ! transcript expression evidence created_by: mchibucos creation_date: 2011-02-17T01:33:09Z [Term] id: ECO:0000310 name: nano-cap analysis of gene expression evidence def: "A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by reverse transcriptase template switching." [ECO:MCC] comment: NanoCAGE captures the 5' ends of molecules by template switching. When polymerizing the cDNA of a capped mRNA, the reverse transcriptase adds extra cytosines that are complementary to the cap. Each 5' full length cDNAs is extended upon hybridization of the riboguanosine-tailed template switching oligonucleotides to these extra cytosines. In semisuppressive PCR, the short templates fold intramolecularly and prevent the binding of primers, which precludes amplification; longer molecules are less likely to fold and are thus amplified. Templates derived from reaction artifacts form stable homoduplexes, also precluding amplification. After template switching, semisuppressive PCR, and EcoP151 cleavage, 25-bp tags are ligated to bar code-containing oligonucleotide adaptors. After PCR amplification, the nanoCAGE tags are sequenced by synthesis. NanoCAGE requires as little as 10 nanograms of total RNA. synonym: "nanoCAGE" RELATED [PMID:20543846] is_a: ECO:0000009 ! transcript expression evidence created_by: mchibucos creation_date: 2011-02-17T01:33:34Z [Term] id: ECO:0000311 name: imported information def: "An evidence type that is based on work performed by a person or group prior to a use by different person or group." [ECO:MCC] is_a: ECO:0000000 ! evidence created_by: mchibucos creation_date: 2011-03-02T05:24:13Z [Term] id: ECO:0000312 name: imported information used in manual assertion def: "A type of imported information that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000311 ! imported information is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion intersection_of: ECO:0000311 ! imported information intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2011-03-02T05:29:44Z [Term] id: ECO:0000313 name: imported information used in automatic assertion def: "A type of imported information that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000311 ! imported information is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion intersection_of: ECO:0000311 ! imported information intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2011-03-02T05:33:43Z [Term] id: ECO:0000314 name: direct assay evidence used in manual assertion def: "A type of direct assay evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_chemical_entity synonym: "IDA" RELATED [GOECO:IDA] synonym: "inferred from direct assay" EXACT [GOECO:IDA] xref: GOECO:IDA "inferred from direct assay" is_a: ECO:0000002 ! direct assay evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000002 ! direct assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/ida-inferred-direct-assay xsd:string created_by: mchibucos creation_date: 2011-10-28T04:59:31Z [Term] id: ECO:0000315 name: mutant phenotype evidence used in manual assertion def: "A type of mutant phenotype evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_chemical_entity subset: valid_with_gene subset: valid_with_protein subset: valid_with_protein_complex synonym: "IMP" RELATED [GOECO:IMP] synonym: "inferred from mutant phenotype" RELATED [GOECO:IMP] xref: GOECO:IMP "inferred from mutant phenotype" is_a: ECO:0000015 ! mutant phenotype evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000015 ! mutant phenotype evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/imp-inferred-mutant-phenotype xsd:string created_by: mchibucos creation_date: 2011-10-28T05:12:49Z [Term] id: ECO:0000316 name: genetic interaction evidence used in manual assertion def: "A type of genetic interaction experiment evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein subset: valid_with_protein_complex synonym: "IGI" EXACT [GOECO:IGI] synonym: "inferred from genetic interaction" EXACT [GOECO:IGI] xref: GOECO:IGI "inferred from genetic interaction" is_a: ECO:0000011 ! genetic interaction evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000011 ! genetic interaction evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/igi-inferred-genetic-interaction xsd:string created_by: mchibucos creation_date: 2011-10-28T05:14:42Z [Term] id: ECO:0000317 name: genomic context evidence used in manual assertion def: "A type of genomic context evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein synonym: "IGC" EXACT [GOECO:IGC] synonym: "inferred from genomic context" EXACT [GOECO:IGC] xref: GOECO:IGC "inferred from genomic context" is_a: ECO:0000177 ! genomic context evidence is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion intersection_of: ECO:0000177 ! genomic context evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/igc-inferred-genomic-context xsd:string created_by: mchibucos creation_date: 2011-10-28T05:22:53Z [Term] id: ECO:0000318 name: biological aspect of ancestor evidence used in manual assertion def: "A type of biological aspect of ancestor evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein synonym: "IBA" EXACT [GOECO:IBA] synonym: "inferred from biological aspect of ancestor" EXACT [GOECO:IBA] xref: GOECO:IBA "inferred from biological aspect of ancestor" is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion is_a: ECO:0000308 ! biological aspect of ancestor evidence intersection_of: ECO:0000308 ! biological aspect of ancestor evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/iba-inferred-biological-aspect-ancestor xsd:string created_by: mchibucos creation_date: 2011-10-28T05:23:51Z [Term] id: ECO:0000319 name: biological aspect of descendant evidence used in manual assertion def: "A type of biological aspect of descendant evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein synonym: "IBD" EXACT [GOECO:IBD] synonym: "inferred from biological aspect of descendant" EXACT [GOECO:IBD] xref: GOECO:IBD "inferred from biological aspect of descendant" is_a: ECO:0000214 ! biological aspect of descendant evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000214 ! biological aspect of descendant evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/ibd-inferred-biological-aspect-descendent xsd:string created_by: mchibucos creation_date: 2011-10-28T05:25:09Z [Term] id: ECO:0000320 name: phylogenetic determination of loss of key residues evidence used in manual assertion def: "A type of phylogenetic determination of loss of key residues evidence that is used in a manual assertion." [ECO:MCC] synonym: "IKR" EXACT [GOECO:IKR] synonym: "IMR" EXACT [GOECO:IKR] synonym: "inferred from key residues" EXACT [GOECO:IKR] synonym: "inferred from missing residues" EXACT [GOECO:IKR] xref: GOECO:IKR "inferred from key residues" xref: GOECO:IMR "inferred from missing residues" is_a: ECO:0000216 ! phylogenetic determination of loss of key residues evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000216 ! phylogenetic determination of loss of key residues evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/ikr-inferred-key-residues xsd:string created_by: mchibucos creation_date: 2011-10-28T05:25:48Z [Term] id: ECO:0000321 name: rapid divergence from ancestral sequence evidence used in manual assertion def: "A type of rapid divergence from ancestral sequence evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein synonym: "inferred from rapid divergence" EXACT [GOECO:IRD] synonym: "IRD" EXACT [GOECO:IRD] xref: GOECO:IRD "inferred from rapid divergence" is_a: ECO:0000215 ! rapid divergence from ancestral sequence evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000215 ! rapid divergence from ancestral sequence evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/ird-inferred-rapid-divergence xsd:string created_by: mchibucos creation_date: 2011-10-28T05:26:42Z [Term] id: ECO:0000322 name: imported manually asserted information used in automatic assertion def: "Evidence that was initially used in manual assertion by one person or group, which is imported by a second person or group and used in automatic assertion." [ECO:MCC] xref: GO_REF:0000037 "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries." xref: GO_REF:0000039 "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries." xref: GO_REF:0000041 "IEA (UniProt UniPathway)" is_a: ECO:0000313 ! imported information used in automatic assertion created_by: mchibucos creation_date: 2011-12-13T12:04:48Z [Term] id: ECO:0000323 name: imported automatically asserted information used in automatic assertion def: "A type of imported information used in automatic assertion that was initially used in automatic assertion by one person or group, which is imported by a second person or group and used in automatic assertion." [ECO:MCC] xref: GO_REF:0000038 "Gene Ontology annotation based on automatic assignment of UniProtKB keywords in UniProtKB/TrEMBL entries." xref: GO_REF:0000040 "Gene Ontology annotation based on the automatic assignment of UniProtKB Subcellular Location terms in UniProtKB/TrEMBL entries." is_a: ECO:0000313 ! imported information used in automatic assertion created_by: mchibucos creation_date: 2011-12-13T02:06:25Z [Term] id: ECO:0000324 name: imaging assay evidence alt_id: ECO:0001122 def: "Direct assay evidence derived from analysis of an image." [ECO:MCC] synonym: "radiologic test evidence" NARROW [] is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2012-02-23T12:01:43Z [Term] id: ECO:0000325 name: chromatography evidence def: "A type of experimental evidence that is based on separation of constituent parts of a mixture (the mobile phase) as they pass differentially through a stationary phase due to differences in partition coefficient and retention on the stationary phase." [ECO:MCC] synonym: "chromatographic evidence" EXACT [] is_a: ECO:0000006 ! experimental evidence created_by: mchibucos creation_date: 2012-05-16T11:46:27Z [Term] id: ECO:0000326 name: transcript splice pattern evidence def: "A type of sequence alignment evidence that is based on comparing the position of exon junctions, relative to a reference genome, for a transcript annotation and a supporting transcript." [ECO:MCC] is_a: ECO:0000200 ! sequence alignment evidence created_by: mchibucos creation_date: 2012-06-08T11:03:59Z [Term] id: ECO:0000327 name: whole transcript splice pattern evidence def: "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features." [ECO:MCC] comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child. is_a: ECO:0000326 ! transcript splice pattern evidence created_by: mchibucos creation_date: 2012-06-08T11:11:24Z [Term] id: ECO:0000328 name: coding sequence splice pattern evidence def: "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript." [ECO:MCC] comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child. is_a: ECO:0000326 ! transcript splice pattern evidence created_by: mchibucos creation_date: 2012-06-08T11:21:57Z [Term] id: ECO:0000329 name: whole transcript splice pattern evidence used in manual assertion def: "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in manual assertion." [ECO:MCC] comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child. is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion is_a: ECO:0000327 ! whole transcript splice pattern evidence intersection_of: ECO:0000327 ! whole transcript splice pattern evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2012-06-08T11:40:03Z [Term] id: ECO:0000330 name: coding sequence splice pattern evidence used in manual assertion def: "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in manual assertion." [ECO:MCC] comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child. is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion is_a: ECO:0000328 ! coding sequence splice pattern evidence intersection_of: ECO:0000328 ! coding sequence splice pattern evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2012-06-08T11:43:47Z [Term] id: ECO:0000331 name: coding sequence splice pattern evidence used in automatic assertion def: "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in automatic assertion." [ECO:MCC] comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child. is_a: ECO:0000248 {is_inferred="true"} ! sequence alignment evidence used in automatic assertion is_a: ECO:0000328 ! coding sequence splice pattern evidence intersection_of: ECO:0000328 ! coding sequence splice pattern evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2012-06-08T11:48:09Z [Term] id: ECO:0000332 name: whole transcript splice pattern evidence used in automatic assertion def: "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in automatic assertion." [ECO:MCC] comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child. is_a: ECO:0000248 {is_inferred="true"} ! sequence alignment evidence used in automatic assertion is_a: ECO:0000327 ! whole transcript splice pattern evidence intersection_of: ECO:0000327 ! whole transcript splice pattern evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2012-06-08T11:49:17Z [Term] id: ECO:0000333 name: sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence def: "A type of gel electrophoresis evidence where proteins are separated according to their electrophoretic mobility." [ECO:MCC, PubMed:4861258, url:http\://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html, url:http\://www.ncbi.nlm.nih.gov/Class/NAWBIS/Modules/Protein/protein18.html] comment: Electrophoretic mobility is a function of both the length and charge of a protein. The protein sample is linearized with sodium dodecyl sulfate (SDS), an anionic detergent. Typically, polypeptides bound with SDS possess an even distribution of charge per unit mass, and subsequent fractionation is by size. synonym: "SDS-PAGE evidence" EXACT [] is_a: ECO:0000337 ! gel electrophoresis evidence created_by: mchibucos creation_date: 2012-07-31T02:56:43Z [Term] id: ECO:0000334 name: particle size and count assay evidence def: "A type of direct assay evidence resulting from an assay where both the size of and number of particles in a sample are determined." [ECO:MCC] comment: An example of an instrument used to analyze particle sizes and counts is the Beckman Coulter® Z Series system. is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2012-07-31T03:19:32Z [Term] id: ECO:0000335 name: substance quantification evidence def: "A type of direct assay evidence where the quantity of a substance used as part of an assay is measured." [ECO:MCC] comment: Two examples of substance quantification assay are (i) measuring how much of a radiolabelled compound is taken up by a cell and (ii) measuring how much of a substrate is turned over by a given enzyme. is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2012-07-31T03:52:46Z [Term] id: ECO:0000336 name: competitive growth assay evidence def: "A type of biological assay evidence in which a mutated strain of a microorganism, such as a yeast or bacterium, is grown competitively with wild-type cells and the relative fitness of the strains is assessed." [GOC:MAH, PMID:14718172, PMID:20537132] synonym: "fitness profiling" EXACT [GOC:MAH, PMID:20537132] is_a: ECO:0000094 ! biological assay evidence created_by: mchibucos creation_date: 2012-08-24T12:03:58Z [Term] id: ECO:0000337 name: gel electrophoresis evidence def: "A type of direct assay evidence where molecules have been sorted according to their size and charge by moving through a gel in the presence of an electric field." [ECO:MCC, wikipedia:Gel_electrophoresis] comment: Molecules typically separated include DNA, RNA, or protein. The gel is typically made of agarose, polyacrylamide, or starch. is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2012-08-24T12:11:51Z [Term] id: ECO:0000338 name: pulsed-field gel electrophoresis evidence def: "Gel electrophoresis evidence that employs alternately pulsed, perpendicularly oriented electrical fields, at least one of which is inhomogeneous, to separate large DNA molecules on an agarose gel." [PMID:6373014] synonym: "PFGE" EXACT [] is_a: ECO:0000337 ! gel electrophoresis evidence created_by: mchibucos creation_date: 2012-08-24T12:34:01Z [Term] id: ECO:0000339 name: two-dimensional agarose gel electrophoresis evidence def: "A type of gel electrophoresis evidence where DNA molecules are electrophoresed on a low percentage agarose gel followed by high voltage electrophoresis on a higher percentage agarose gel in the presence of ethidium bromide." [PMID:16118435, PMID:8594382] comment: The first dimension gel is intentionally run at low voltage in low percentage agarose to separate DNA molecules in proportion to their mass. The second dimension is run at high voltage in a gel of higher agarose concentration in the presence of ethidium bromide so that the mobility of a non-linear molecule is drastically influenced by its shape. synonym: "2-D agarose gel electrophoresis" EXACT [] is_a: ECO:0000337 ! gel electrophoresis evidence created_by: mchibucos creation_date: 2012-08-24T12:42:37Z [Term] id: ECO:0000340 name: plasmid maintenance assay evidence def: "Experimental evidence from an assay in which a mitotically stable plasmid (or minichromosome) is introduced into cells, cells are grown without selection for several generations, the proportion of cells that retain the plasmid is then determined (by comparing colony numbers on selective versus non-selective media), and the loss per generation calculated." [ECO:MCC, PMID:6323245] synonym: "minichromosome maintenance assay evidence" EXACT [PMID:6323245] is_a: ECO:0000006 ! experimental evidence created_by: mchibucos creation_date: 2012-08-24T01:27:58Z [Term] id: ECO:0000341 name: specific protein inhibition by antibody evidence def: "A type of specific protein inhibition evidence where the molecular function of a protein is inhibited by an antibody." [ECO:MCC] is_a: ECO:0000020 ! specific protein inhibition evidence created_by: mchibucos creation_date: 2012-08-24T01:48:24Z [Term] id: ECO:0000342 name: Support of intron position by RNA-seq alignment evidence def: "A type of transcript splice pattern evidence where intron locations in predicted transcripts are compared to intron locations supported by RNA-seq evidence." [ECO:MCC] comment: RNA-seq alignment intron location evidence is used by NCBI RefSeq (http://www.ncbi.nlm.nih.gov/refseq). Exon-exon boundaries (or intron locations) of a RefSeq transcript, as determined by aligning the transcript to a reference chromosome, are compared to intron locations supported by RNAseq data. is_a: ECO:0000295 ! RNA-seq evidence created_by: mchibucos creation_date: 2013-04-03T04:22:05Z [Term] id: ECO:0000343 name: Full support of intron position by RNA-seq alignment evidence def: "A type of support of intron position by RNA-seq alignment evidence where RNA-seq alignment from a single sample supports all of the intron positions (exon pairs) predicted for a transcript." [ECO:MCC] comment: Full support entails that all exon pairs represented in the transcript are supported. For example, for a transcript containing five exons, if liver RNA-seq reads support all four introns but brain RNA-seq supports only three introns, then the liver data fully support the transcript exons whereas the brain data do not. is_a: ECO:0000342 ! Support of intron position by RNA-seq alignment evidence created_by: mchibucos creation_date: 2013-04-03T04:41:48Z [Term] id: ECO:0000344 name: Partial support of intron position by RNA-seq alignment evidence def: "A type of support of intron position by RNA-seq alignment evidence where RNA-seq alignment to a genome supports only some of the intron positions (exon pairs) predicted for a transcript." [ECO:MCC] comment: Partial support can occur in two ways. First, one or more exon-exon pair (intron) is not supported by any RNA-seq sample included in the analysis. Second, no single RNA-seq sample provides support for all exon-exon pairs (introns) represented in a transcript, but all represented exons are supported by at least one sample. is_a: ECO:0000342 ! Support of intron position by RNA-seq alignment evidence created_by: mchibucos creation_date: 2013-04-03T04:50:46Z [Term] id: ECO:0000345 name: single exon transcript confirmation via alignment evidence is_a: ECO:0000200 ! sequence alignment evidence created_by: mchibucos creation_date: 2013-04-03T05:10:34Z [Term] id: ECO:0000346 name: Support of intron position by RNA-seq alignment evidence used in manual assertion def: "A type of support of intron position by RNA-seq alignment evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion is_a: ECO:0000342 ! Support of intron position by RNA-seq alignment evidence intersection_of: ECO:0000342 ! Support of intron position by RNA-seq alignment evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2013-04-04T04:37:35Z [Term] id: ECO:0000347 name: Support of intron position by RNA-seq alignment evidence used in automatic assertion def: "A type of support of intron position by RNA-seq alignment evidence that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000342 ! Support of intron position by RNA-seq alignment evidence is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion intersection_of: ECO:0000342 ! Support of intron position by RNA-seq alignment evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2013-04-04T04:38:45Z [Term] id: ECO:0000348 name: Full support of intron position by RNA-seq alignment evidence used in automatic assertion def: "A type of full support of intron position by RNA-seq alignment evidence that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000343 ! Full support of intron position by RNA-seq alignment evidence is_a: ECO:0000347 {is_inferred="true"} ! Support of intron position by RNA-seq alignment evidence used in automatic assertion intersection_of: ECO:0000343 ! Full support of intron position by RNA-seq alignment evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2013-04-04T04:40:19Z [Term] id: ECO:0000349 name: Full support of intron position by RNA-seq alignment evidence used in manual assertion def: "A type of full support of intron position by RNA-seq alignment evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000343 ! Full support of intron position by RNA-seq alignment evidence is_a: ECO:0000346 {is_inferred="true"} ! Support of intron position by RNA-seq alignment evidence used in manual assertion intersection_of: ECO:0000343 ! Full support of intron position by RNA-seq alignment evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2013-04-04T04:41:34Z [Term] id: ECO:0000350 name: Partial support of intron position by RNA-seq alignment evidence used in automatic assertion def: "A type of partial support of intron position by RNA-seq alignment evidence that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000344 ! Partial support of intron position by RNA-seq alignment evidence is_a: ECO:0000347 {is_inferred="true"} ! Support of intron position by RNA-seq alignment evidence used in automatic assertion intersection_of: ECO:0000344 ! Partial support of intron position by RNA-seq alignment evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2013-04-04T04:42:53Z [Term] id: ECO:0000351 name: Partial support of intron position by RNA-seq alignment evidence used in manual assertion def: "A type of partial support of intron position by RNA-seq alignment evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000344 ! Partial support of intron position by RNA-seq alignment evidence is_a: ECO:0000346 {is_inferred="true"} ! Support of intron position by RNA-seq alignment evidence used in manual assertion intersection_of: ECO:0000344 ! Partial support of intron position by RNA-seq alignment evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2013-04-04T04:43:56Z [Term] id: ECO:0000352 name: evidence used in manual assertion def: "A type of evidence that is used in an manual assertion." [ECO:MCC] is_a: ECO:0000000 {is_inferred="true"} ! evidence intersection_of: ECO:0000000 ! evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos [Term] id: ECO:0000353 name: physical interaction evidence used in manual assertion def: "A type of physical interaction evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_chemical_entity subset: valid_with_gene subset: valid_with_protein subset: valid_with_protein_complex synonym: "inferred from physical interaction" EXACT [GOECO:IPI] synonym: "IPI" EXACT [GOECO:IPI] xref: GOECO:IPI "inferred from physical interaction" is_a: ECO:0000021 ! physical interaction evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000021 ! physical interaction evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion property_value: seeAlso http://geneontology.org/page/ipi-inferred-physical-interaction xsd:string created_by: mchibucos creation_date: 2013-10-31T21:01:27Z [Term] id: ECO:0000354 name: gene neighbors evidence used in manual assertion def: "A type of gene neighbors evidence that is used in a manual assertion." [ECO:MCC] xref: GO_REF:0000025 "operon structure as IGC evidence" is_a: ECO:0000084 ! gene neighbors evidence is_a: ECO:0000317 ! genomic context evidence used in manual assertion intersection_of: ECO:0000084 ! gene neighbors evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2013-11-19T13:37:24Z [Term] id: ECO:0000355 name: phylogenetic distribution evidence def: "A type of phylogenetic evidence characterized by the mapping of the character states of living organisms onto phylogenies using the method of maximum parsimony." [ECO:MCC, PMID:21238344] comment: Example of use: "The presence of paired evaginated hemispheres and olfactory bulbs in both agnathan and gnathostome radiations suggests that such hemispheres were also present in the common ancestor." PMID: 7013637. Northcutt RG. (1981) Evolution of the telencephalon in nonmammals. Ann. Rev. Neurosci. is_a: ECO:0000080 ! phylogenetic evidence created_by: mchibucos creation_date: 2014-04-22T17:02:31Z [Term] id: ECO:0000356 name: differential gene expression evidence from microarray experiment is_a: ECO:0000009 ! transcript expression evidence created_by: mchibucos creation_date: 2014-09-26T16:21:45Z [Term] id: ECO:0000357 name: differential gene expression evidence from RNA-seq experiment is_a: ECO:0000009 ! transcript expression evidence created_by: mchibucos creation_date: 2014-09-26T16:22:57Z [Term] id: ECO:0000358 name: differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) is_a: ECO:0000009 ! transcript expression evidence created_by: mchibucos creation_date: 2014-09-26T16:25:47Z [Term] id: ECO:0000359 name: differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) is_a: ECO:0000009 ! transcript expression evidence created_by: mchibucos creation_date: 2014-09-26T16:26:48Z [Term] id: ECO:0000360 name: biological target-disease association via drug is_a: ECO:0000006 ! experimental evidence created_by: mchibucos creation_date: 2014-09-26T16:39:11Z [Term] id: ECO:0000361 name: inferential evidence def: "A type of evidence where an assertion is derived from another assertion via logical inference or some non-logical but rational means." [ECO:MCC] comment: Inference is the process of deriving logical conclusions from premises known or assumed to be true, or alternatively, arriving at conclusions via some non-logical means of observation of patterns of facts, which can result in identifying new meanings and contexts for understanding. Given the evidence provided by a set of premises, deductive reasoning involves drawing conclusions that necessarily follow, i.e. are certain, whereas inductive reasoning generates conclusions that are likely to be, but are not necessarily, true. is_a: ECO:0000000 ! evidence created_by: mchibucos creation_date: 2015-07-14T11:19:37Z [Term] id: ECO:0000362 name: evidence based on computational logical inference def: "A type of inferential evidence based on a computationally derived unary inference or an inference chain in which at least one step is \ncomputationally derived." [ECO:MCC, GOC:MC] is_a: ECO:0000361 ! inferential evidence created_by: mchibucos creation_date: 2015-07-14T11:24:27Z [Term] id: ECO:0000363 name: evidence based on computational logical inference used in automatic assertion def: "A type of evidence based on computational logical inference that is used in automatic assertion." [ECO:MCC] xref: GO_REF:0000108 "Automatic assignment of GO terms using logical inference, based on on inter-ontology links" is_a: ECO:0000362 ! evidence based on computational logical inference is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion intersection_of: ECO:0000362 ! evidence based on computational logical inference intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: mchibucos creation_date: 2015-07-14T11:35:08Z [Term] id: ECO:0000364 name: evidence based on logical inference from manual annotation used in automatic assertion def: "A type of evidence based on logical inference from a manually curated annotation that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000363 ! evidence based on computational logical inference used in automatic assertion created_by: mchibucos creation_date: 2015-07-14T11:44:19Z [Term] id: ECO:0000366 name: evidence based on logical inference from automatic annotation used in automatic assertion def: "A type of evidence based on logical inference from an automatically curated annotation that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000363 ! evidence based on computational logical inference used in automatic assertion created_by: mchibucos creation_date: 2015-07-14T11:45:25Z [Term] id: ECO:0000501 name: evidence used in automatic assertion def: "A type of evidence that is used in an automatic assertion." [ECO:cjm] subset: valid_with_gene subset: valid_with_protein subset: valid_with_protein_complex synonym: "IEA" EXACT [GOECO:IEA] synonym: "inferred from electronic annotation" EXACT [GOECO:IEA] xref: GO_REF:0000003 "Gene Ontology annotation based on Enzyme Commission mapping." xref: GO_REF:0000004 "Gene Ontology annotation based on Swiss-Prot keyword mapping." xref: GO_REF:0000023 "Gene Ontology annotation based on Swiss-Prot Subcellular Location vocabulary mapping." xref: GOECO:IEA "inferred from electronic annotation" is_a: ECO:0000000 {is_inferred="true"} ! evidence intersection_of: ECO:0000000 ! evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion property_value: seeAlso http://geneontology.org/page/automatically-assigned-evidence-codes xsd:string created_by: cjm [Term] id: ECO:0001001 name: 3D cell culture evidence is_a: ECO:0005014 ! in vitro cell based assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001002 name: [3H]arachidonic acid release assay evidence is_a: ECO:0005014 ! in vitro cell based assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001003 name: [3H]-thymidine incorporation assay evidence is_a: ECO:0005008 ! DNA synthesis cell proliferation assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001004 name: 51Cr release assay evidence is_a: ECO:0005012 ! cytotoxicity assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001005 name: 7-aminoactinomycin staining evidence synonym: "7-AAD staining evidence" EXACT [] synonym: "7-amino-actinomycin D staining evidence" EXACT [] is_a: ECO:0005019 ! staining evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001006 name: adhesion assay evidence is_a: ECO:0005014 ! in vitro cell based assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001007 name: adoptive cell transfer evidence def: "A type of ex vivo assay evidence derived by ex vivo selection of tumor-reactive lymphocytes, and their activation and numerical expansion before re-infusion to the autologous tumor-bearing host." [ECO:SN, url:http\://jco.ascopubs.org/content/23/10/2346.full.pdf+html, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2305722/pdf/nihms42807.pdf] synonym: "Adoptive immunotherapy" EXACT [] synonym: "passive immunization" EXACT [] is_a: ECO:0005502 ! ex vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001008 name: Alamar blue assay evidence is_a: ECO:0005004 ! cell viability assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001009 name: allograft transplantation experiment evidence def: "A type of anatomical perturbation evidence where an organ or tissue is transplanted from one individual to another of the same species with a different genotype." [ECO:SN, url:http\://www.nature.com/subjects/allograft, url:http\://www.organdonor.gov/about/terms_and_topics/] synonym: "allografting" EXACT [] synonym: "Allotransplantation" EXACT [] synonym: "tissue grafting" NARROW [] is_a: ECO:0000240 ! anatomical perturbation evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001010 name: anion-exchange chromatography evidence def: "A type of chromatography evidence where a positively charged ion exchange resin with an affinity for molecules having net negative surface charges is used to separate the molecules." [ECO:SN, PMID:20978968, url:http\://www.bio-rad.com/en-us/applications-technologies/anion-exchange-chromatography, url:http\://www.uta.edu/faculty/sawasthi/Enzymology-4351-5324/Class%20Syllabus%20Enzymology/Ion%20Exchange%20Chromatography-1.pdf] is_a: ECO:0000325 ! chromatography evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001011 name: annexin-V staining evidence is_a: ECO:0005034 ! apoptotic assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001012 name: behavioral assay evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001013 name: blocking monoclonal antibody evidence is_a: ECO:0000040 ! immunological assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001014 name: blocking peptide evidence is_a: ECO:0000040 ! immunological assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001015 name: blocking polyclonal antibody evidence is_a: ECO:0000040 ! immunological assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001016 name: blood test evidence def: "A type of direct assay evidence where a blood sample is extracted from an organism to analyze different blood components." [ECO:SN, url:https\://www.nhlbi.nih.gov/health/health-topics/topics/bdt] is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001017 name: Boyden chamber assay evidence is_a: ECO:0005021 ! chemotaxis assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001018 name: bromodeoxyuridine incorporation assay evidence synonym: "5-bromo-2'-deoxyuridine incorporation assay evidence" EXACT [] synonym: "BrdU incorporation assay evidence" EXACT [] synonym: "BrdUrd incorporation assay evidence" EXACT [] synonym: "BUdR incorporation assay evidence" EXACT [] is_a: ECO:0001107 ! nucleotide analog incorporation evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001019 name: caspase assay evidence is_a: ECO:0005034 ! apoptotic assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001020 name: cell counting evidence is_a: ECO:0005014 ! in vitro cell based assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001021 name: cell permeability assay evidence is_a: ECO:0005014 ! in vitro cell based assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001022 name: carboxyfluorescein diacetate succinimidyl ester staining evidence synonym: "CFDA-SE staining evidence" EXACT [] synonym: "CFSE-staining evidence" EXACT [] is_a: ECO:0005019 ! staining evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001023 name: chemiluminescence-linked immunoassay evidence synonym: "CLIA evidence" EXACT [] is_a: ECO:0005517 ! protein detection assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001024 name: chimeric protein evidence is_a: ECO:0000015 ! mutant phenotype evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001025 name: co-electrophoresis evidence is_a: ECO:0000337 ! gel electrophoresis evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001026 name: co-localization evidence is_a: ECO:0000324 ! imaging assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001027 name: colony counting evidence is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001028 name: co-sedimentation assay evidence is_a: ECO:0000021 ! physical interaction evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001029 name: comet assay evidence is_a: ECO:0000337 ! gel electrophoresis evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001030 name: conditional knockout evidence is_a: ECO:0001091 ! knockout evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001031 name: conditional knockin evidence is_a: ECO:0001090 ! knockin evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001032 name: constitutively active mutant evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001033 name: cross-linking evidence is_a: ECO:0000024 ! protein binding evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001034 name: crystallography evidence is_a: ECO:0005031 ! structure determination evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001035 name: cytochemistry evidence is_a: ECO:0000008 ! expression pattern evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001036 name: cytochrome C release assay evidence is_a: ECO:0005034 ! apoptotic assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001037 name: 4',6-diamidino-2-phenylindole staining evidence synonym: "DAPI staining evidence" EXACT [] is_a: ECO:0005019 ! staining evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001038 name: deletion evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001039 name: DNA laddering assay evidence is_a: ECO:0005034 ! apoptotic assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001040 name: RNA dot blot assay evidence def: "A type of RNA detection assay evidence based on the direct application of a RNA mixture in a circular motion on a matrix for hybridization with labeled DNA fragments." [ECO:SW, PMID:21424648] synonym: "RNA dot blot" RELATED [] is_a: ECO:0005518 ! RNA detection assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001041 name: RNA microarray evidence is_a: ECO:0005518 ! RNA detection assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001042 name: dominant-negative mutant evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001043 name: Edman degradation evidence used in manual assertion def: "A type of Edman degradation evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001044 ! Edman degradation evidence intersection_of: ECO:0001044 ! Edman degradation evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:59:00Z [Term] id: ECO:0001044 name: Edman degradation evidence is_a: ECO:0005517 ! protein detection assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001045 name: eTag assay evidence is_a: ECO:0000023 ! affinity evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001046 name: filter binding assay evidence is_a: ECO:0000021 ! physical interaction evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001047 name: fluorescence in situ hybridization evidence is_a: ECO:0000008 ! expression pattern evidence is_a: ECO:0000098 ! in situ hybridization evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001048 name: fluorescence resonance energy transfer evidence synonym: "FRET" EXACT [] is_a: ECO:0000021 ! physical interaction evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001049 name: gel-filtration evidence is_a: ECO:0000325 ! chromatography evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001050 name: histochemistry evidence is_a: ECO:0000008 ! expression pattern evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001051 name: histology evidence is_a: ECO:0000324 ! imaging assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001052 name: high-performance liquid chromatography evidence synonym: "HPLC evidence" EXACT [] is_a: ECO:0000325 ! chromatography evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001053 name: immunocytochemistry evidence is_a: ECO:0005517 ! protein detection assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001054 name: immunodepletion evidence is_a: ECO:0000040 ! immunological assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001055 name: immunohistochemistry evidence is_a: ECO:0005517 ! protein detection assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001056 name: induced mutation evidence is_a: ECO:0005027 ! genetic transformation evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001057 name: in vitro acetylation assay evidence def: "A type of in vitro assay evidence where acetylated residues are detected in a compound (such as a protein or a nucleic acid) in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001058 name: in vitro cleavage assay evidence def: "A type of in vitro assay evidence where the cleavage of a protein by a protease is detected in an in vitro experiment. The cleavage gives rise to protein fragments." [ECO:SN, PMID:21121091, SIB:PG] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001059 name: in vitro deubiquitination assay evidence def: "A type of in vitro assay evidence where the removal of ubiquitin groups on a protein is detected in vitro." [ECO:SN, PMID:19692941, SIB:PG] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001060 name: in vitro deacetylation assay evidence def: "A type of in vitro assay evidence where the removal of acetyl groups from a compound is detected in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001061 name: in vitro defarnesylation assay evidence def: "A type of in vitro assay evidence where the removal of farnesyl groups from a protein is detected in an in vitro experiment." [ECO:SN, PMID:16126733, SIB:PG] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001062 name: in vitro demethylation assay evidence def: "A type of in vitro assay evidence where the removal of methyl groups from a compound is detected in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Demethylation] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001063 name: in vitro desumoylation assay evidence def: "A type of in vitro assay evidence where the removal of sumo groups is detected on a protein in vitro." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001064 name: in vitro farnesylation assay evidence def: "A type of in vitro assay evidence where farnesylated residues in proteins are detected in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Farnesyltransferase] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001065 name: in vitro methylation assay evidence def: "A type of in vitro assay evidence where methylated residues are detected in a compound (such as a protein or a nucleic acid) in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Methylation] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001066 name: in vitro palmitoylation assay evidence def: "A type of in vitro assay evidence where palmitoylated residues in proteins are detected in an in vitro experiment." [ECO:SN, PMID:17077383, SIB:PG] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001067 name: in vitro phosphatase assay evidence def: "A type of in vitro assay evidence where the removal of phosphatase groups on a protein is detected in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Phosphatase] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001068 name: in vitro protein kinase assay evidence def: "A type of in vitro assay evidence where phosphorylated residues in a protein are detected in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Protein_kinase, url:http\://en.wikipedia.org/wiki/Protein_phosphorylation] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001069 name: in vitro polyADP-ribosylation assay evidence def: "A type of in vitro assay evidence where ADP-ribosylated residues in proteins are detected in an in vitro experiment." [ECO:SN, PMID:21870253, SIB:PG] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001070 name: in vitro sumoylation assay evidence def: "A type of in vitro assay evidence where sumoylated residues are detected on a protein in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001071 name: in vitro transcription assay evidence def: "A type of in vitro assay evidence where transcription is detected in vitro to validate the activation or repression ablity of a transcription factor in the absence of other proteins present in in vivo conditions." [ECO:SN, ECO:SW, PMID:21125481, SIB:PG] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001072 name: in vitro translation assay evidence def: "A type of in vitro assay evidence where de novo protein synthesis is detected in an in vitro experiment." [ECO:SN, PMID:18230759, SIB:PG] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001073 name: in vitro ubiquitination assay evidence def: "A type of in vitro assay evidence where ubiquitinated residues are detected on a protein in an in vitro experiment." [ECO:SN, PMID:19692941, SIB:PG] is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001074 name: in vivo acetylation assay evidence def: "A type of in vivo assay evidence where acetylated residues are detected in a compound (such as a protein or a nucleic acid) in cells in vivo." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001075 name: in vivo cleavage assay evidence def: "A type of in vivo assay evidence where the cleavage of a protein by a protease is detected in cells in vivo. The cleavage gives rise to protein fragments." [ECO:SN, PMID:22154596, SIB:PG] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001076 name: in vivo deacetylation assay evidence def: "A type of in vivo assay evidence where the removal of acetyl groups from a compound is detected in cells in vivo." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001077 name: in vivo defarnesylation assay evidence def: "A type of in vivo assay evidence where removal of farnesyl groups from a protein is detected in cells in vivo." [ECO:SN, PMID:15556768, PMID:16507103, SIB:PG] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001078 name: in vivo demethylation assay evidence def: "A type of in vivo assay evidence where the removal of methyl groups from a compound is detected in cells in vivo." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Demethylation] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001079 name: in vivo deubiquitination assay evidence def: "A type of in vivo assay evidence where the removal of ubiquitin groups on a protein is detected in cells in vivo." [ECO:SN, PMID:19692941, SIB:PG] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001080 name: in vivo desumoylation assay evidence def: "A type of in vivo assay evidence where the removal of sumo groups on a protein is detected in cells in vivo." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001081 name: in vivo farnesylation assay evidence def: "A type of in vivo assay evidence where farnesylated residues in proteins are detected in cells in vivo." [ECO:SN, PMID:9030603, SIB:PG] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001082 name: in vivo methylation assay evidence def: "A type of in vivo assay evidence where methylated residues are detected in a compound (such as a protein or a nucleic acid) in vivo." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Methylation] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001083 name: in vivo palmitoylation assay evidence def: "A type of in vivo assay evidence where palmitoylated residues in proteins are detected in cells in vivo." [ECO:SN, PMID:10329400, SIB:PG] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001084 name: in vivo phosphatase assay evidence def: "A type of in vivo assay evidence where the removal of phosphate groups on a protein is detected in cells in vivo." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Phosphatase] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001085 name: in vivo protein kinase assay evidence def: "A type of in vivo assay evidence where phosphorylated residues in a protein are detected in cells in vivo." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Protein_kinase, url:http\://en.wikipedia.org/wiki/Protein_phosphorylation] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001086 name: in vivo sumoylation assay evidence def: "A type of in vivo assay evidence where sumoylated residues are detected on a protein in cells in vivo." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001087 name: in vivo transcription assay evidence def: "A type of in vivo assay evidence where transcription is detected in cells in vivo." [ECO:SN, PMID:12181418, SIB:PG] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001088 name: in vivo translation assay evidence def: "A type of in vivo assay evidence where de novo protein synthesis is detected in cells in vivo." [ECO:SN, PMID:24901308, SIB:PG] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001089 name: in vivo ubiquitination assay evidence def: "A type of in vivo assay evidence where ubiquitinated residues on a protein are detected in cells in vivo." [ECO:SN, PMID:19692941, SIB:PG] is_a: ECO:0000178 ! in vivo assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001090 name: knockin evidence is_a: ECO:0005027 ! genetic transformation evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001091 name: knockout evidence is_a: ECO:0005027 ! genetic transformation evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001092 name: lipid binding assay evidence is_a: ECO:0000021 ! physical interaction evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001093 name: luminescence-based mammalian interactome mapping assay evidence synonym: "LUMIER assay" EXACT [] is_a: ECO:0000021 ! physical interaction evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001094 name: macroscopy evidence is_a: ECO:0000006 ! experimental evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001095 name: mammalian 2-hybrid assay evidence is_a: ECO:0000025 ! hybrid interaction evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001096 name: mass spectrometry evidence is_a: ECO:0005504 ! spectrometry evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001097 name: medical imaging evidence is_a: ECO:0000324 ! imaging assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001098 name: microscopy evidence def: "A type of direct assay evidence where a microscope was used to view a sample." [ECO:MCC] is_a: ECO:0000324 ! imaging assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001099 name: motility wound healing assay evidence is_a: ECO:0000181 ! in vitro assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001100 name: MTS assay evidence synonym: "3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium" NARROW [] is_a: ECO:0005034 ! apoptotic assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001101 name: MTT assay evidence synonym: "3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide" NARROW [] is_a: ECO:0005034 ! apoptotic assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001102 name: multiplex bead-based immunoassay evidence is_a: ECO:0005517 ! protein detection assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001103 name: natural variation mutant evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001104 name: nuclear fragmentation evidence is_a: ECO:0005034 ! apoptotic assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001105 name: nuclear magnetic resonance spectroscopy evidence synonym: "NMR spectroscopy" EXACT [] is_a: ECO:0005031 ! structure determination evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001106 name: nuclease protection assay evidence is_a: ECO:0005518 ! RNA detection assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001107 name: nucleotide analog incorporation evidence is_a: ECO:0005008 ! DNA synthesis cell proliferation assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001108 name: phage display evidence is_a: ECO:0000024 ! protein binding evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001109 name: phosphoamino acid analysis evidence is_a: ECO:0000039 ! protein assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001110 name: peptide affinity enrichment evidence synonym: "phosphopeptide affinity enrichment evidence" NARROW [] is_a: ECO:0000023 ! affinity evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001111 name: physical examination evidence is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001112 name: peptide array evidence synonym: "phosphopeptide array evidence" NARROW [] is_a: ECO:0000039 ! protein assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001113 name: point mutation evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001114 name: propidium iodide staining evidence is_a: ECO:0005019 ! staining evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001115 name: fluorescence evidence is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001116 name: protein dot blot assay evidence is_a: ECO:0005517 ! protein detection assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001117 name: protein microarray evidence is_a: ECO:0005517 ! protein detection assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001118 name: protein sequencing assay evidence is_a: ECO:0000220 ! sequencing assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001119 name: quantitative mass spectrometry evidence is_a: ECO:0001096 ! mass spectrometry evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001120 name: radioisotope assay evidence synonym: "radioassay" EXACT [] synonym: "radioisotopic assay" EXACT [] is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001121 name: radioimmunoassay evidence is_a: ECO:0005517 ! protein detection assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001123 name: resonant mirror biosensor evidence is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001124 name: restriction fragment detection evidence is_a: ECO:0005519 ! DNA detection assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001126 name: spectrophotometry evidence is_a: ECO:0005504 ! spectrometry evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001127 name: surface plasmon resonance evidence def: "A type of physical interaction evidence based on real-time, rapid plasmon generation on the interface between a planar surface and vacuum that measures changes in refractive index close to the sensor surface when an analyte and its immobilized ligand bind to observe and characterize molecular interaction." [ECO:SW, PMID:11578932, PMID:19151937, PMID:8574707] comment: Plasmons are generated by an incident light beam. synonym: "SPR evidence" EXACT [PMID:11578932] is_a: ECO:0000021 ! physical interaction evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001128 name: syngeneic transplantation experiment evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001129 name: TACE activity assay evidence synonym: "Tumor necrosis factor - alpha converting enzyme activity assay" EXACT [] is_a: ECO:0000005 ! enzyme assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001130 name: tissue microarray evidence is_a: ECO:0000055 ! array experiment evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001131 name: transgenic organism evidence is_a: ECO:0005027 ! genetic transformation evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001132 name: tryptic phosphopeptide mapping assay evidence is_a: ECO:0001096 ! mass spectrometry evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001133 name: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence synonym: "TdT-mediated dUTP-biotin nick end labeling assay" EXACT [] synonym: "terminal deoxynucleotidyl transferase-dUTP nick end labeling assay evidence" EXACT [] synonym: "TUNEL assay evidence" EXACT [] is_a: ECO:0005034 ! apoptotic assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001134 name: urine test evidence is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001135 name: wound healing assay evidence is_a: ECO:0005014 ! in vitro cell based assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001136 name: WST-1 assay evidence is_a: ECO:0005007 ! cell proliferation assay evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001137 name: xenotransplantation experiment evidence def: "A type of anatomical perturbation evidence where live cells, tissues, or organs from a non-human animal source are transplanted, implanted or infused into a human recipient." [ECO:SN, url:http\://ilarjournal.oxfordjournals.org/content/37/1/16.full.pdf+html, url:http\://www.fda.gov/BiologicsBloodVaccines/Xenotransplantation/] synonym: "tissue grafting" NARROW [] synonym: "Xenograft transplantation" EXACT [] synonym: "Xenografting" EXACT [] synonym: "Xenotransplantation" EXACT [] is_a: ECO:0000240 ! anatomical perturbation evidence created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001138 name: 3D cell culture evidence used in manual assertion def: "A type of 3D cell culture evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001001 ! 3D cell culture evidence intersection_of: ECO:0001001 ! 3D cell culture evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T15:18:35Z [Term] id: ECO:0001139 name: 51Cr release assay evidence used in manual assertion def: "A type of 51Cr release assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001004 ! 51Cr release assay evidence intersection_of: ECO:0001004 ! 51Cr release assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T15:20:55Z [Term] id: ECO:0001140 name: 7-aminoactinomycin staining evidence used in manual assertion def: "A type of 7-aminoactinomycin staining evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001005 ! 7-aminoactinomycin staining evidence intersection_of: ECO:0001005 ! 7-aminoactinomycin staining evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T15:22:01Z [Term] id: ECO:0001141 name: [3H]-thymidine incorporation assay evidence used in manual assertion def: "A type of [3H]-thymidine incorporation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001003 ! [3H]-thymidine incorporation assay evidence intersection_of: ECO:0001003 ! [3H]-thymidine incorporation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T15:31:52Z [Term] id: ECO:0001142 name: [3H]arachidonic acid release assay evidence used in manual assertion def: "A type of [3H]arachidonic acid release assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001002 ! [3H]arachidonic acid release assay evidence intersection_of: ECO:0001002 ! [3H]arachidonic acid release assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T15:32:44Z [Term] id: ECO:0001143 name: adhesion assay evidence used in manual assertion def: "A type of adhesion assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001006 ! adhesion assay evidence intersection_of: ECO:0001006 ! adhesion assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T15:33:38Z [Term] id: ECO:0001144 name: adoptive cell transfer evidence used in manual assertion def: "A type of adoptive cell transfer evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001007 ! adoptive cell transfer evidence intersection_of: ECO:0001007 ! adoptive cell transfer evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T15:36:19Z [Term] id: ECO:0001145 name: Alamar blue assay evidence used in manual assertion def: "A type of Alamar blue assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001008 ! Alamar blue assay evidence intersection_of: ECO:0001008 ! Alamar blue assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T15:37:21Z [Term] id: ECO:0001146 name: allograft transplantation experiment evidence used in manual assertion def: "A type of allograft transplantation experiment evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001009 ! allograft transplantation experiment evidence intersection_of: ECO:0001009 ! allograft transplantation experiment evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T15:38:22Z [Term] id: ECO:0001147 name: anion-exchange chromatography evidence used in manual assertion def: "A type of anion-exchange chromatography evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001010 ! anion-exchange chromatography evidence intersection_of: ECO:0001010 ! anion-exchange chromatography evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T15:44:30Z [Term] id: ECO:0001148 name: annexin-V staining evidence used in manual assertion def: "A type of annexin-V staining evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001011 ! annexin-V staining evidence intersection_of: ECO:0001011 ! annexin-V staining evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T15:52:01Z [Term] id: ECO:0001149 name: behavioral assay evidence used in manual assertion def: "A type of behavioral assay evidence used that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001012 ! behavioral assay evidence intersection_of: ECO:0001012 ! behavioral assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T15:53:38Z [Term] id: ECO:0001150 name: blocking monoclonal antibody evidence used in manual assertion def: "A type of blocking monoclonal antibody evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001013 ! blocking monoclonal antibody evidence intersection_of: ECO:0001013 ! blocking monoclonal antibody evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T15:54:44Z [Term] id: ECO:0001151 name: blocking peptide evidence used in manual assertion def: "A type of blocking peptide evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001014 ! blocking peptide evidence intersection_of: ECO:0001014 ! blocking peptide evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T15:55:33Z [Term] id: ECO:0001152 name: blocking polyclonal antibody evidence used in manual assertion def: "A type of blocking polyclonal antibody evidence tthat is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001015 ! blocking polyclonal antibody evidence intersection_of: ECO:0001015 ! blocking polyclonal antibody evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:01:39Z [Term] id: ECO:0001153 name: blood test evidence used in manual assertion def: "A type of blood test evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001016 ! blood test evidence intersection_of: ECO:0001016 ! blood test evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:02:47Z [Term] id: ECO:0001154 name: Boyden chamber assay evidence used in manual assertion def: "A type of Boyden chamber assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001017 ! Boyden chamber assay evidence intersection_of: ECO:0001017 ! Boyden chamber assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:03:53Z [Term] id: ECO:0001155 name: bromodeoxyuridine incorporation assay evidence used in manual assertion def: "A type of BrdU incorporation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001018 ! bromodeoxyuridine incorporation assay evidence is_a: ECO:0001241 {is_inferred="true"} ! nucleotide analog incorporation evidence used in manual assertion intersection_of: ECO:0001018 ! bromodeoxyuridine incorporation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:04:49Z [Term] id: ECO:0001156 name: caspase assay evidence used in manual assertion def: "A type of caspase assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001019 ! caspase assay evidence intersection_of: ECO:0001019 ! caspase assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:06:06Z [Term] id: ECO:0001157 name: cell counting evidence used in manual assertion def: "A type of cell counting evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001020 ! cell counting evidence intersection_of: ECO:0001020 ! cell counting evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:07:13Z [Term] id: ECO:0001158 name: cell permeability assay evidence used in manual assertion def: "A type of cell permeability assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001021 ! cell permeability assay evidence intersection_of: ECO:0001021 ! cell permeability assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:07:58Z [Term] id: ECO:0001159 name: carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion def: "A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001022 ! carboxyfluorescein diacetate succinimidyl ester staining evidence intersection_of: ECO:0001022 ! carboxyfluorescein diacetate succinimidyl ester staining evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:08:54Z [Term] id: ECO:0001160 name: chemiluminescence-linked immunoassay evidence used in manual assertion def: "A type of chemiluminescence-linked immunoassay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001023 ! chemiluminescence-linked immunoassay evidence intersection_of: ECO:0001023 ! chemiluminescence-linked immunoassay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:09:55Z [Term] id: ECO:0001161 name: chimeric protein evidence used in manual assertion def: "A type of chimeric protein evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0001024 ! chimeric protein evidence intersection_of: ECO:0001024 ! chimeric protein evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:10:40Z [Term] id: ECO:0001162 name: co-electrophoresis evidence used in manual assertion def: "A type of co-electrophoresis evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001025 ! co-electrophoresis evidence intersection_of: ECO:0001025 ! co-electrophoresis evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:11:22Z [Term] id: ECO:0001163 name: co-localization evidence used in manual assertion def: "A type of co-localization evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001026 ! co-localization evidence is_a: ECO:0001256 {is_inferred="true"} ! imaging assay evidence used in manual assertion intersection_of: ECO:0001026 ! co-localization evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:12:04Z [Term] id: ECO:0001164 name: co-sedimentation assay evidence used in manual assertion def: "A type of co-sedimentation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001028 ! co-sedimentation assay evidence intersection_of: ECO:0001028 ! co-sedimentation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:12:55Z [Term] id: ECO:0001165 name: colony counting evidence used in manual assertion def: "A type of colony counting evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001027 ! colony counting evidence intersection_of: ECO:0001027 ! colony counting evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:14:57Z [Term] id: ECO:0001166 name: comet assay evidence used in manual assertion def: "A type of comet assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001029 ! comet assay evidence intersection_of: ECO:0001029 ! comet assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:16:01Z [Term] id: ECO:0001167 name: conditional knockin evidence used in manual assertion def: "A type of conditional knockin evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001031 ! conditional knockin evidence is_a: ECO:0001224 {is_inferred="true"} ! knockin evidence used in manual assertion intersection_of: ECO:0001031 ! conditional knockin evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:17:22Z [Term] id: ECO:0001168 name: conditional knockout evidence used in manual assertion def: "A type of conditional knockout evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001030 ! conditional knockout evidence is_a: ECO:0001225 {is_inferred="true"} ! knockout evidence used in manual assertion intersection_of: ECO:0001030 ! conditional knockout evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:17:58Z [Term] id: ECO:0001169 name: constitutively active mutant evidence used in manual assertion def: "A type of constitutively active mutant evidence that is used a in manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001032 ! constitutively active mutant evidence intersection_of: ECO:0001032 ! constitutively active mutant evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:20:23Z [Term] id: ECO:0001170 name: cross-linking evidence used in manual assertion def: "A type of cross-linking evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001033 ! cross-linking evidence intersection_of: ECO:0001033 ! cross-linking evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:21:29Z [Term] id: ECO:0001171 name: crystallography evidence used in manual assertion def: "A type of crystallography evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001034 ! crystallography evidence intersection_of: ECO:0001034 ! crystallography evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:22:20Z [Term] id: ECO:0001172 name: cytochemistry evidence used in manual assertion def: "A type of cytochemistry evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000270 {is_inferred="true"} ! expression evidence used in manual assertion is_a: ECO:0001035 ! cytochemistry evidence intersection_of: ECO:0001035 ! cytochemistry evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:23:10Z [Term] id: ECO:0001173 name: cytochrome C release assay evidence used in manual assertion def: "A type of cytochrome C release assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001036 ! cytochrome C release assay evidence intersection_of: ECO:0001036 ! cytochrome C release assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:23:53Z [Term] id: ECO:0001174 name: 4',6-diamidino-2-phenylindole staining evidence used in manual assertion def: "A type of 4',6-diamidino-2-phenylindole staining evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001037 ! 4',6-diamidino-2-phenylindole staining evidence intersection_of: ECO:0001037 ! 4',6-diamidino-2-phenylindole staining evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:25:03Z [Term] id: ECO:0001175 name: deletion evidence used in manual assertion def: "A type of deletion evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001038 ! deletion evidence intersection_of: ECO:0001038 ! deletion evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:25:54Z [Term] id: ECO:0001176 name: DNA laddering assay evidence used in manual assertion def: "A type of DNA laddering assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001039 ! DNA laddering assay evidence intersection_of: ECO:0001039 ! DNA laddering assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:26:46Z [Term] id: ECO:0001177 name: RNA dot blot assay evidence used in manual assertion def: "A type of RNA dot blot assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001040 ! RNA dot blot assay evidence intersection_of: ECO:0001040 ! RNA dot blot assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:45:52Z [Term] id: ECO:0001178 name: RNA microarray evidence used in manual assertion def: "A type of RNA microarray evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001041 ! RNA microarray evidence intersection_of: ECO:0001041 ! RNA microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:46:50Z [Term] id: ECO:0001179 name: dominant-negative mutant evidence used in manual assertion def: "A type of dominant-negative mutant evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001042 ! dominant-negative mutant evidence intersection_of: ECO:0001042 ! dominant-negative mutant evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:47:44Z [Term] id: ECO:0001180 name: eTag assay evidence used in manual assertion def: "A type of eTag assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001045 ! eTag assay evidence intersection_of: ECO:0001045 ! eTag assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T16:59:39Z [Term] id: ECO:0001181 name: filter binding assay evidence used in manual assertion def: "A type of filter binding assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001046 ! filter binding assay evidence intersection_of: ECO:0001046 ! filter binding assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:03:28Z [Term] id: ECO:0001182 name: fluorescence in situ hybridization evidence used in manual assertion def: "A type of fluorescence in situ hybridization evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000270 {is_inferred="true"} ! expression evidence used in manual assertion is_a: ECO:0001047 ! fluorescence in situ hybridization evidence intersection_of: ECO:0001047 ! fluorescence in situ hybridization evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:04:20Z [Term] id: ECO:0001183 name: fluorescence resonance energy transfer evidence used in manual assertion def: "A type of fluorescence resonance energy transfer evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001048 ! fluorescence resonance energy transfer evidence intersection_of: ECO:0001048 ! fluorescence resonance energy transfer evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:05:22Z [Term] id: ECO:0001184 name: gel-filtration evidence used in manual assertion def: "A type of gel-filtration evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001049 ! gel-filtration evidence intersection_of: ECO:0001049 ! gel-filtration evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:06:14Z [Term] id: ECO:0001185 name: histochemistry evidence used in manual assertion def: "A type of histochemistry evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000270 {is_inferred="true"} ! expression evidence used in manual assertion is_a: ECO:0001050 ! histochemistry evidence intersection_of: ECO:0001050 ! histochemistry evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:07:19Z [Term] id: ECO:0001186 name: immunocytochemistry evidence used in manual assertion def: "A type of immunocytochemistry evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001053 ! immunocytochemistry evidence intersection_of: ECO:0001053 ! immunocytochemistry evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:12:39Z [Term] id: ECO:0001187 name: histology evidence used in manual assertion def: "A type of histology evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001051 ! histology evidence is_a: ECO:0001256 {is_inferred="true"} ! imaging assay evidence used in manual assertion intersection_of: ECO:0001051 ! histology evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:09:50Z [Term] id: ECO:0001188 name: immunodepletion evidence used in manual assertion def: "A type of immunodepletion evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001054 ! immunodepletion evidence intersection_of: ECO:0001054 ! immunodepletion evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:16:19Z [Term] id: ECO:0001189 name: immunohistochemistry evidence used in manual assertion def: "A type of immunohistochemistry evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001055 ! immunohistochemistry evidence intersection_of: ECO:0001055 ! immunohistochemistry evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:17:22Z [Term] id: ECO:0001190 name: in vitro acetylation assay evidence used in manual assertion def: "A type of in vitro acetylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001057 ! in vitro acetylation assay evidence intersection_of: ECO:0001057 ! in vitro acetylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:19:24Z [Term] id: ECO:0001191 name: in vitro cleavage assay evidence used in manual assertion def: "A type of in vitro cleavage assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001058 ! in vitro cleavage assay evidence intersection_of: ECO:0001058 ! in vitro cleavage assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:21:22Z [Term] id: ECO:0001192 name: in vitro deacetylation assay evidence used in manual assertion def: "A type of in vitro deacetylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001060 ! in vitro deacetylation assay evidence intersection_of: ECO:0001060 ! in vitro deacetylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:23:42Z [Term] id: ECO:0001193 name: in vitro defarnesylation assay evidence used in manual assertion def: "A type of in vitro defarnesylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001061 ! in vitro defarnesylation assay evidence intersection_of: ECO:0001061 ! in vitro defarnesylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:25:45Z [Term] id: ECO:0001194 name: in vitro demethylation assay evidence used in manual assertion def: "A type of in vitro demethylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001062 ! in vitro demethylation assay evidence intersection_of: ECO:0001062 ! in vitro demethylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:27:00Z [Term] id: ECO:0001195 name: in vitro desumoylation assay evidence used in manual assertion def: "A type of in vitro desumoylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001063 ! in vitro desumoylation assay evidence intersection_of: ECO:0001063 ! in vitro desumoylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:29:01Z [Term] id: ECO:0001196 name: in vitro deubiquitination assay evidence used in manual assertion def: "A type of in vitro deubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001059 ! in vitro deubiquitination assay evidence intersection_of: ECO:0001059 ! in vitro deubiquitination assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:29:48Z [Term] id: ECO:0001197 name: in vitro farnesylation assay evidence used in manual assertion def: "A type of in vitro farnesylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001064 ! in vitro farnesylation assay evidence intersection_of: ECO:0001064 ! in vitro farnesylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:30:50Z [Term] id: ECO:0001198 name: in vitro methylation assay evidence used in manual assertion def: "A type of in vitro methylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001065 ! in vitro methylation assay evidence intersection_of: ECO:0001065 ! in vitro methylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:31:54Z [Term] id: ECO:0001199 name: in vitro palmitoylation assay evidence used in manual assertion def: "A type of in vitro palmitoylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001066 ! in vitro palmitoylation assay evidence intersection_of: ECO:0001066 ! in vitro palmitoylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:32:57Z [Term] id: ECO:0001200 name: in vitro phosphatase assay evidence used in manual assertion def: "A type of in vitro phosphatase assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001067 ! in vitro phosphatase assay evidence intersection_of: ECO:0001067 ! in vitro phosphatase assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:34:16Z [Term] id: ECO:0001201 name: in vitro polyADP-ribosylation assay evidence used in manual assertion def: "A type of in vitro polyADP-ribosylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001069 ! in vitro polyADP-ribosylation assay evidence intersection_of: ECO:0001069 ! in vitro polyADP-ribosylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:34:57Z [Term] id: ECO:0001202 name: in vitro protein kinase assay evidence used in manual assertion def: "A type of in vitro protein kinase assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001068 ! in vitro protein kinase assay evidence intersection_of: ECO:0001068 ! in vitro protein kinase assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:39:57Z [Term] id: ECO:0001203 name: in vitro sumoylation assay evidence used in manual assertion def: "A type of in vitro sumoylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001070 ! in vitro sumoylation assay evidence intersection_of: ECO:0001070 ! in vitro sumoylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:41:25Z [Term] id: ECO:0001204 name: in vitro transcription assay evidence used in manual assertion def: "A type of in vitro transcription assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001071 ! in vitro transcription assay evidence intersection_of: ECO:0001071 ! in vitro transcription assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:44:40Z [Term] id: ECO:0001205 name: in vitro translation assay evidence used in manual assertion def: "A type of in vitro translation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001072 ! in vitro translation assay evidence intersection_of: ECO:0001072 ! in vitro translation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:46:30Z [Term] id: ECO:0001206 name: in vitro ubiquitination assay evidence used in manual assertion def: "A type of in vitro ubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001073 ! in vitro ubiquitination assay evidence intersection_of: ECO:0001073 ! in vitro ubiquitination assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:47:18Z [Term] id: ECO:0001207 name: in vivo acetylation assay evidence used in manual assertion def: "A type of in vivo acetylation assay evidence that is used in manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001074 ! in vivo acetylation assay evidence intersection_of: ECO:0001074 ! in vivo acetylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:48:56Z [Term] id: ECO:0001208 name: in vivo cleavage assay evidence used in manual assertion def: "A type of in vivo cleavage assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001075 ! in vivo cleavage assay evidence intersection_of: ECO:0001075 ! in vivo cleavage assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:49:43Z [Term] id: ECO:0001209 name: in vivo deacetylation assay evidence used in manual assertion def: "A type of in vivo deacetylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001076 ! in vivo deacetylation assay evidence intersection_of: ECO:0001076 ! in vivo deacetylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:50:47Z [Term] id: ECO:0001210 name: in vivo defarnesylation assay evidence used in manual assertion def: "A type of in vivo defarnesylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001077 ! in vivo defarnesylation assay evidence intersection_of: ECO:0001077 ! in vivo defarnesylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:52:41Z [Term] id: ECO:0001211 name: in vivo demethylation assay evidence used in manual assertion def: "A type of in vivo demethylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001078 ! in vivo demethylation assay evidence intersection_of: ECO:0001078 ! in vivo demethylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:53:40Z [Term] id: ECO:0001212 name: in vivo desumoylation assay evidence used in manual assertion def: "A type of in vivo desumoylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001080 ! in vivo desumoylation assay evidence intersection_of: ECO:0001080 ! in vivo desumoylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:54:36Z [Term] id: ECO:0001213 name: in vivo deubiquitination assay evidence used in manual assertion def: "A type of in vivo deubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001079 ! in vivo deubiquitination assay evidence intersection_of: ECO:0001079 ! in vivo deubiquitination assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:56:28Z [Term] id: ECO:0001214 name: in vivo farnesylation assay evidence used in manual assertion def: "A type of in vivo farnesylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001081 ! in vivo farnesylation assay evidence intersection_of: ECO:0001081 ! in vivo farnesylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T17:57:18Z [Term] id: ECO:0001215 name: in vivo methylation assay evidence used in manual assertion def: "A type of in vivo methylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001082 ! in vivo methylation assay evidence intersection_of: ECO:0001082 ! in vivo methylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:03:16Z [Term] id: ECO:0001216 name: in vivo palmitoylation assay evidence used in manual assertion def: "A type of in vivo palmitoylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001083 ! in vivo palmitoylation assay evidence intersection_of: ECO:0001083 ! in vivo palmitoylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:04:07Z [Term] id: ECO:0001217 name: in vivo phosphatase assay evidence used in manual assertion def: "A type of in vivo phosphatase assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001084 ! in vivo phosphatase assay evidence intersection_of: ECO:0001084 ! in vivo phosphatase assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:06:08Z [Term] id: ECO:0001218 name: in vivo protein kinase assay evidence used in manual assertion def: "A type of in vivo protein kinase assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001085 ! in vivo protein kinase assay evidence intersection_of: ECO:0001085 ! in vivo protein kinase assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:07:58Z [Term] id: ECO:0001219 name: in vivo sumoylation assay evidence used in manual assertion def: "A type of in vivo sumoylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001086 ! in vivo sumoylation assay evidence intersection_of: ECO:0001086 ! in vivo sumoylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:09:49Z [Term] id: ECO:0001220 name: in vivo transcription assay evidence used in manual assertion def: "A type of in vivo transcription assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001087 ! in vivo transcription assay evidence intersection_of: ECO:0001087 ! in vivo transcription assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:11:20Z [Term] id: ECO:0001221 name: in vivo translation assay evidence used in manual assertion def: "A type of in vivo translation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001088 ! in vivo translation assay evidence intersection_of: ECO:0001088 ! in vivo translation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:42:50Z [Term] id: ECO:0001222 name: in vivo ubiquitination assay evidence used in manual assertion def: "A type of in vivo ubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001089 ! in vivo ubiquitination assay evidence intersection_of: ECO:0001089 ! in vivo ubiquitination assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:44:11Z [Term] id: ECO:0001223 name: induced mutation evidence used in manual assertion def: "A type of induced mutation evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001056 ! induced mutation evidence intersection_of: ECO:0001056 ! induced mutation evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:45:56Z [Term] id: ECO:0001224 name: knockin evidence used in manual assertion def: "A type of knockin evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001090 ! knockin evidence intersection_of: ECO:0001090 ! knockin evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:49:35Z [Term] id: ECO:0001225 name: knockout evidence used in manual assertion def: "A type of knockout evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001091 ! knockout evidence intersection_of: ECO:0001091 ! knockout evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:51:31Z [Term] id: ECO:0001226 name: lipid binding assay evidence used in manual assertion def: "A type of lipid binding assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001092 ! lipid binding assay evidence intersection_of: ECO:0001092 ! lipid binding assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:52:19Z [Term] id: ECO:0001227 name: luminescence-based mammalian interactome mapping assay evidence used in manual assertion def: "A type of LUMIER assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001093 ! luminescence-based mammalian interactome mapping assay evidence intersection_of: ECO:0001093 ! luminescence-based mammalian interactome mapping assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:55:16Z [Term] id: ECO:0001228 name: macroscopy evidence used in manual assertion def: "A type of macroscopy evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001094 ! macroscopy evidence intersection_of: ECO:0001094 ! macroscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:56:21Z [Term] id: ECO:0001229 name: mammalian 2-hybrid assay evidence used in manual assertion def: "A type of mammalian 2-hybrid assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001095 ! mammalian 2-hybrid assay evidence intersection_of: ECO:0001095 ! mammalian 2-hybrid assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:57:25Z [Term] id: ECO:0001230 name: mass spectrometry evidence used in manual assertion def: "A type of mass spectrometry evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001096 ! mass spectrometry evidence intersection_of: ECO:0001096 ! mass spectrometry evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T18:59:50Z [Term] id: ECO:0001231 name: medical imaging evidence used in manual assertion def: "A type of medical imaging evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001097 ! medical imaging evidence is_a: ECO:0001256 {is_inferred="true"} ! imaging assay evidence used in manual assertion intersection_of: ECO:0001097 ! medical imaging evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T19:04:43Z [Term] id: ECO:0001232 name: microscopy evidence used in manual assertion def: "A type of microscopy evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001098 ! microscopy evidence is_a: ECO:0001256 {is_inferred="true"} ! imaging assay evidence used in manual assertion intersection_of: ECO:0001098 ! microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T19:05:37Z [Term] id: ECO:0001233 name: motility wound healing assay evidence used in manual assertion def: "A type of motility wound healing assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001099 ! motility wound healing assay evidence intersection_of: ECO:0001099 ! motility wound healing assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T19:08:13Z [Term] id: ECO:0001234 name: MTS assay evidence used in manual assertion def: "A type of MTS assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001100 ! MTS assay evidence intersection_of: ECO:0001100 ! MTS assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T19:08:51Z [Term] id: ECO:0001235 name: MTT assay evidence used in manual assertion def: "A type of MTT assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001101 ! MTT assay evidence intersection_of: ECO:0001101 ! MTT assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T19:13:17Z [Term] id: ECO:0001236 name: multiplex bead-based immunoassay evidence used in manual assertion def: "A type of multiplex bead-based immunoassay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001102 ! multiplex bead-based immunoassay evidence intersection_of: ECO:0001102 ! multiplex bead-based immunoassay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T19:14:03Z [Term] id: ECO:0001237 name: natural variation mutant evidence used in manual assertion def: "A type of natural variation mutant evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001103 ! natural variation mutant evidence intersection_of: ECO:0001103 ! natural variation mutant evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T19:15:48Z [Term] id: ECO:0001238 name: nuclear magnetic resonance spectroscopy evidence used in manual assertion def: "A type of nuclear magnetic resonance spectroscopy evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001105 ! nuclear magnetic resonance spectroscopy evidence intersection_of: ECO:0001105 ! nuclear magnetic resonance spectroscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T19:18:50Z [Term] id: ECO:0001239 name: nuclear fragmentation evidence used in manual assertion def: "A type of nuclear fragmentation evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001104 ! nuclear fragmentation evidence intersection_of: ECO:0001104 ! nuclear fragmentation evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T19:19:55Z [Term] id: ECO:0001240 name: nuclease protection assay evidence used in manual assertion def: "A type of nuclease protection assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001106 ! nuclease protection assay evidence intersection_of: ECO:0001106 ! nuclease protection assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T19:23:41Z [Term] id: ECO:0001241 name: nucleotide analog incorporation evidence used in manual assertion def: "A type of nucleotide analog incorporation evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001107 ! nucleotide analog incorporation evidence intersection_of: ECO:0001107 ! nucleotide analog incorporation evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T19:25:17Z [Term] id: ECO:0001242 name: phage display evidence used in manual assertion def: "A type of phage display evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001108 ! phage display evidence intersection_of: ECO:0001108 ! phage display evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T19:27:17Z [Term] id: ECO:0001243 name: phosphoamino acid analysis evidence used in manual assertion def: "A type of phosphoamino acid analysis evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001109 ! phosphoamino acid analysis evidence intersection_of: ECO:0001109 ! phosphoamino acid analysis evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T19:28:59Z [Term] id: ECO:0001244 name: peptide affinity enrichment evidence used in manual assertion def: "A type of peptide affinity enrichment evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001110 ! peptide affinity enrichment evidence intersection_of: ECO:0001110 ! peptide affinity enrichment evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T19:30:13Z [Term] id: ECO:0001245 name: peptide array evidence used in manual assertion def: "A type of peptide array evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001112 ! peptide array evidence intersection_of: ECO:0001112 ! peptide array evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:16:45Z [Term] id: ECO:0001246 name: physical examination evidence used in manual assertion def: "A type of physical examination evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001111 ! physical examination evidence intersection_of: ECO:0001111 ! physical examination evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:17:27Z [Term] id: ECO:0001247 name: point mutation evidence used in manual assertion def: "A type of point mutation evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001113 ! point mutation evidence intersection_of: ECO:0001113 ! point mutation evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:18:10Z [Term] id: ECO:0001248 name: propidium iodide staining evidence used in manual assertion def: "A type of propidium iodide staining evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001114 ! propidium iodide staining evidence intersection_of: ECO:0001114 ! propidium iodide staining evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:18:58Z [Term] id: ECO:0001249 name: fluorescence evidence used in manual assertion def: "A type of protein detection by fluorescence evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001115 ! fluorescence evidence intersection_of: ECO:0001115 ! fluorescence evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:19:35Z [Term] id: ECO:0001250 name: protein dot blot assay evidence used in manual assertion def: "A type of protein dot blot assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001116 ! protein dot blot assay evidence intersection_of: ECO:0001116 ! protein dot blot assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:20:30Z [Term] id: ECO:0001251 name: protein microarray evidence used in manual assertion def: "A type of protein microarray evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001117 ! protein microarray evidence intersection_of: ECO:0001117 ! protein microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:21:11Z [Term] id: ECO:0001252 name: protein sequencing assay evidence used in manual assertion def: "A type of protein sequencing assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001118 ! protein sequencing assay evidence intersection_of: ECO:0001118 ! protein sequencing assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:22:08Z [Term] id: ECO:0001253 name: quantitative mass spectrometry evidence used in manual assertion def: "A type of quantitative mass spectrometry evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001119 ! quantitative mass spectrometry evidence is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion intersection_of: ECO:0001119 ! quantitative mass spectrometry evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:23:15Z [Term] id: ECO:0001254 name: radioisotope assay evidence used in manual assertion def: "A type of radioisotope assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001120 ! radioisotope assay evidence intersection_of: ECO:0001120 ! radioisotope assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:27:05Z [Term] id: ECO:0001255 name: radioimmunoassay evidence used in manual assertion def: "A type of radioimmunoassay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001121 ! radioimmunoassay evidence intersection_of: ECO:0001121 ! radioimmunoassay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:28:04Z [Term] id: ECO:0001256 name: imaging assay evidence used in manual assertion def: "A type of imaging assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0000324 ! imaging assay evidence intersection_of: ECO:0000324 ! imaging assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:00Z [Term] id: ECO:0001257 name: restriction fragment detection evidence used in manual assertion def: "A type of restriction fragment detection evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001124 ! restriction fragment detection evidence intersection_of: ECO:0001124 ! restriction fragment detection evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:05Z [Term] id: ECO:0001258 name: spectrophotometry evidence used in manual assertion def: "A type of spectrophotometry evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001126 ! spectrophotometry evidence intersection_of: ECO:0001126 ! spectrophotometry evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:09Z [Term] id: ECO:0001259 name: syngeneic transplantation experiment evidence used in manual assertion def: "A type of syngeneic transplantation experiment evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001128 ! syngeneic transplantation experiment evidence intersection_of: ECO:0001128 ! syngeneic transplantation experiment evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:13Z [Term] id: ECO:0001260 name: xenotransplantation experiment evidence used in manual assertion def: "A type of xenotransplantation experiment evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001137 ! xenotransplantation experiment evidence intersection_of: ECO:0001137 ! xenotransplantation experiment evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:18Z [Term] id: ECO:0001261 name: WST-1 assay evidence used in manual assertion def: "A type of WST-1 assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001136 ! WST-1 assay evidence intersection_of: ECO:0001136 ! WST-1 assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:22Z [Term] id: ECO:0001262 name: wound healing assay evidence used in manual assertion def: "A type of wound healing assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001135 ! wound healing assay evidence intersection_of: ECO:0001135 ! wound healing assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:26Z [Term] id: ECO:0001263 name: urine test evidence used in manual assertion def: "A type of urine test evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001134 ! urine test evidence intersection_of: ECO:0001134 ! urine test evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:30Z [Term] id: ECO:0001264 name: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion def: "A type of TUNEL assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001133 ! terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence intersection_of: ECO:0001133 ! terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:34Z [Term] id: ECO:0001265 name: tryptic phosphopeptide mapping assay evidence used in manual assertion def: "A type of tryptic phosphopeptide mapping assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001132 ! tryptic phosphopeptide mapping assay evidence is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion intersection_of: ECO:0001132 ! tryptic phosphopeptide mapping assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:38Z [Term] id: ECO:0001266 name: transgenic organism evidence used in manual assertion def: "A type of transgenic organism evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001131 ! transgenic organism evidence intersection_of: ECO:0001131 ! transgenic organism evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:41Z [Term] id: ECO:0001267 name: tissue microarray evidence used in manual assertion def: "A type of tissue microarray evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000271 {is_inferred="true"} ! array experiment evidence used in manual assertion is_a: ECO:0001130 ! tissue microarray evidence intersection_of: ECO:0001130 ! tissue microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:45Z [Term] id: ECO:0001268 name: TACE activity assay evidence used in manual assertion def: "A type of TACE activity assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001129 ! TACE activity assay evidence is_a: ECO:0005801 {is_inferred="true"} ! enzyme assay evidence used in manual assertion intersection_of: ECO:0001129 ! TACE activity assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:49Z [Term] id: ECO:0001269 name: surface plasmon resonance evidence used in manual assertion def: "A type of surface plasmon resonance evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001127 ! surface plasmon resonance evidence intersection_of: ECO:0001127 ! surface plasmon resonance evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:54Z [Term] id: ECO:0001270 name: restriction landmark genomic scanning evidence used in manual assertion def: "A type of restriction landmark genome scanning evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000089 ! restriction landmark genomic scanning evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000089 ! restriction landmark genomic scanning evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:29:58Z [Term] id: ECO:0001271 name: resonant mirror biosensor evidence used in manual assertion def: "A type of resonant mirror biosensor evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001123 ! resonant mirror biosensor evidence intersection_of: ECO:0001123 ! resonant mirror biosensor evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T20:30:02Z [Term] id: ECO:0001272 name: high-performance liquid chromatography evidence used in manual assertion def: "A type of high-performance liquid chromatography evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001052 ! high-performance liquid chromatography evidence intersection_of: ECO:0001052 ! high-performance liquid chromatography evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-16T20:49:54Z [Term] id: ECO:0001273 name: ectopic expression evidence used in manual assertion def: "A type of ectopic expression evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000017 ! ectopic expression evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000017 ! ectopic expression evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos creation_date: 2014-03-17T22:34:56Z [Term] id: ECO:0001601 name: protein-binding microarray evidence def: "A type of oligonucleotide microarray evidence that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites." [PMID:22146299] synonym: "PBM evidence" EXACT [] is_a: ECO:0000093 ! oligonucleotide microarray evidence is_a: ECO:0000136 ! nucleic acid binding evidence created_by: swolfish creation_date: 2015-06-05T14:36:00Z [Term] id: ECO:0001603 name: in vivo cell staining evidence def: "A type of in vivo assay evidence in which living cells are stained with a vital stain, i.e. dye that can be used on living cells without causing cell death." [GOC:MAH] synonym: "vital staining evidence" RELATED [GOC:MAH] is_a: ECO:0000178 ! in vivo assay evidence created_by: swolfish creation_date: 2015-06-08T11:51:37Z [Term] id: ECO:0001801 name: alkaline phosphatase reporter gene assay evidence def: "A type of reporter gene assay evidence based on the fusion of selected genes with the phoA gene to express alkaline phosphatase in periplasmic space for protein tracing." [ECO:SW, PMID:11823238] comment: Alkaline phosphatase reporter assay produces a hybrid protein with alkaline phosphatase activity following transportation across the cellular membrane. synonym: "SEAP reporter assay" RELATED [] synonym: "Secreted Embryonic Alkaline Phosphatase reporter assay" RELATED [] is_a: ECO:0000049 ! reporter gene assay evidence created_by: swolfish creation_date: 2015-10-29T15:53:45Z [Term] id: ECO:0001802 name: beta-galactosidase reporter gene assay evidence def: "A type of reporter gene assay evidence based on the fusion of the lacZ gene to a specific promoter for the expression of beta-galactosidase which will appear blue when grown on a X-gal medium." [ECO:SW, PMID:20439410] comment: The assay is often performed using a plasmid borne construction on a lacZ strain. synonym: "beta-gal reporter gene assay" EXACT [] synonym: "lacZ reporter gene assay" RELATED [] is_a: ECO:0000049 ! reporter gene assay evidence created_by: swolfish creation_date: 2015-10-29T15:57:10Z [Term] id: ECO:0001803 name: chloramphenicol acetyltransferase reporter gene assay evidence def: "A type of reporter gene assay evidence based on the fusion of the CAT gene to a specific promoter for the expression of chloramphenicol acetyltransferase which confers resistance to the chloramphenicol antibiotic." [ECO:SW, PMID:1630936] comment: The amount of acetylated chloramphenicol is directly proportional to the amount of CAT enzyme present. synonym: "CAT reporter gene assay" EXACT [PMID:1630936] is_a: ECO:0000049 ! reporter gene assay evidence created_by: swolfish creation_date: 2015-10-29T16:06:42Z [Term] id: ECO:0001804 name: beta-glucuronidase reporter gene assay evidence def: "A type of reporter gene assay evidence where the beta-glucuronidase enzyme from Escherichia coli is used as the reporter to transform non-fluorescent substrates into fluorescents for detection." [ECO:SW] synonym: "GUS reporter gene assay" EXACT [] is_a: ECO:0000049 ! reporter gene assay evidence created_by: swolfish creation_date: 2015-10-29T16:11:02Z [Term] id: ECO:0001805 name: luciferase reporter gene assay evidence def: "A type of reporter gene assay evidence where luciferase, an oxidative enzyme, is used as the reporter to detect a gene product with bioluminescence." [ECO:SW, PMID:17623934] is_a: ECO:0000049 ! reporter gene assay evidence created_by: swolfish creation_date: 2015-10-29T16:15:39Z [Term] id: ECO:0001806 name: chromatin immunoprecipitation- exonuclease evidence def: "A type of chromatin immunoprecipitation evidence that uses gama-exonuclease to digest TF-unbound DNA after ChIP for the identification of transcription factor binding site locations with high-resolution data." [ECO:SW, PMID:25249628] synonym: "ChIP-exo" EXACT [PMID:25249628] is_a: ECO:0000226 ! chromatin immunoprecipitation evidence created_by: swolfish creation_date: 2015-10-29T16:37:36Z [Term] id: ECO:0001807 name: electrophoretic mobility shift assay evidence used in manual assertion def: "A type of electrophoretic mobility shift that is used in a manual assertion." [ECO:SW] is_a: ECO:0000096 ! electrophoretic mobility shift assay evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000096 ! electrophoretic mobility shift assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: swolfish creation_date: 2015-11-03T12:13:17Z [Term] id: ECO:0001808 name: reverse transcription polymerase chain reaction evidence used in manual assertion def: "A type of reverse transcription polymerase chain reaction evidence that is used in a manual assertion." [ECO:SW] is_a: ECO:0000109 ! reverse transcription polymerase chain reaction evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000109 ! reverse transcription polymerase chain reaction evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: swolfish creation_date: 2015-11-03T12:22:46Z [Term] id: ECO:0001809 name: DNA affinity chromatography evidence def: "A type of chromatography evidence where immobilized promoter DNA is labeled and bound to a matrix through which a protein mixture is poured and then eluted to purify specific DNA binding proteins." [ECO:SW, PMID:11694305, PMID:11725488] comment: Biotinylation is commonly used for labeling and beads are commonly used as the matrix. Bound protein will remain attached to the beads. Results can be detected through gel electrophoresis and then sequenced by mass-spectrometry. This technique can be used to demonstrate binding of a purified protein, or to purify the binding protein from crude extract or protein mixture. synonym: "DNA affinity purification" RELATED [] is_a: ECO:0000325 ! chromatography evidence created_by: swolfish creation_date: 2015-11-03T13:48:49Z [Term] id: ECO:0001810 name: DNAse footprinting evidence def: "A type of nucleic acid binding evidence where proteins that have been bound to DNA protect a binding site from enzymatic cleavage with DNAse, thereby detecting protein-DNA interactions." [ECO:SW, PMID:212715, PMID:22194258] comment: In DNAse footingprinting amplified DNA combined with TF and DNAse is electrophoresed for fragment comparison with a non-TF control sample. TF-bound fragments do not appear in the gel. Such fragments can be isolated, purified, and sequenced. This technique is often used to identify the binding motif of a TF resolving the protected region to 50-100 bp which can be further examined for TF-binding sites. synonym: "DNase protection" RELATED [] is_a: ECO:0000136 ! nucleic acid binding evidence created_by: swolfish creation_date: 2015-11-03T13:55:15Z [Term] id: ECO:0001811 name: fluorescence anisotropy evidence def: "A type of fluorescence evidence based on rapid and quantitative analysis of diverse molecular interactions, enzyme activities, and nucleic acid hybridization which uses a fluorophore to measure the binding constants and kinetics of reactions that cause a change in the rotational time of the molecules." [ECO:SW, PMID:20232898] comment: The degree of polarization of a fluorophore is inversely related to its molecular rotation. When the fluorophore is bound to a small molecule, the rate at which it tumbles can decrease significantly from when it is bound tightly to a large protein. If the fluorophore is attached to the larger protein in a binding pair, the difference in polarization between bound and unbound states will be smaller and less accurate. The degree of binding is calculated by using the difference in anisotropy of the partially bound, free, and fully bound states measured by titrating the two binding partners. Fluorescence polarixation assays are homogeneous. synonym: "FA" EXACT [] synonym: "Fluorescence polarization" RELATED [PMCID:3277431, PMID:20232898] synonym: "FP" RELATED [PMCID:3277431, PMID:20232898] is_a: ECO:0001115 ! fluorescence evidence created_by: swolfish creation_date: 2015-11-03T14:01:36Z [Term] id: ECO:0001812 name: genomic systematic evolution of ligands by exponential amplification evidence def: "A type of systematic evolution of ligands by exponential amplification evidence based on a restricted genomic DNA library to identify naturally occurring genomic aptamers and RNA-protein interaction networks with RNA-binding protein as bait and high-throughput sequencing." [ECO:SW, PMID:20541015, PMID:21720957] comment: The reported sequences should always be verified for presence in the genome sequence. synonym: "genomic SELEX" EXACT [PMID:20541015] is_a: ECO:0000293 ! systematic evolution of ligands by exponential amplification evidence created_by: swolfish creation_date: 2015-11-03T14:05:37Z [Term] id: ECO:0001813 name: heteronuclear single quantum coherence spectroscopy evidence def: "A type of nuclear magnetic resonance spectroscopy evidence based on two-dimensional NMR for elucidation of the chemical structure of an isolated or synthesized chemical compound where the characteristic transfer magnetization of a proton to a nitrogen or carbon isotope is monitored by NMR, generating a specific peak in the spectrum." [ECO:SW, PMID:19856946] comment: Chemical shifts in the spectrum indicating binding can be detected from analysis of a protein spectrum in the presence or absence of its cognate DNA binding site. synonym: "HSQC" EXACT [PMID:19856946] is_a: ECO:0001105 ! nuclear magnetic resonance spectroscopy evidence created_by: swolfish creation_date: 2015-11-03T14:11:06Z [Term] id: ECO:0001814 name: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence def: "A type of nucleic acid binding evidence where the synthetic molecule methidiumpropyl-EDTA (MPE) is used to cleave ligand-protected DNA, followed by analysis of the restriction fragments to generate a footprint (i.e. size and location) of small molecule binding sites on the DNA." [ECO:SW, PMID:6225070] synonym: "Methidiumpropyl-EDTA Fe(II) footprinting" RELATED [PMID:6225070] synonym: "MPE Fe(II) footprinting" RELATED [PMID:6225070] synonym: "MPE-EDTA Fe(II) footprinting" RELATED [PMID:6225070] is_a: ECO:0000136 ! nucleic acid binding evidence created_by: swolfish creation_date: 2015-11-03T14:24:04Z [Term] id: ECO:0001815 name: copper-phenanthroline footprinting evidence def: "A type of nucleic acid binding evidence where nucleic acid that has been bound to protein is cleaved with 1,10-phenanthroline-copper complex resulting in a high-resolution footprint of sequence-specific protein-DNA contacts." [ECO:SW, PMID:11691942] synonym: "1,10-Phenanthroline-copper footprinting" EXACT [PMID:1384472] synonym: "OP-Cu Complex" RELATED [PMID:1384472] is_a: ECO:0000136 ! nucleic acid binding evidence created_by: swolfish creation_date: 2015-11-04T08:37:51Z [Term] id: ECO:0001816 name: green fluorescent protein reporter gene assay evidence def: "A type of reporter gene assay evidence based on the fusion of select genes with the green fluorescent protein (GFP) gene for detection with bioluminescence of the gene product when exposed to blue ultraviolet light." [ECO:SW, PMID:11989662] synonym: "GFP promoter fusion" RELATED [] synonym: "GFP reporter gene assay" EXACT [PMID:11989662] synonym: "green fluorescent protein promoter fusion" RELATED [] is_a: ECO:0000049 ! reporter gene assay evidence created_by: swolfish creation_date: 2015-11-04T08:58:42Z [Term] id: ECO:0001817 name: glutathione S-transferase pull-down assay evidence def: "A type of protein binding evidence where a gene is fused with the GST gene and the resulting recombinant bait protein is captured on an immobilized Glutathione affinity ligand and incubated with prey protein to identify and characterize protein-protein interactions." [ECO:SW, PMID:26096507] comment: The recombinant protein can also be eluted following the pull-down and analyzed further with qPCR or sequencing. synonym: "GST pull-down assay" EXACT [PMID:26096507] is_a: ECO:0000024 ! protein binding evidence created_by: swolfish creation_date: 2015-11-04T09:05:56Z [Term] id: ECO:0001818 name: hydroxyl-radical footprinting evidence def: "A type of nucleic acid binding evidence used to identify protein-binding sites on the DNA molecule where DNA that has been bound to protein is digested with hydroxyl radical produced by reduction of hydrogen peroxide with iron (II), followed by separating the cleavage products on a denaturing electrophoresis gel." [ECO:SW, PMID:18546600, PMID:19378159, PMID:3090544] is_a: ECO:0000136 ! nucleic acid binding evidence created_by: swolfish creation_date: 2015-11-04T09:20:26Z [Term] id: ECO:0001819 name: primer extension assay evidence def: "A type of transcript expression level evidence used to determine expression levels of mRNA where a radiolabeled synthetic oligonucleotide primer is annealed to mRNA at the transcription start site of a gene and the mRNA is extended with reverse transcriptase producing cDNA which is electrophoresed on a polyacrylamide gel and autoradiographed, and the band size is proportional to the initial RNA." [ECO:SW, PMID:23378648] comment: The most commonly used radiolabel is 32P. is_a: ECO:0000048 ! transcript expression level evidence created_by: swolfish creation_date: 2015-11-04T09:33:18Z [Term] id: ECO:0001820 name: rapid amplification of cDNA ends polymerase chain reaction evidence def: "A type of polymerase chain reaction evidence where a mRNA primer is used for RT-PCR and terminal deoxynucleotidyl transferase (TdT) is used to add identical nucleotides to the 3' end of the cDNA after which PCR with a different primer and TdT-oligomer are performed in the other sense to obtain the full length sequence of a RNA transcript." [ECO:SW, PMID:17498297] comment: RACE PCR is frequently used to verify transcription start sites relevant to the function of transcription factor binding sites such as repression. synonym: "RACE PCR" EXACT [PMI:17498297] is_a: ECO:0000082 ! polymerase chain reaction evidence created_by: swolfish creation_date: 2015-11-04T09:48:38Z [Term] id: ECO:0001821 name: RNA sequencing assay evidence def: "A type of nucleotide sequencing assay evidence where RNA is extracted from a cell, reverse transcribed to cDNA for a snapshot of an organism's transcriptome with precise measurement of transcript levels and their isoforms." [ECO:SW, PMID:19015660] synonym: "RNA-Seq" EXACT [PMID:23851085] synonym: "Whole Transcriptome Shotgun Sequencing" EXACT [PMID:23851085] synonym: "WTTS" EXACT [] is_a: ECO:0000219 ! nucleotide sequencing assay evidence created_by: swolfish creation_date: 2015-11-04T10:15:57Z [Term] id: ECO:0001822 name: survival rate analysis evidence def: "A type of knockout evidence based on the survival of an organism in a particular environment where a gene for an enzyme or regulator is knocked out and results are used as a natural reporter." [ECO:SW] is_a: ECO:0001091 ! knockout evidence created_by: swolfish creation_date: 2015-11-04T10:48:59Z [Term] id: ECO:0001823 name: x-ray crystallography evidence def: "A type of crystallography evidence where a purified sample at high concentration is crystallised and the crystals are exposed to an x ray beam to obtain three dimensional molecular structure of proteins and biological macromolecules." [ECO:SW, PMID:23135450, PMID:24648090] comment: X-ray crystallography can be used for a crystal used to probe at the tridimensional structure of proteins. In some cases, it is possible to co-crystallize the protein bound to its DNA binding site, providing detail on the particular arrangement of the two components. is_a: ECO:0001034 ! crystallography evidence created_by: swolfish creation_date: 2015-11-04T10:56:22Z [Term] id: ECO:0001824 name: DNA adenine methyltransferase identification evidence def: "A type of affinity evidence used to identify DNA binding sites in eukaryotes where Escherichia coli DNA adenine methyltransferase (Dam) is fused to a transcription factor, co-factor, chromatin-associated protein, or nuclear-associated protein, followed by a methyl-dependent PCR to localize methyltransferase in the region of the binding site." [ECO:SW, PMID:16938559, PMID:17545983, PMID:19588092] comment: The methylation tag is detected with methylation-sensitive restriction enzymes, DpnI and DpnII. synonym: "DamID" EXACT [PMID:19588092] is_a: ECO:0000023 ! affinity evidence created_by: swolfish creation_date: 2015-11-05T15:56:13Z [Term] id: ECO:0001825 name: isothermal titration calorimetry evidence def: "A type of affinity evidence where the absorbed or released heat of a biomolecular binding event is directly measured in a reference cell and sample cell after the addition of a ligand using a microcalorimeter for a complete thermodynamic profile of the molecular interaction." [ECO:SW, PMID:10527727] comment: When ITC is used to study TF, the temperature of two identical cells containing a known concentration of TF is monitored. After the ligand is added to the cells in precisely measured aliquots, the temperature difference in each cell is observed to determine target binding. This value can be used to compute the energetics of the reaction and hence, binding affinity of the TF for the DNA fragment. The thermodynamic profile that is measured includes the values of binding constant (K(a)), stoichiometry (n), and the enthalpy of binding (DeltaH(b)). synonym: "ITC" EXACT [PMID:10527727] is_a: ECO:0000023 ! affinity evidence created_by: swolfish creation_date: 2015-11-05T16:06:20Z [Term] id: ECO:0001826 name: ultraviolet light footprinting evidence def: "A type of nucleic acid binding evidence where DNA is non-specifically fragmented with ultraviolet light while protein-bound regions are protected from UV damage and strand breakage patterns are analyzed by PAGE and sequenced to detect protein-DNA contacts." [ECO:SW, PMID:2842760, PMID:7602584] synonym: "ultraviolet footprinting " RELATED [] synonym: "UV footprinting" RELATED [] is_a: ECO:0000136 ! nucleic acid binding evidence created_by: swolfish creation_date: 2015-11-05T16:13:36Z [Term] id: ECO:0001827 name: premethylation interference footprinting evidence def: "A type of nucleic acid binding evidence used to identify protein binding sites on the DNA molecule where multiple copies of a DNA fragment containing a putative TF-binding site are randomly methylated with dimethyl sulfate (DMS) and cleaved at the methyl group, followed by separating the cleavage products on a denaturing electrophoresis gel." [ECO:SW, PMID:1583685] synonym: "methylation interference footprinting" RELATED [] is_a: ECO:0000136 ! nucleic acid binding evidence created_by: swolfish creation_date: 2015-11-05T16:16:27Z [Term] id: ECO:0001828 name: visual sequence inspection evidence def: "A type of inference from background scientific knowledge used in manual assertion where a visual inspection of promoter region is made to identify putative binding sites." [ECO:SW] is_a: ECO:0000306 ! inference from background scientific knowledge used in manual assertion created_by: swolfish creation_date: 2015-11-10T14:40:16Z [Term] id: ECO:0001829 name: ferric uptake regulator titration assay evidence def: "A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis." [ECO:SW, PMID:10713425, PMID:7642488, PMID:8107138] synonym: "Fluorescence polarization" RELATED [PMID:15689115] synonym: "FURTA" EXACT [PMID:10713425] is_a: ECO:0000049 ! reporter gene assay evidence created_by: swolfish creation_date: 2015-11-10T14:55:36Z [Term] id: ECO:0005004 name: cell viability assay evidence is_a: ECO:0005014 ! in vitro cell based assay evidence created_by: snadendla creation_date: 2015-02-13T12:08:01Z [Term] id: ECO:0005007 name: cell proliferation assay evidence is_a: ECO:0005014 ! in vitro cell based assay evidence created_by: snadendla creation_date: 2015-02-11T16:27:13Z [Term] id: ECO:0005008 name: DNA synthesis cell proliferation assay evidence is_a: ECO:0005007 ! cell proliferation assay evidence created_by: snadendla creation_date: 2015-02-11T16:30:10Z [Term] id: ECO:0005009 name: metabolic cell proliferation assay evidence is_a: ECO:0005007 ! cell proliferation assay evidence created_by: snadendla creation_date: 2015-02-11T16:36:33Z [Term] id: ECO:0005010 name: cell proliferation marker detection assay evidence is_a: ECO:0005007 ! cell proliferation assay evidence created_by: snadendla creation_date: 2015-02-11T16:37:23Z [Term] id: ECO:0005011 name: ATP bioluminescence assay evidence is_a: ECO:0005007 ! cell proliferation assay evidence created_by: snadendla creation_date: 2015-02-11T16:47:30Z [Term] id: ECO:0005012 name: cytotoxicity assay evidence is_a: ECO:0005014 ! in vitro cell based assay evidence created_by: snadendla creation_date: 2015-02-12T11:39:35Z [Term] id: ECO:0005014 name: in vitro cell based assay evidence is_a: ECO:0000181 ! in vitro assay evidence created_by: snadendla creation_date: 2015-02-12T13:26:55Z [Term] id: ECO:0005019 name: staining evidence is_a: ECO:0000002 ! direct assay evidence created_by: snadendla creation_date: 2015-02-19T11:48:57Z [Term] id: ECO:0005021 name: chemotaxis assay evidence is_a: ECO:0005014 ! in vitro cell based assay evidence created_by: snadendla creation_date: 2015-02-19T13:10:20Z [Term] id: ECO:0005027 name: genetic transformation evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: snadendla creation_date: 2015-03-16T15:33:50Z [Term] id: ECO:0005031 name: structure determination evidence is_a: ECO:0000006 ! experimental evidence created_by: snadendla creation_date: 2015-03-20T12:16:17Z [Term] id: ECO:0005033 name: electron microscopy evidence is_a: ECO:0001098 ! microscopy evidence is_a: ECO:0005031 ! structure determination evidence created_by: snadendla creation_date: 2015-03-20T12:21:48Z [Term] id: ECO:0005034 name: apoptotic assay evidence is_a: ECO:0005014 ! in vitro cell based assay evidence created_by: snadendla creation_date: 2015-04-07T21:58:24Z [Term] id: ECO:0005500 name: in vivo polyADP-ribosylation assay evidence def: "A type of in vivo assay evidence where ADP-ribosylated residues in proteins are detected in cells in vivo." [ECO:SN, PMID:2820766, SIB:PG] is_a: ECO:0000178 ! in vivo assay evidence created_by: snadendla creation_date: 2015-06-15T15:35:05Z [Term] id: ECO:0005501 name: in vivo polyADP-ribosylation assay evidence used in manual assertion def: "A type of in vivo polyADP-ribosylation assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0005500 ! in vivo polyADP-ribosylation assay evidence intersection_of: ECO:0005500 ! in vivo polyADP-ribosylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2015-06-15T15:37:23Z [Term] id: ECO:0005502 name: ex vivo assay evidence def: "A type of biological assay evidence derived by studying an organ, tissues, or cells taken from an organism in an external environment with the minimum alteration of natural conditions." [ECO:SN, PMID:16305312, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2305722/pdf/nihms42807.pdf] is_a: ECO:0000094 ! biological assay evidence created_by: snadendla creation_date: 2015-06-26T11:46:15Z [Term] id: ECO:0005503 name: two-dimensional polyacrylamide gel electrophoresis evidence def: "A type of gel electrophoresis evidence that involves one dimension electrophoresis where biomolecules are electrophoresed on a low percentage polyacrylamide gel to be separated in proportion to their mass or isoelectric point, followed by high voltage electrophoresis on a higher percentage gel in the presence of ethidium bromide to alter non-linear molecular shape." [ECO:SW, url:http\://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html] comment: Two-dimensional polyacrylamide gel electrophoresis is commonly employed to establish a proteome for an organism and demonstrate expression changes. synonym: "2D PAGE" EXACT [] synonym: "2D-PAGE" EXACT [] is_a: ECO:0000337 ! gel electrophoresis evidence created_by: snadendla creation_date: 2015-10-01T16:23:20Z [Term] id: ECO:0005504 name: spectrometry evidence is_a: ECO:0000006 ! experimental evidence created_by: snadendla creation_date: 2015-08-14T15:48:08Z [Term] id: ECO:0005505 name: regular expression motif search evidence def: "A type of motif similarity evidence where the motif, a local alignment of sequences, is represented by a regular expression, which captures the variability of nucleotides at each position in a discrete form and can then be used to search genetic sequences by identifying matches to the regular expression." [ECO:SW, PMID:17337630] comment: Regular expression is a computational tool used for determining whether a given genome contains a DNA region resembling a transcription factor binding motif. For example A/T=W indicates A or T may be present in a column of the alignment. (PMID: 20708667). is_a: ECO:0000028 ! motif similarity evidence created_by: snadendla creation_date: 2015-10-09T13:51:52Z [Term] id: ECO:0005506 name: missense mutation evidence is_a: ECO:0001113 ! point mutation evidence created_by: snadendla creation_date: 2015-09-18T16:52:34Z [Term] id: ECO:0005507 name: nonsense mutation evidence is_a: ECO:0001113 ! point mutation evidence created_by: snadendla creation_date: 2015-09-18T16:55:32Z [Term] id: ECO:0005508 name: silent mutation evidence is_a: ECO:0001113 ! point mutation evidence created_by: snadendla creation_date: 2015-09-18T17:01:12Z [Term] id: ECO:0005509 name: insertion mutation evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: snadendla creation_date: 2015-09-18T17:02:03Z [Term] id: ECO:0005510 name: deletion mutation evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: snadendla creation_date: 2015-09-18T17:02:45Z [Term] id: ECO:0005511 name: duplication mutation evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: snadendla creation_date: 2015-09-18T17:03:25Z [Term] id: ECO:0005512 name: frameshift mutation evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: snadendla creation_date: 2015-09-18T17:05:49Z [Term] id: ECO:0005513 name: repeat expansion mutation evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: snadendla creation_date: 2015-09-18T17:06:34Z [Term] id: ECO:0005514 name: splice site mutation evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: snadendla creation_date: 2015-09-18T17:07:29Z [Term] id: ECO:0005515 name: translocation mutation evidence is_a: ECO:0000059 ! experimental phenotypic evidence created_by: snadendla creation_date: 2015-09-18T17:08:01Z [Term] id: ECO:0005516 name: molecule detection assay evidence is_a: ECO:0000006 ! experimental evidence created_by: snadendla creation_date: 2015-09-24T15:54:21Z [Term] id: ECO:0005517 name: protein detection assay evidence is_a: ECO:0005516 ! molecule detection assay evidence created_by: snadendla creation_date: 2015-09-25T14:27:40Z [Term] id: ECO:0005518 name: RNA detection assay evidence is_a: ECO:0005516 ! molecule detection assay evidence created_by: snadendla creation_date: 2015-09-25T14:43:53Z [Term] id: ECO:0005519 name: DNA detection assay evidence is_a: ECO:0005516 ! molecule detection assay evidence created_by: snadendla creation_date: 2015-09-25T19:33:25Z [Term] id: ECO:0005520 name: interferometric reflectance imaging sensor evidence def: "A type of molecule detection assay evidence based on changes in surface-reflected light due to ligand binding on the surface of a semi-transparent substrate to detect proteins, DNA, and other biological material in a multiplexed, high-throughput microarray format without labels." [ECO:SW, PMID:21587155, PMID:24271115] comment: Differences in the optical path lengths between a surface layer and a buried silica layer can be measured very precisely allowing optical height information to be converted to accumulated mass on the surface. For the purpose of TF-binding site identification, IRIS is coupled with DNA-arrays with immobilized potential binding regions exposed to the protein of interest and eluted. Protein-bound fragments exhibit changes in reflectance that can be used to gauge binding quantitatively. synonym: "IRIS" EXACT [] synonym: "Spectral Reflectance Imaging Biosensor" EXACT [] synonym: "SRIB" EXACT [] is_a: ECO:0005516 ! molecule detection assay evidence created_by: snadendla creation_date: 2015-09-28T16:01:01Z [Term] id: ECO:0005521 name: S1 nuclease protection assay evidence def: "A type of nuclease protection assay evidence where RNA is hybridized with complementary DNA probes and exposed to S1 nuclease for unbound RNA degradation after which the intact RNA is run on a gel for probe size determination and RNA identification to detect and map specific RNAs in a complex mixture of total cellular RNA." [ECO:SW, PMID:21390683] is_a: ECO:0001106 ! nuclease protection assay evidence created_by: snadendla creation_date: 2015-09-28T17:16:56Z [Term] id: ECO:0005522 name: DNA dot blot assay evidence is_a: ECO:0005519 ! DNA detection assay evidence created_by: snadendla creation_date: 2015-09-30T10:03:03Z [Term] id: ECO:0005523 name: DNA dot blot assay evidence used in manual assertion def: "A type of DNA dot blot assay evidence that is used in a manual assertion." [ECO:SN, ECO:SW] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0005522 ! DNA dot blot assay evidence intersection_of: ECO:0005522 ! DNA dot blot assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2015-09-30T10:04:00Z [Term] id: ECO:0005524 name: DNA microarray evidence def: "A type of DNA detection assay evidence where complimentary ssDNA is attached to a solid glass, silicon, or plastic surface and is hybridized with a labeled sample of extracted mRNA or amplified cDNA and stimulated with a laser for fluorescence detected with a scanner." [ECO:SW, PMID:17660860] comment: The mRNA extraction is performed in induced and non-induced cells and reverse transcribed with different fluorophore labeling for controls and experiment. Fluorescence is measured at two different wavelengths and the ratio between the two fluorescence intensities corresponds to the induction level. synonym: "DNA chip" RELATED [PMID:10398077] synonym: "DNA-array expression analysis " RELATED [] is_a: ECO:0005519 ! DNA detection assay evidence created_by: snadendla creation_date: 2015-09-30T10:12:48Z [Term] id: ECO:0005525 name: DNA microarray evidence used in manual assertion def: "A type of DNA microarray evidence that is used in a manual assertion." [ECO:SN, ECO:SW] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0005524 ! DNA microarray evidence intersection_of: ECO:0005524 ! DNA microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2015-09-30T10:13:09Z [Term] id: ECO:0005526 name: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence def: "A type of protein detection assay evidence where soft ionization is used for the analysis of biomolecules for mass determination in a three step process of plate preparation, radiation, and ionization with high throughput technology." [ECO:SW, PMID:21964792, PMID:25354905] comment: MALDI-TOF MS is commonly used for protein identification in constructing a proteome where protein fingerprints obtained by microbial cells are compared with a database of reference spectra by means of various algorithms integrated in systems recently made commercially available. synonym: "MALDI-TOF Mass Spectrometry" EXACT [] synonym: "MALDI-TOF-MS" EXACT [] is_a: ECO:0005517 ! protein detection assay evidence created_by: snadendla creation_date: 2015-09-30T10:51:56Z [Term] id: ECO:0005527 name: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion def: "A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in a manual assertion." [ECO:SN, ECO:SW] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence intersection_of: ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2015-09-30T10:58:51Z [Term] id: ECO:0005528 name: site-directed mutagenesis evidence def: "A type of experimental phenotypic evidence based on target-specified mutagenesis for the characterization of various gene and protein interactions, structures, and functions where oligonucleotide primers are used to alter a nucleotide sequence." [ECO:SW, PMID:21204030, PMID:24011050, PMID:3541892] comment: Site-directed mutagenesis is often performed in tandem with EMSA. It is often implemented only in conserved motif positions or serially through all positions of a site. synonym: "site directed mutagenesis" EXACT [] is_a: ECO:0000059 ! experimental phenotypic evidence created_by: snadendla creation_date: 2015-09-30T11:34:41Z [Term] id: ECO:0005529 name: random mutagenesis evidence def: "A type of experimental phenotypic evidence where radiation or a DNA-damaging agent in vivo, or PCR with degenerate primers in vitro are used for non-targeted mutagenesis." [ECO:SW, PMID:15153637, PMID:18265275] comment: Random mutagenesis is not often used in TF-binding site determination; however, it is sometimes used to investigate the effect of mutations in TF binding domains in conjunction with expression assays. Random mutations can also be introduced via chemicals, error-prone PCR (EP-PCR), degenerate oligonucleotides-Pfu(DOP), UV irradiation, mutator strains, nucleotide analogs, or DNA recombination. is_a: ECO:0000059 ! experimental phenotypic evidence created_by: snadendla creation_date: 2015-09-30T11:55:03Z [Term] id: ECO:0005530 name: random mutagenesis evidence used in manual assertion def: "A type of random mutagenesis evidence that is used in a manual assertion." [] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0005529 ! random mutagenesis evidence intersection_of: ECO:0005529 ! random mutagenesis evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2015-09-30T11:57:00Z [Term] id: ECO:0005531 name: motif discovery evidence def: "A type of multiple sequence alignment evidence based on a set of algorithms that infer over-represented motifs from a set of biological sequences returning one or more local multiple sequence alignment defining the inferred motifs." [ECO:SW, PMID:10812473, PMID:16845028, PMID:8211139] comment: Relationship type between sequences (e.g. orthologs from multiple genomes or co-expressed genes in the same genome) is not necessarily assumed. The local multiple sequence alignment is typically ungapped. A common application of motif discovery is to infer the binding motif of a given transcription factor (TF). This can be based on the results of a whole-genome expression analysis, assuming that co-expressed genes are co-regulated by the same TF (PMID: 19892760), from peaks in ChIP-seq experiments with the TF (PMID: 16264194), or from the comparative genomics analysis of the promoter regions of orthologs (PMID: 23032607). is_a: ECO:0005555 ! multiple sequence alignment evidence created_by: snadendla creation_date: 2015-09-30T15:04:45Z [Term] id: ECO:0005532 name: consensus search evidence def: "A type of motif similarity evidence where the motif is represented by a consensus sequence which incorporates the most frequent nucleotide at each position acting as a generic representative for the sequences in the alignment, and is compared to the genetic sequence for a perfect or imperfect match." [ECO:SW, PMID:15130839] comment: This technique is used to identify transcription factor binding sites by searching a bacterial genome for DNA regions resembling a given TF-binding motif. PMID: 17085555. is_a: ECO:0000028 ! motif similarity evidence created_by: snadendla creation_date: 2015-10-09T14:12:54Z [Term] id: ECO:0005533 name: phylogenetic footprinting evidence def: "A type of motif discovery evidence based on a set of algorithms that infer motifs in non-coding DNA sequences from the conservation of functional regulatory sites through evolution at a higher rate than their local surroundings for the identification of an evolutionary 'footprint'." [ECO:SW, PMID:16477324, PMID:3199442] comment: The name derives to experimental footprinting techniques where transcription factor (TF)-binding sites are identified by sequencing the region of DNA protected from biochemical agents by the bound TF. This technique is used to identify or verify transcription factor-binding motifs, most commonly in the Eukaryota (PMID: 11997340). is_a: ECO:0005531 ! motif discovery evidence created_by: snadendla creation_date: 2015-09-30T16:23:02Z [Term] id: ECO:0005534 name: comparative genomics motif search evidence def: "A type of motif similarity evidence based on comparative genomics which uses a search method to scan a set of genomes for instances of a specific motif, and then applies a comparative criterion to analyze the collective strength of these predictions, based on the assumption that functional instances of the motif will be preserved by natural selection and their prediction will therefore be consistent across genomes." [ECO:SW, PMID:10854408] comment: This technique is used to predict transcription factor binding sites in gene promoter regions by identifying conserved DNA regions across multiple genomes. PMID: 22305460. Orthology is a typical comparative criterion used. is_a: ECO:0000028 ! motif similarity evidence created_by: snadendla creation_date: 2015-10-09T14:30:38Z [Term] id: ECO:0005535 name: machine learning prediction of motif instance evidence def: "A type of match to sequence model evidence which captures the application of a broad suite of supervised and unsupervised machine learning algorithms trained on known motif instances and applied to the prediction of novel putative instances of a motif in a set of biological sequences." [ECO:SW, PMID:17117497, PMID:2014171] comment: Machine learning algorithms include, but are not limited to artificial neural networks, random-forest, k-means clustering, hidden Markov models. This suite of methods can be used to identify or verify transcription factor-binding sites in genomic sequences (PMID: 15703297). is_a: ECO:0000202 ! match to sequence model evidence created_by: snadendla creation_date: 2015-10-01T11:58:36Z [Term] id: ECO:0005536 name: position-specific scoring matrix motif search evidence def: "A type of motif similarity evidence where the motif is represented by a position-specific frequency matrix (PSFM) that specifies the frequency of each base at each position of the motif, from which a position-specific scoring matrix (PSSM) can be derived under the framework of a log-likelihood ratio providing a scoring system based on the likelihood of a sequence given the motif, versus a genomic-frequency based null hypothesis." [ECO:SW, PMID:10812473] comment: This technique is used to predict transcription factor binding sites in gene promoter regions by using a position weight matrix approach. (PMID: 15604457). synonym: "PSSM" NARROW [] synonym: "PSSM motif search" EXACT [] is_a: ECO:0000028 ! motif similarity evidence created_by: snadendla creation_date: 2015-10-09T15:04:35Z [Term] id: ECO:0005537 name: xylE reporter gene assay evidence def: "A type of reporter gene assay evidence based on the fusion of the xylE gene to a specific promoter for the expression of catechol 2,3-dioxygenase that converts the colorless catechol substrate to yellow 2-hydroxymuconic semialdehyde for identification of the expression of a particular gene." [ECO:SW, PMID:2592344] is_a: ECO:0000049 ! reporter gene assay evidence created_by: snadendla creation_date: 2015-10-28T15:31:28Z [Term] id: ECO:0005538 name: computationally derived logical inference is_obsolete: true created_by: snadendla creation_date: 2015-11-17T15:15:50Z [Term] id: ECO:0005539 name: computationally derived logical inference used in automatic assertion is_obsolete: true created_by: snadendla creation_date: 2015-11-17T15:17:47Z [Term] id: ECO:0005540 name: computationally derived logical inference from automatic assertion used in automatic assertion is_obsolete: true created_by: snadendla creation_date: 2015-11-17T15:24:42Z [Term] id: ECO:0005541 name: computationally derived logical inference from manual assertion used in automatic assertion is_obsolete: true created_by: snadendla creation_date: 2015-11-17T15:32:03Z [Term] id: ECO:0005542 name: biological system reconstruction evidence by experimental evidence from single species used in manual assertion def: "A type of biological system reconstruction evidence by experimental evidence from single species that is used in a manual assertion." [ECO:SN, PMID:19450514] comment: Processes, pathways and complexes shown by a single experiment should not be annotated with this term but with ECO:0000353 or a child thereof. is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion is_a: ECO:0005553 ! biological system reconstruction evidence by experimental evidence from single species intersection_of: ECO:0005553 ! biological system reconstruction evidence by experimental evidence from single species intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2015-12-16T15:32:41Z [Term] id: ECO:0005543 name: biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion def: "A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in a manual assertion." [ECO:SN, PMID:22232657] comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. The evidence may originate from a combination of several experiments. is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion is_a: ECO:0005552 ! biological system reconstruction evidence by experimental evidence from mixed species intersection_of: ECO:0005552 ! biological system reconstruction evidence by experimental evidence from mixed species intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2015-12-16T15:45:01Z [Term] id: ECO:0005544 name: biological system reconstruction evidence based on orthology evidence used in manual assertion def: "A type of biological system reconstruction evidence based on orthology evidence that is used in a manual assertion." [ECO:SN] comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. is_a: ECO:0005545 ! biological system reconstruction evidence based on orthology evidence is_a: ECO:0005610 ! biological system reconstruction evidence based on homology evidence used in manual assertion intersection_of: ECO:0005545 ! biological system reconstruction evidence based on orthology evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2015-12-18T16:20:49Z [Term] id: ECO:0005545 name: biological system reconstruction evidence based on orthology evidence def: "A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by orthology from an existing experimentally supported model to a process, pathway or complex in another species." [ECO:SN, PMID:15660128] comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. is_a: ECO:0005549 ! biological system reconstruction evidence based on homology evidence created_by: snadendla creation_date: 2015-12-16T15:57:37Z [Term] id: ECO:0005546 name: biological system reconstruction evidence based on paralogy evidence used in manual assertion def: "A type of biological system reconstruction evidence based on paralogy evidence that is used in a manual assertion." [ECO:SN, PMID:15660128] comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. is_a: ECO:0005550 ! biological system reconstruction evidence based on paralogy evidence is_a: ECO:0005610 ! biological system reconstruction evidence based on homology evidence used in manual assertion intersection_of: ECO:0005550 ! biological system reconstruction evidence based on paralogy evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2015-12-16T16:05:15Z [Term] id: ECO:0005547 name: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion def: "A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in a manual assertion." [ECO:SN, PMID:17876790] comment: Functional studies or ligand binding evidence are often used for the reconstruction of biological systems. It does not provide physical interaction evidence but uses proxies such a ligand binding evidences to infer the presence or absence of the complex components. is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion is_a: ECO:0005548 ! biological system reconstruction evidence based on inference from background scientific knowledge intersection_of: ECO:0005548 ! biological system reconstruction evidence based on inference from background scientific knowledge intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2015-12-16T16:07:42Z [Term] id: ECO:0005548 name: biological system reconstruction evidence based on inference from background scientific knowledge def: "A type of biological system reconstruction based solely on background scientific knowledge of that biological system." [ECO:SN, PMID:17876790] comment: The available knowledge is usually a combination of partial or weak experimental evidence where either some components are missing or no physical interaction evidence can be found but the system is inferred by similarity to related systems in taxonomically-disparate organisms with experimental evidence. Functional studies or ligand binding evidence are often used for the reconstruction of biological systems. It does not provide physical interaction evidence but uses proxies such a ligand binding evidences to infer the presence or absence of the complex components. is_a: ECO:0000088 ! biological system reconstruction evidence created_by: snadendla creation_date: 2015-12-18T15:32:15Z [Term] id: ECO:0005549 name: biological system reconstruction evidence based on homology evidence def: "A type of biological system reconstruction where the evidence is inferred by homology based on conservation of sequence, function, and composition from an existing experimentally supported model to a process, pathway, or complex." [ECO:SN, PMID:15660128] comment: Inference may be based on paralogy and/or orthology of the genome-encoded components and is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments in the same or another species. is_a: ECO:0000088 ! biological system reconstruction evidence created_by: snadendla creation_date: 2015-12-18T15:49:46Z [Term] id: ECO:0005550 name: biological system reconstruction evidence based on paralogy evidence def: "A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by paralogy from an existing experimentally supported model to a process, pathway, or complex in the same species." [ECO:SN, PMID:15660128] comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. is_a: ECO:0005549 ! biological system reconstruction evidence based on homology evidence created_by: snadendla creation_date: 2015-12-18T16:24:08Z [Term] id: ECO:0005551 name: biological system reconstruction evidence by experimental evidence def: "A type of biological system reconstruction evidence that uses experimental evidence as support." [ECO:SN, PMID:19450514, PMID:22232657] is_a: ECO:0000088 ! biological system reconstruction evidence created_by: snadendla creation_date: 2015-12-18T16:34:13Z [Term] id: ECO:0005552 name: biological system reconstruction evidence by experimental evidence from mixed species def: "A type of biological system reconstruction evidence where the experimental evidence is derived by using a mix of species used in the same experiment." [ECO:SN, PMID:22232657] comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. The evidence may originate from a combination of several experiments. is_a: ECO:0005551 ! biological system reconstruction evidence by experimental evidence created_by: snadendla creation_date: 2015-12-18T16:35:13Z [Term] id: ECO:0005553 name: biological system reconstruction evidence by experimental evidence from single species def: "A type of biological system reconstruction where the experimental evidence is derived by using multiple experiments in a single species." [ECO:SN] comment: Processes, pathways and complexes shown by a single experiment should not be annotated with this term but with ECO:0000353 or a child thereof. is_a: ECO:0005551 ! biological system reconstruction evidence by experimental evidence created_by: snadendla creation_date: 2015-12-18T16:36:44Z [Term] id: ECO:0005554 name: pairwise sequence alignment evidence def: "A type of sequence alignment evidence where two sequences are aligned to identify regions of similarity that may indicate functional, structural and/or evolutionary relationships between two biological sequences (protein or nucleic acid)." [ECO:SN, url:http\://www.ebi.ac.uk/Tools/psa/] is_a: ECO:0000200 ! sequence alignment evidence created_by: snadendla creation_date: 2016-02-08T14:30:46Z [Term] id: ECO:0005555 name: multiple sequence alignment evidence def: "A type of sequence alignment evidence where three or more biological sequences (protein or nucleic acid) of similar length are aligned to infer homology and the evolutionary relationships between the sequences studied." [ECO:SN, url:http\://www.ebi.ac.uk/Tools/msa/] is_a: ECO:0000200 ! sequence alignment evidence created_by: snadendla creation_date: 2016-02-08T14:31:24Z [Term] id: ECO:0005556 name: multiple sequence alignment evidence used in manual assertion def: "A type of multiple sequence alignment evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion is_a: ECO:0005555 ! multiple sequence alignment evidence intersection_of: ECO:0005555 ! multiple sequence alignment evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-02-08T14:59:18Z [Term] id: ECO:0005557 name: multiple sequence alignment evidence used in automatic assertion def: "A type of multiple sequence alignment evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000248 {is_inferred="true"} ! sequence alignment evidence used in automatic assertion is_a: ECO:0005555 ! multiple sequence alignment evidence intersection_of: ECO:0005555 ! multiple sequence alignment evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-02-08T15:00:50Z [Term] id: ECO:0005558 name: motif discovery evidence used in manual assertion def: "A type of motif discovery evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0005531 ! motif discovery evidence is_a: ECO:0005556 {is_inferred="true"} ! multiple sequence alignment evidence used in manual assertion intersection_of: ECO:0005531 ! motif discovery evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-02-08T15:07:47Z [Term] id: ECO:0005559 name: motif discovery evidence used in automatic assertion def: "A type of motif discovery evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0005531 ! motif discovery evidence is_a: ECO:0005557 {is_inferred="true"} ! multiple sequence alignment evidence used in automatic assertion intersection_of: ECO:0005531 ! motif discovery evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-02-08T15:08:19Z [Term] id: ECO:0005560 name: pairwise sequence alignment evidence used in manual assertion def: "A type of pairwise sequence alignment evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion is_a: ECO:0005554 ! pairwise sequence alignment evidence intersection_of: ECO:0005554 ! pairwise sequence alignment evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-02-08T15:17:05Z [Term] id: ECO:0005561 name: pairwise sequence alignment evidence used in automatic assertion def: "A type of pairwise sequence alignment evidence that is used in an automatic evidence." [ECO:SN] is_a: ECO:0000248 {is_inferred="true"} ! sequence alignment evidence used in automatic assertion is_a: ECO:0005554 ! pairwise sequence alignment evidence intersection_of: ECO:0005554 ! pairwise sequence alignment evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-02-08T15:17:52Z [Term] id: ECO:0005562 name: in gel protein kinase assay evidence is_a: ECO:0000181 ! in vitro assay evidence created_by: snadendla creation_date: 2016-02-09T12:05:29Z [Term] id: ECO:0005563 name: macroscopic current trace evidence def: "A type of electrophysiology assay evidence that involves recording trace of the current (pA) going through the analyzed ion channels determined from voltage clamp current recording." [ECO:SN, PMID:17938230, url:http\://www.utdallas.edu/~tres/microelectrode/microelectrodes_ch03.pdf] is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-09T12:40:03Z [Term] id: ECO:0005564 name: current density evidence def: "A type of electrophysiology assay evidence that involves measuring the amount of current (pA) going through the ion channels per unit of membrane." [ECO:SN, url:http\://circres.ahajournals.org/content/102/11/1298.full, url:http\://www.bem.fi/book/04/04.htm] is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-09T12:43:07Z [Term] id: ECO:0005565 name: single channel conductance evidence is_obsolete: true created_by: snadendla creation_date: 2016-02-09T12:45:42Z [Term] id: ECO:0005566 name: sustained current evidence def: "A type of electrophysiology assay evidence that involves the measurement of the current that persists after the fast and slow inactivation of the analyzed channels." [ECO:SN, url:http\://www.nature.com/ncomms/journal/v3/n2/full/ncomms1717.html] is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-09T12:46:54Z [Term] id: ECO:0005567 name: steady state activation curve evidence is_obsolete: true created_by: snadendla creation_date: 2016-02-09T12:48:17Z [Term] id: ECO:0005568 name: steady state inactivation curve evidence is_obsolete: true created_by: snadendla creation_date: 2016-02-09T12:50:45Z [Term] id: ECO:0005569 name: window current trace evidence is_obsolete: true created_by: snadendla creation_date: 2016-02-09T12:57:16Z [Term] id: ECO:0005570 name: use dependence of inactivation evidence def: "A type of electrophysiology assay evidence in which the amount of current remaining after a series of depolarization at different frequencies described as the normalized current related to the time course." [ECO:SN, url:http\://circres.ahajournals.org/content/89/8/700.full] is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-09T12:58:18Z [Term] id: ECO:0005571 name: current clamp voltage recording evidence def: "A type of electrophysiology assay evidence that involves recording the change in membrane potential caused by applying specific current to the cell." [ECO:SN, url:http\://www.nature.com/nprot/journal/v4/n8/full/nprot.2009.91.html] xref: birnlex:2278 "current clamp voltage recording protocol" is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-12T13:58:32Z [Term] id: ECO:0005572 name: whole-cell voltage clamp recording evidence def: "A type of electrophysiology assay evidence that involves direct measurement of ionic current across a membrane while controlling the membrane potential by maintaining the voltage." [ECO:SN, url:http\://circres.ahajournals.org/content/72/1/91.full.pdf] xref: birnlex:2281 "whole-cell voltage clamp recording protocol" is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-12T14:06:40Z [Term] id: ECO:0005573 name: cell-attached single-channel recording evidence def: "A type of electrophysiology assay evidence that involves isolation of a patch of membrane electrically from the external solution and to record current flowing into the patch and this is achieved by pressing a fire-polished glass pipette, which has been filled with a suitable electrolyte solution, against the surface of a cell and applying light suction." [ECO:SN, url:http\://www.utdallas.edu/~tres/microelectrode/microelectrodes_ch04.pdf] xref: birnlex:2283 "cell-attached single-channel recording protocol" is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-12T14:07:39Z [Term] id: ECO:0005574 name: cell-detached inside-out single-channel recording evidence def: "A type of electrophysiology assay evidence that involves study of individual ion channels by gently pulling the patch of membrane away from the cell, and the patch remains attached to the pipette with its cytoplasmic surface exposed to the bathing solution." [ECO:SN, url:http\://www.acnp.org/g4/GN401000005/CH005.html] xref: birnlex:2286 "cell-detached inside-out single-channel recording protocol" is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-12T14:16:53Z [Term] id: ECO:0005575 name: reconstituted bilayer single-channel patch recording evidence def: "A type of electrophysiology assay evidence that involves ion channel analysis by incorporating the ion channels into liposomes suitable for patch-clamping which allows isolation and study of a specific population of channels into that bilayer membrane." [ECO:SN, url:http\://nanion.de/images/stories/papers/Bilayers_lab-on-a-chip08.pdf] xref: birnlex:2287 "reconstituted bilayer single-channel patch recording protocol" is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-12T14:17:46Z [Term] id: ECO:0005576 name: voltage clamp current recording evidence def: "A type of electrophysiology assay evidence that involves recording membrane potential by controlling the membrane voltage and measuring the transmembrane current required to maintain that voltage." [ECO:SN, url:http\://neurobio.drexelmed.edu/GaoWeb/Resources/Recording%20_Techniques.pdf, url:http\://www.acnp.org/g4/GN401000005/CH005.html] xref: birnlex:2279 "voltage clamp current recording protocol" is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-12T14:18:36Z [Term] id: ECO:0005577 name: electroencephalography recording evidence def: "A type of electrophysiology assay evidence that involves recording the electrical activity of the brain by means of electrodes placed on the surface of the head." [ECO:SN, url:http\://www.jove.com/science-education/5420/electro-encephalography-eeg] xref: birnlex:2293 "electroencephalography recording protocol" is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-12T14:19:21Z [Term] id: ECO:0005578 name: super-resolution microscopy evidence def: "A type of microscopy evidence where the images are taken with a higher resolution than the diffraction limit that help in studying nanoscopic sub-cellular structures." [ECO:SN, url:http\://journal.frontiersin.org/article/10.3389/fncel.2015.00007/full] is_a: ECO:0001098 ! microscopy evidence created_by: snadendla creation_date: 2016-02-12T15:20:47Z [Term] id: ECO:0005579 name: immunogold labelling evidence used in manual assertion def: "A type of immunogold labelling evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000090 ! immunogold labelling evidence is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion intersection_of: ECO:0000090 ! immunogold labelling evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-02-12T15:38:03Z [Term] id: ECO:0005580 name: flow cytometry evidence used in manual assertion def: "A type of flow cytometry evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000268 ! flow cytometry evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000268 ! flow cytometry evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-02-12T16:04:23Z [Term] id: ECO:0005581 name: enzyme-linked immunoabsorbent assay evidence used in manual assertion def: "A type of enzyme-linked immunoabsorbent assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000267 ! enzyme-linked immunoabsorbent assay evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000267 ! enzyme-linked immunoabsorbent assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-02-12T16:23:38Z [Term] id: ECO:0005582 name: cell-detached outside-out single-channel recording evidence def: "A type of electrophysiology assay evidence where a part of cellular membrane is detached from the cell and whose external face is exposed to the extracellular medium which allows isolating and studying a single or at least a small number of channels into that fragment (patch)." [ECO:SN, url:http\://www.nature.com/nprot/journal/v2/n11/full/nprot.2007.403.html] is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-03-02T10:47:25Z [Term] id: ECO:0005583 name: cut-open oocyte voltage clamp recording evidence def: "A type of electrophysiology assay evidence that involves inserting the oocyte in a chamber that separates the surface into 3 regions where the top portion of the oocyte membrane is the region that is clamped from which currents are actually recorded while the bottom portion is the region of the oocyte that is cut-open, making it possible to inject current intracellularly through a low resistance pathway." [ECO:SN, PMID:9711615] is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-03-02T11:29:32Z [Term] id: ECO:0005584 name: macropatch voltage clamp recording evidence def: "A type of electrophysiology assay evidence where recording is done on a fragment of the cell membrane (attached to or detached from the cell) using a large tip diameter pipette that allows isolating and studying a small number of channels into that fragment (patch)." [ECO:SN, url:http\://jxb.oxfordjournals.org/content/50/Special_Issue/1037.full.pdf] is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-03-02T11:33:48Z [Term] id: ECO:0005585 name: high throughput mass spectrometry evidence def: "A type of mass spectrometry evidence where mass-to-charge ratio (m/z) of gas-phase ions is measured which is used to identify and quantify molecules in complex solutions within the framework of high throughput studies." [ECO:SN, PMID:20805795] comment: There are significant differences in data analysis and data evaluation if to compare a HTP mass-spec and a regular mass-spec. The regular mass spec method is quite a reliable technique for identifying a protein molecular weight and analysing its post-modifications, other mass-spec applications might require the additional quality controls and more stringent data evaluation. More complex sample mixture is processed, more quality questions should be addressed, such as a sample contamination and sensitivity of the mass spectrometer while using HTP mass-spec.\nFor HTP mass-spec, the analysis of the small data sets allows to manually assign the spectra to proteins or peptides, but for large data sets only the statistical tools can be used instead. is_a: ECO:0001096 ! mass spectrometry evidence created_by: snadendla creation_date: 2016-03-02T12:33:07Z [Term] id: ECO:0005586 name: high throughput mass spectrometry evidence used in manual assertion def: "A type of high throughput mass spectrometry evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0005585 ! high throughput mass spectrometry evidence created_by: snadendla creation_date: 2016-03-02T12:50:02Z [Term] id: ECO:0005587 name: confocal microscopy evidence def: "A type of microscopy evidence where sharp images are taken by excluding most of the light from the specimen that is not from the microscope's focal plane, thus allowing better contrast and less haze, which makes it possible to build three-dimensional reconstructions of a volume of the specimen by assembling a series of thin slices taken along the vertical axis." [ECO:SN, url:http\://www.physics.emory.edu/faculty/weeks//lab/papers/ebbe05.pdf] is_a: ECO:0001098 ! microscopy evidence created_by: snadendla creation_date: 2016-03-17T12:29:45Z [Term] id: ECO:0005588 name: wide-field microscopy evidence def: "A type of microscopy evidence where imaging of a thin cell or a tissue specimen is performed by full aperture emission of light gathered by the microscope's objective, which maximizes the recorded signal and simultaneously minimizes the required exposure times." [ECO:SN, url:http\://jcb.rupress.org/content/172/1/9.full, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048960/, url:http\://zeiss-campus.magnet.fsu.edu/articles/livecellimaging/techniques.html] is_a: ECO:0001098 ! microscopy evidence created_by: snadendla creation_date: 2016-03-17T12:30:27Z [Term] id: ECO:0005589 name: confocal microscopy evidence used in manual assertion def: "A type confocal microscopy evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0005587 ! confocal microscopy evidence intersection_of: ECO:0005587 ! confocal microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-03-17T12:32:24Z [Term] id: ECO:0005590 name: wide-field microscopy evidence used in manual assertion def: "A type of wide-field microscopy evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0005588 ! wide-field microscopy evidence intersection_of: ECO:0005588 ! wide-field microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-03-17T12:34:42Z [Term] id: ECO:0005591 name: immunogold labelling electron microscopy assay evidence def: "A type of immunogold labelling evidence where electron microscopy technique is used to detect the location of the antibodies labeled with colloidal gold particles." [ECO:SN, PMID:4110101] is_a: ECO:0000090 ! immunogold labelling evidence is_a: ECO:0005033 ! electron microscopy evidence created_by: snadendla creation_date: 2016-03-17T15:43:00Z [Term] id: ECO:0005592 name: immunogold labelling electron microscopy assay evidence used in manual assertion def: "A type of immunogold labelling electron microscopy assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0005579 {is_inferred="true"} ! immunogold labelling evidence used in manual assertion is_a: ECO:0005591 ! immunogold labelling electron microscopy assay evidence is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion intersection_of: ECO:0005591 ! immunogold labelling electron microscopy assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-03-17T16:39:37Z [Term] id: ECO:0005593 name: immunodetection assay evidence def: "A type of direct assay evidence that involves the use of antibodies to detect biomolecules." [ECO:SN] is_a: ECO:0000002 ! direct assay evidence created_by: snadendla creation_date: 2016-03-17T17:07:33Z [Term] id: ECO:0005594 name: immunoperoxidase immunolocalization evidence def: "A type of immunolocalization evidence involving enzymatic detection of a peroxidase tagged antibody." [ECO:SN] is_a: ECO:0000087 ! immunolocalization evidence created_by: snadendla creation_date: 2016-03-17T17:11:34Z [Term] id: ECO:0005595 name: immunoperoxidase immunolocalization evidence used in manual assertion def: "A type of immunoperoxidase immunolocalization evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0005594 ! immunoperoxidase immunolocalization evidence intersection_of: ECO:0005594 ! immunoperoxidase immunolocalization evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-03-17T17:14:05Z [Term] id: ECO:0005596 name: immunoperoxidase immunolocalization electron microscopy evidence def: "A type of immunoperoxidase immunolocalization evidence where electron microscopy technique is used for enzymatic detection of a peroxidase tagged antibody." [ECO:SN] synonym: "Immunoperoxidase labelling electron microscopy" EXACT [] is_a: ECO:0005033 ! electron microscopy evidence is_a: ECO:0005594 ! immunoperoxidase immunolocalization evidence created_by: snadendla creation_date: 2016-03-17T17:16:38Z [Term] id: ECO:0005597 name: immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion def: "A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0005595 {is_inferred="true"} ! immunoperoxidase immunolocalization evidence used in manual assertion is_a: ECO:0005596 ! immunoperoxidase immunolocalization electron microscopy evidence is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion intersection_of: ECO:0005596 ! immunoperoxidase immunolocalization electron microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-03-17T17:23:56Z [Term] id: ECO:0005598 name: wide-field fluorescence microscopy evidence def: "A type of wide-field microscopy evidence where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter." [ECO:SN, url:http\://www.bristol.ac.uk/synaptic/research/techniques/widefield.html] comment: The optical filter is used to select the wavelength of excitation light. The detection camera is typically a CCD camera. Both topographical and dynamic information of a sample are obtained. is_a: ECO:0005588 ! wide-field microscopy evidence created_by: snadendla creation_date: 2016-03-18T11:53:56Z [Term] id: ECO:0005599 name: immunofluorescence wide-field microscopy evidence def: "A type of wide-field fluorescence microscopy evidence where fluorescently tagged antibodies are imaged that are used to bind to their antigens." [ECO:SN, url:http\://journals.plos.org/plosone/article?id=10.1371/journal.pone.0057135] synonym: "wide-field epifluorescence microscopy" EXACT [] is_a: ECO:0000007 ! immunofluorescence evidence is_a: ECO:0005598 ! wide-field fluorescence microscopy evidence created_by: snadendla creation_date: 2016-03-18T11:55:40Z [Term] id: ECO:0005600 name: immunofluorescence confocal microscopy evidence def: "A type of confocal microscopy evidence where light passes through immunofluorescent-stained tissue sections or cells and then the emitted light passes through the dichroic mirror and is focused onto the pinhole for subsequent detection." [ECO:SN, url:http\://www.physics.emory.edu/faculty/weeks/confocal/, url:https\://www.hycultbiotech.com/media/wysiwyg/protocol_Immunofluorescence.pdf] comment: This allows visualization of sub cellular distribution of biomolecules of interest. is_a: ECO:0000007 ! immunofluorescence evidence is_a: ECO:0005587 ! confocal microscopy evidence created_by: snadendla creation_date: 2016-03-18T12:48:42Z [Term] id: ECO:0005601 name: immunofluorescence confocal microscopy evidence used in manual assertion def: "A type of immunofluorescence confocal microscopy evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0005589 {is_inferred="true"} ! confocal microscopy evidence used in manual assertion is_a: ECO:0005600 ! immunofluorescence confocal microscopy evidence is_a: ECO:0005804 {is_inferred="true"} ! immunofluorescence evidence used in manual assertion intersection_of: ECO:0005600 ! immunofluorescence confocal microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-03-18T12:52:04Z [Term] id: ECO:0005602 name: protein expression level evidence based on western blot def: "A type of protein expression level evidence where the level of expression of a protein is determined in a cell or tissue extract by western blot technique." [ECO:SN] is_a: ECO:0000046 ! protein expression level evidence is_a: ECO:0000112 ! western blot evidence created_by: snadendla creation_date: 2016-03-18T13:02:29Z [Term] id: ECO:0005603 name: protein mass spectrometry evidence def: "A type of mass spectrometry evidence where one or more protein analyses are performed." [ECO:SN] is_a: ECO:0000039 ! protein assay evidence is_a: ECO:0001096 ! mass spectrometry evidence created_by: snadendla creation_date: 2016-03-18T13:11:37Z [Term] id: ECO:0005604 name: cross-streak test evidence def: "A type of experimental evidence where the investigated organism or a potential antagonist is inoculated so as to grow along the diameter line of the agar medium in a Petri dish, then, potentially susceptible microbes (usually bacteria) are streaked perpendicularly to the growth line and inhibition of growth of a test microbe in the vicinity of that line suggests that the antagonist secretes one or more diffusible antibiotics." [ECO:SN, url:www.jstor.org/stable/3792785] is_a: ECO:0000006 ! experimental evidence created_by: snadendla creation_date: 2016-03-29T15:40:54Z [Term] id: ECO:0005605 name: disk diffusion test evidence def: "A type of experimental evidence where sensitivity/resistance to an antimicrobial agent is tested by impregnating small filter disks with a known concentration of antimicrobial agent which is then placed on a Mueller-Hinton agar plate inoculated with the test organism." [ECO:SN, url:http\://www.cdc.gov/nczved/resources/cholera/ch9.pdf] synonym: "zone of inhibition evidence" EXACT [] is_a: ECO:0000006 ! experimental evidence created_by: snadendla creation_date: 2016-03-29T16:02:22Z [Term] id: ECO:0005606 name: cell transfection experiment evidence def: "A type of experimental evidence where a foreign genetic material is introduced into the cell for study of gene function and regulation and protein function." [ECO:SN, url:http\://ch.promega.com/resources/product-guides-and-selectors/protocols-and-applications-guide/transfection/, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911531/] is_a: ECO:0000006 ! experimental evidence created_by: snadendla creation_date: 2016-03-29T16:59:17Z [Term] id: ECO:0005607 name: tethered cell assay evidence def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined by observing the rotational behavior of the organism tethered to a coverslip by their flagella." [ECO:SN, PMID:1103143] is_a: ECO:0005021 ! chemotaxis assay evidence created_by: snadendla creation_date: 2016-03-31T15:51:10Z [Term] id: ECO:0005608 name: tumble frequency assay evidence def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined by measuring chemotaxis in response to sudden concentration changes from one uniform spatial environment to another." [ECO:SN, PMID:4576832] synonym: "Temporal assay" EXACT [] is_a: ECO:0005021 ! chemotaxis assay evidence created_by: snadendla creation_date: 2016-03-31T16:42:35Z [Term] id: ECO:0005609 name: capillary assay evidence def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined (typically bacteria or archaea) by measuring the rate of accumulation of the organism into a capillary." [ECO:SN, PMID:4632978] comment: To determine the presence of positive chemotaxis, an attractant is placed in the capillary and the presence of positive chemotaxis is determined based on the amount of organism that accumulated in the capillary.\nTo determine the presence of negative chemotaxis, a repellent is added to the organismal suspension (but not in the capillary) and the organisms that fled into the capillary are measured. is_a: ECO:0005021 ! chemotaxis assay evidence created_by: snadendla creation_date: 2016-03-31T16:45:29Z [Term] id: ECO:0005610 name: biological system reconstruction evidence based on homology evidence used in manual assertion def: "A type of biological system reconstruction evidence based on homology evidence that is used in a manual assertion." [ECO:SN] comment: Inference may be based on paralogy and/or orthology of the genome-encoded components and is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments in the same or another species. is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion is_a: ECO:0005549 ! biological system reconstruction evidence based on homology evidence intersection_of: ECO:0005549 ! biological system reconstruction evidence based on homology evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-03-31T17:00:03Z [Term] id: ECO:0005611 name: inference from experimental data evidence def: "A type of direct assay evidence used in manual assertion where phenotype or disease association is made with the gene because of involvement in the molecular mechanism of disease or involvement in a pathway known to contribute to a disease." [ECO:SN] synonym: "IED" EXACT [] synonym: "inferred from experimental data" EXACT [] is_a: ECO:0000314 ! direct assay evidence used in manual assertion created_by: snadendla creation_date: 2016-04-12T16:55:16Z [Term] id: ECO:0005612 name: inference from phenotype manipulation evidence def: "A type of mutant phenotype evidence used in manual assertion where variations or changes, such as mutations or abnormal levels of the product(s) of a single gene of interest, including non-mutational changes such as inhibition with antibodies or other inhibitors are determined." [ECO:SN] comment: Changes can include any gene mutation/knockout, over-expression or ectopic expression of wild-type or mutant genes, anti-sense experiments, RNAi experiments, specific protein inhibitors. synonym: "inferred from phenotype manipulation" EXACT [] synonym: "IPM" EXACT [] is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion created_by: snadendla creation_date: 2016-04-12T17:09:43Z [Term] id: ECO:0005613 name: inference by association of genotype from phenotype def: "A type of natural variation mutant evidence used in manual assertion where alleles are directly linked to phenotype and Mendelian diseases as a result of various experiments." [ECO:SN] comment: Used for 1) polymorphism or segregation of genetic markers (SNPs, mutations, RFLPs, microsatellites); 2) polymorphism or segregation of physical markers (FISH, centromeric or heterochromatic regions, chromosomal banding patterns); 3) detection of polymorphisms in inbred stock. synonym: "IAGP" EXACT [] synonym: "inferred by association of genotype from phenotype" EXACT [] is_a: ECO:0001237 ! natural variation mutant evidence used in manual assertion created_by: snadendla creation_date: 2016-04-12T17:24:00Z [Term] id: ECO:0005614 name: two-dimensional polyacrylamide gel electrophoresis evidence used in manual assertion def: "A type of two-dimensional polyacrylamide gel electrophoresis evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0005503 ! two-dimensional polyacrylamide gel electrophoresis evidence intersection_of: ECO:0005503 ! two-dimensional polyacrylamide gel electrophoresis evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T11:03:08Z [Term] id: ECO:0005615 name: alkaline phosphatase reporter gene assay evidence used in manual assertion def: "A type of alkaline phosphatase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001801 ! alkaline phosphatase reporter gene assay evidence intersection_of: ECO:0001801 ! alkaline phosphatase reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T11:05:59Z [Term] id: ECO:0005616 name: beta-galactosidase reporter gene assay evidence used in manual assertion def: "A type of beta-galactosidase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001802 ! beta-galactosidase reporter gene assay evidence intersection_of: ECO:0001802 ! beta-galactosidase reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T11:08:57Z [Term] id: ECO:0005617 name: chloramphenicol acetyltransferase reporter gene assay evidence used in manual assertion def: "A type of chloramphenicol acetyltransferase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001803 ! chloramphenicol acetyltransferase reporter gene assay evidence intersection_of: ECO:0001803 ! chloramphenicol acetyltransferase reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T11:11:07Z [Term] id: ECO:0005618 name: chromatin immunoprecipitation-chip evidence used in automatic assertion def: "A type of chromatin immunoprecipitation-chip evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000230 ! chromatin immunoprecipitation-chip evidence is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion intersection_of: ECO:0000230 ! chromatin immunoprecipitation-chip evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-06T11:17:04Z [Term] id: ECO:0005619 name: chromatin immunoprecipitation- exonuclease evidence used in automatic assertion def: "A type of chromatin immunoprecipitation- exonuclease evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion is_a: ECO:0001806 ! chromatin immunoprecipitation- exonuclease evidence intersection_of: ECO:0001806 ! chromatin immunoprecipitation- exonuclease evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-06T11:22:25Z [Term] id: ECO:0005620 name: chromatin immunoprecipitation-PCR evidence used in manual assertion def: "A type of chromatin immunoprecipitation-PCR evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000227 ! chromatin immunoprecipitation-PCR evidence is_a: ECO:0005644 {is_inferred="true"} ! immunoprecipitation evidence used in manual assertion intersection_of: ECO:0000227 ! chromatin immunoprecipitation-PCR evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T11:25:01Z [Term] id: ECO:0005621 name: chromatin immunoprecipitation-seq evidence used in automatic assertion def: "A type of chromatin immunoprecipitation-seq evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000229 ! chromatin immunoprecipitation-seq evidence is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion intersection_of: ECO:0000229 ! chromatin immunoprecipitation-seq evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-06T11:29:13Z [Term] id: ECO:0005622 name: comparative genomics motif search evidence used in manual assertion def: "A type of comparative genomics motif search evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion is_a: ECO:0005534 ! comparative genomics motif search evidence intersection_of: ECO:0005534 ! comparative genomics motif search evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T14:49:36Z [Term] id: ECO:0005623 name: comparative genomics motif search evidence used in automatic assertion def: "A type of comparative genomics motif search evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion is_a: ECO:0005534 ! comparative genomics motif search evidence intersection_of: ECO:0005534 ! comparative genomics motif search evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-06T14:52:21Z [Term] id: ECO:0005624 name: consensus search evidence used in manual assertion def: "A type of consensus search evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion is_a: ECO:0005532 ! consensus search evidence intersection_of: ECO:0005532 ! consensus search evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T14:54:53Z [Term] id: ECO:0005625 name: consensus search evidence used in automatic assertion def: "A type of consensus search evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion is_a: ECO:0005532 ! consensus search evidence intersection_of: ECO:0005532 ! consensus search evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-06T14:58:22Z [Term] id: ECO:0005626 name: copper-phenanthroline footprinting evidence used in manual assertion def: "A type of copper-phenanthroline footprinting evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001815 ! copper-phenanthroline footprinting evidence intersection_of: ECO:0001815 ! copper-phenanthroline footprinting evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T15:01:25Z [Term] id: ECO:0005627 name: DNA adenine methyltransferase identification evidence used in manual assertion def: "A type of DNA adenine methyltransferase identification evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001824 ! DNA adenine methyltransferase identification evidence intersection_of: ECO:0001824 ! DNA adenine methyltransferase identification evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T15:05:14Z [Term] id: ECO:0005628 name: DNA adenine methyltransferase identification evidence used in automatic assertion def: "A type of DNA adenine methyltransferase identification evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion is_a: ECO:0001824 ! DNA adenine methyltransferase identification evidence intersection_of: ECO:0001824 ! DNA adenine methyltransferase identification evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-06T15:06:46Z [Term] id: ECO:0005629 name: DNA affinity chromatography evidence used in manual assertion def: "A type of DNA affinity chromatography evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001809 ! DNA affinity chromatography evidence intersection_of: ECO:0001809 ! DNA affinity chromatography evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T15:08:40Z [Term] id: ECO:0005630 name: DNA microarray evidence used in automatic assertion def: "A type of DNA microarray evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion is_a: ECO:0005524 ! DNA microarray evidence intersection_of: ECO:0005524 ! DNA microarray evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-06T15:31:51Z [Term] id: ECO:0005631 name: DNAse footprinting evidence used in manual assertion def: "A type of DNAse footprinting evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001810 ! DNAse footprinting evidence intersection_of: ECO:0001810 ! DNAse footprinting evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T15:35:41Z [Term] id: ECO:0005632 name: fluorescence anisotropy evidence used in manual assertion def: "A type of fluorescence anisotropy evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0001811 ! fluorescence anisotropy evidence intersection_of: ECO:0001811 ! fluorescence anisotropy evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T15:45:40Z [Term] id: ECO:0005633 name: ferric uptake regulator titration assay evidence used in manual assertion def: "A type of ferric uptake regulator titration assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001829 ! ferric uptake regulator titration assay evidence intersection_of: ECO:0001829 ! ferric uptake regulator titration assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T15:49:18Z [Term] id: ECO:0005634 name: genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion def: "A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001812 ! genomic systematic evolution of ligands by exponential amplification evidence intersection_of: ECO:0001812 ! genomic systematic evolution of ligands by exponential amplification evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T16:41:30Z [Term] id: ECO:0005635 name: genomic systematic evolution of ligands by exponential amplification evidence used in automatic assertion def: "A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion is_a: ECO:0001812 ! genomic systematic evolution of ligands by exponential amplification evidence intersection_of: ECO:0001812 ! genomic systematic evolution of ligands by exponential amplification evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-06T16:43:03Z [Term] id: ECO:0005636 name: green fluorescent protein reporter gene assay evidence used in manual assertion def: "A type of green fluorescent protein reporter gene assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001816 ! green fluorescent protein reporter gene assay evidence intersection_of: ECO:0001816 ! green fluorescent protein reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T16:46:32Z [Term] id: ECO:0005637 name: green fluorescent protein reporter gene assay evidence used in automatic assertion def: "A type of green fluorescent protein reporter gene assay evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion is_a: ECO:0001816 ! green fluorescent protein reporter gene assay evidence intersection_of: ECO:0001816 ! green fluorescent protein reporter gene assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-06T16:48:29Z [Term] id: ECO:0005638 name: cell growth assay evidence used in manual assertion def: "A type of cell growth assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000095 ! cell growth assay evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000095 ! cell growth assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T16:52:15Z [Term] id: ECO:0005639 name: cell growth assay evidence used in automatic assertion def: "A type of cell growth assay evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000095 ! cell growth assay evidence is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion intersection_of: ECO:0000095 ! cell growth assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-06T16:53:28Z [Term] id: ECO:0005640 name: glutathione S-transferase pull-down assay evidence used in manual assertion def: "A type of glutathione S-transferase pull-down assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001817 ! glutathione S-transferase pull-down assay evidence intersection_of: ECO:0001817 ! glutathione S-transferase pull-down assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T16:56:39Z [Term] id: ECO:0005641 name: beta-glucuronidase reporter gene assay evidence used in manual assertion def: "A type of beta-glucuronidase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001804 ! beta-glucuronidase reporter gene assay evidence intersection_of: ECO:0001804 ! beta-glucuronidase reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T16:58:29Z [Term] id: ECO:0005642 name: heteronuclear single quantum coherence spectroscopy evidence used in manual assertion def: "A type of heteronuclear single quantum coherence spectroscopy evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001238 {is_inferred="true"} ! nuclear magnetic resonance spectroscopy evidence used in manual assertion is_a: ECO:0001813 ! heteronuclear single quantum coherence spectroscopy evidence intersection_of: ECO:0001813 ! heteronuclear single quantum coherence spectroscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T23:10:13Z [Term] id: ECO:0005643 name: hydroxyl-radical footprinting evidence used in manual assertion def: "A type of hydroxyl-radical footprinting evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001818 ! hydroxyl-radical footprinting evidence intersection_of: ECO:0001818 ! hydroxyl-radical footprinting evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T23:14:48Z [Term] id: ECO:0005644 name: immunoprecipitation evidence used in manual assertion def: "A type of immunoprecipitation evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000085 ! immunoprecipitation evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000085 ! immunoprecipitation evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T23:20:49Z [Term] id: ECO:0005645 name: interferometric reflectance imaging sensor evidence used in manual assertion def: "A type of interferometric reflectance imaging sensor evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0005520 ! interferometric reflectance imaging sensor evidence intersection_of: ECO:0005520 ! interferometric reflectance imaging sensor evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T23:27:58Z [Term] id: ECO:0005646 name: interferometric reflectance imaging sensor evidence used in automatic assertion def: "A type of interferometric reflectance imaging sensor evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion is_a: ECO:0005520 ! interferometric reflectance imaging sensor evidence intersection_of: ECO:0005520 ! interferometric reflectance imaging sensor evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-06T23:29:23Z [Term] id: ECO:0005647 name: isothermal titration calorimetry evidence used in manual assertion def: "A type of isothermal titration calorimetry evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001825 ! isothermal titration calorimetry evidence intersection_of: ECO:0001825 ! isothermal titration calorimetry evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T23:36:26Z [Term] id: ECO:0005648 name: luciferase reporter gene assay evidence used in manual assertion def: "A type of luciferase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001805 ! luciferase reporter gene assay evidence intersection_of: ECO:0001805 ! luciferase reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T23:44:52Z [Term] id: ECO:0005649 name: machine learning prediction of motif instance evidence used in manual assertion def: "A type of machine learning prediction of motif instance evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000255 {is_inferred="true"} ! match to sequence model evidence used in manual assertion is_a: ECO:0005535 ! machine learning prediction of motif instance evidence intersection_of: ECO:0005535 ! machine learning prediction of motif instance evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-06T23:51:30Z [Term] id: ECO:0005650 name: machine learning prediction of motif instance evidence used in automatic assertion def: "A type of machine learning prediction of motif instance evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000256 {is_inferred="true"} ! match to sequence model evidence used in automatic assertion is_a: ECO:0005535 ! machine learning prediction of motif instance evidence intersection_of: ECO:0005535 ! machine learning prediction of motif instance evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-06T23:54:00Z [Term] id: ECO:0005651 name: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in automatic assertion def: "A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion is_a: ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence intersection_of: ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-06T23:57:30Z [Term] id: ECO:0005652 name: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in manual assertion def: "A type of methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001814 ! methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence intersection_of: ECO:0001814 ! methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-09T14:42:28Z [Term] id: ECO:0005653 name: northern assay evidence used in manual assertion def: "A type of northern assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000106 ! northern assay evidence is_a: ECO:0000286 {is_inferred="true"} ! transcript expression level evidence used in manual assertion intersection_of: ECO:0000106 ! northern assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-09T14:48:03Z [Term] id: ECO:0005654 name: phylogenetic footprinting evidence used in manual assertion def: "A type of phylogenetic footprinting evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0005533 ! phylogenetic footprinting evidence is_a: ECO:0005558 {is_inferred="true"} ! motif discovery evidence used in manual assertion intersection_of: ECO:0005533 ! phylogenetic footprinting evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-09T14:50:52Z [Term] id: ECO:0005655 name: phylogenetic footprinting evidence used in automatic assertion def: "A type of phylogenetic footprinting evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0005533 ! phylogenetic footprinting evidence is_a: ECO:0005559 {is_inferred="true"} ! motif discovery evidence used in automatic assertion intersection_of: ECO:0005533 ! phylogenetic footprinting evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-09T14:51:55Z [Term] id: ECO:0005656 name: premethylation interference footprinting evidence used in manual assertion def: "A type of premethylation interference footprinting evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001827 ! premethylation interference footprinting evidence intersection_of: ECO:0001827 ! premethylation interference footprinting evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-09T14:55:25Z [Term] id: ECO:0005657 name: primer extension assay evidence used in manual assertion def: "A type of primer extension assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000286 {is_inferred="true"} ! transcript expression level evidence used in manual assertion is_a: ECO:0001819 ! primer extension assay evidence intersection_of: ECO:0001819 ! primer extension assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-09T14:58:38Z [Term] id: ECO:0005658 name: position-specific scoring matrix motif search evidence used in manual assertion def: "A type of position-specific scoring matrix motif search evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion is_a: ECO:0005536 ! position-specific scoring matrix motif search evidence intersection_of: ECO:0005536 ! position-specific scoring matrix motif search evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-09T15:01:31Z [Term] id: ECO:0005659 name: position-specific scoring matrix motif search evidence used in automatic assertion def: "A type of position-specific scoring matrix motif search evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion is_a: ECO:0005536 ! position-specific scoring matrix motif search evidence intersection_of: ECO:0005536 ! position-specific scoring matrix motif search evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-09T15:02:31Z [Term] id: ECO:0005660 name: quantitative polymerase chain reaction evidence used in manual assertion def: "A type of quantitative polymerase chain reaction evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000231 ! quantitative polymerase chain reaction evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000231 ! quantitative polymerase chain reaction evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-09T15:09:01Z [Term] id: ECO:0005661 name: rapid amplification of cDNA ends polymerase chain reaction evidence used in manual assertion def: "A type of rapid amplification of cDNA ends polymerase chain reaction evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001820 ! rapid amplification of cDNA ends polymerase chain reaction evidence intersection_of: ECO:0001820 ! rapid amplification of cDNA ends polymerase chain reaction evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-09T15:19:20Z [Term] id: ECO:0005662 name: regular expression motif search evidence used in manual assertion def: "A type of regular expression motif search evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion is_a: ECO:0005505 ! regular expression motif search evidence intersection_of: ECO:0005505 ! regular expression motif search evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-09T16:13:55Z [Term] id: ECO:0005663 name: regular expression motif search evidence used in automatic assertion def: "A type of regular expression motif search evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion is_a: ECO:0005505 ! regular expression motif search evidence intersection_of: ECO:0005505 ! regular expression motif search evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-09T16:15:03Z [Term] id: ECO:0005664 name: RNA sequencing assay evidence used in manual assertion def: "A type of RNA sequencing assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001821 ! RNA sequencing assay evidence intersection_of: ECO:0001821 ! RNA sequencing assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-09T16:18:35Z [Term] id: ECO:0005665 name: RNA sequencing assay evidence used in automatic assertion def: "A type of RNA sequencing assay evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion is_a: ECO:0001821 ! RNA sequencing assay evidence intersection_of: ECO:0001821 ! RNA sequencing assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-09T16:19:38Z [Term] id: ECO:0005666 name: S1 nuclease protection assay evidence used in manual assertion def: "A type of S1 nuclease protection assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001240 {is_inferred="true"} ! nuclease protection assay evidence used in manual assertion is_a: ECO:0005521 ! S1 nuclease protection assay evidence intersection_of: ECO:0005521 ! S1 nuclease protection assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-09T16:22:58Z [Term] id: ECO:0005667 name: site-directed mutagenesis evidence used in manual assertion def: "A type of site-directed mutagenesis evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0005528 ! site-directed mutagenesis evidence intersection_of: ECO:0005528 ! site-directed mutagenesis evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-09T16:29:13Z [Term] id: ECO:0005668 name: survival rate analysis evidence used in manual assertion def: "A type of survival rate analysis evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001225 {is_inferred="true"} ! knockout evidence used in manual assertion is_a: ECO:0001822 ! survival rate analysis evidence intersection_of: ECO:0001822 ! survival rate analysis evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-09T16:31:08Z [Term] id: ECO:0005669 name: ultraviolet light footprinting evidence used in manual assertion def: "A type of ultraviolet light footprinting evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001826 ! ultraviolet light footprinting evidence intersection_of: ECO:0001826 ! ultraviolet light footprinting evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-09T16:33:44Z [Term] id: ECO:0005670 name: x-ray crystallography evidence used in manual assertion def: "A type of x-ray crystallography evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001171 {is_inferred="true"} ! crystallography evidence used in manual assertion is_a: ECO:0001823 ! x-ray crystallography evidence intersection_of: ECO:0001823 ! x-ray crystallography evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-10T11:53:00Z [Term] id: ECO:0005671 name: x-ray crystallography evidence used in automatic assertion def: "A type of x-ray crystallography evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion is_a: ECO:0001823 ! x-ray crystallography evidence intersection_of: ECO:0001823 ! x-ray crystallography evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion created_by: snadendla creation_date: 2016-05-10T11:54:03Z [Term] id: ECO:0005672 name: xylE reporter gene assay evidence used in manual assertion def: "A type of xylE reporter gene assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0005537 ! xylE reporter gene assay evidence intersection_of: ECO:0005537 ! xylE reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-10T11:57:15Z [Term] id: ECO:0005673 name: ad-hoc qualitative phenotype observation evidence def: "A type of experimental phenotypic evidence where a non-standard trait (e.g. natural competence) is assessed qualitatively (e.g. presence/absence) and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor." [ECO:SN, PMID:15150239] is_a: ECO:0000059 ! experimental phenotypic evidence created_by: snadendla creation_date: 2016-05-10T12:57:41Z [Term] id: ECO:0005674 name: ad-hoc qualitative phenotype observation evidence used in manual assertion def: "A type of ad-hoc qualitative phenotype observation that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0005673 ! ad-hoc qualitative phenotype observation evidence intersection_of: ECO:0005673 ! ad-hoc qualitative phenotype observation evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-10T14:28:47Z [Term] id: ECO:0005675 name: ad-hoc quantitative phenotype observation evidence def: "A type of experimental phenotypic evidence where a quantifiable phenotypic trait (e.g. glucose intake) is assessed in a quantitative manner after induction of the regulatory mechanism and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor." [ECO:SN, PMID:15150239] is_a: ECO:0000059 ! experimental phenotypic evidence created_by: snadendla creation_date: 2016-05-10T14:30:50Z [Term] id: ECO:0005676 name: ad-hoc quantitative phenotype observation evidence used in manual assertion def: "A type of ad-hoc quantitative phenotype observation evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0005675 ! ad-hoc quantitative phenotype observation evidence intersection_of: ECO:0005675 ! ad-hoc quantitative phenotype observation evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: snadendla creation_date: 2016-05-10T14:34:50Z [Term] id: ECO:0005800 name: dilution assay evidence def: "A direct assay evidence where the activity of a solution is determined by exposing an indicator culture or organism to a series of dilutions and determining the lowest concentration that elicits a biological response." [http://www.sciencedirect.com/science/article/pii/016770129400068I] comment: For chemicals, bacteriocins, or phage, the indicator culture is typically present in a soft-agar layer on top of nutrient agar and a small quantity of each dilution is spotted onto the soft agar overlay. synonym: "critical dilution assay" EXACT [] synonym: "spot assay" EXACT [] is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0005801 name: enzyme assay evidence used in manual assertion def: "A type of enzyme assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000005 ! enzyme assay evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000005 ! enzyme assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 ! manual assertion [Term] id: ECO:0005802 name: cell transfection experiment evidence used in manual assertion def: "A type of cell transfection experiment evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0005606 ! cell transfection experiment evidence intersection_of: ECO:0005606 ! cell transfection experiment evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 ! manual assertion [Term] id: ECO:0005803 name: motility assay evidence def: "A type of direct assay evidence where the motility of a cell or cells is determined." [ECO:SN] is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0005804 name: immunofluorescence evidence used in manual assertion def: "A type of immunofluorescence evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000007 ! immunofluorescence evidence is_a: ECO:0000269 ! experimental evidence used in manual assertion intersection_of: ECO:0000007 ! immunofluorescence evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 ! manual assertion [Term] id: ECO:0005805 name: yeast 2-hybrid evidence used in manual assertion def: "A type of yeast 2-hybrid evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000068 ! yeast 2-hybrid evidence is_a: ECO:0000353 ! physical interaction evidence used in manual assertion intersection_of: ECO:0000068 ! yeast 2-hybrid evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 ! manual assertion [Term] id: ECO:0006001 name: Cya fusion reporter assay evidence def: "A type of evidence derived from exploiting the CyaA (calmodulin-activated adenylate cyclase toxin) secreted by Bordatella pertussis to synthesize cAMP and alter cellular physiology in a host cell for various biological applications." [PMID:10217833, PMID:14702323] is_a: ECO:0000017 ! ectopic expression evidence is_a: ECO:0000049 ! reporter gene assay evidence created_by: rctauber creation_date: 2016-06-15T08:42:16Z [Term] id: ECO:0006002 name: Cya fusion reporter assay evidence used in manual assertion def: "A type of Cya fusion reporter assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001273 {is_inferred="true"} ! ectopic expression evidence used in manual assertion is_a: ECO:0006001 ! Cya fusion reporter assay evidence intersection_of: ECO:0006001 ! Cya fusion reporter assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2010-06-15T08:44:10Z [Term] id: ECO:0006003 name: electron microscopy evidence used in manual assertion def: "A type of electron microscopy evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0005033 ! electron microscopy evidence intersection_of: ECO:0005033 ! electron microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2016-10-12T12:32:29Z [Term] id: ECO:0006004 name: super-resolution microscopy evidence used in manual assertion def: "A type of super-resolution microscopy evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0005578 ! super-resolution microscopy evidence intersection_of: ECO:0005578 ! super-resolution microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2016-10-12T12:32:29Z [Term] id: ECO:0006005 name: fractionation evidence used in manual assertion def: "A type of fractionation evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000100 ! fractionation evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000100 ! fractionation evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2016-10-12T12:32:29Z [Term] id: ECO:0006006 name: electrophysiology assay evidence used in manual assertion def: "A type of electrophysiology assay evidence that is used in a manual assertion." [] {comment="ECO:RCT"} is_a: ECO:0000164 ! electrophysiology assay evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000164 ! electrophysiology assay evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2016-10-12T12:32:29Z [Term] id: ECO:0006007 name: chromatin immunoprecipitation-chip evidence used in manual assertion def: "A type of chromatin immunoprecipitation-chip evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000230 ! chromatin immunoprecipitation-chip evidence is_a: ECO:0005644 ! immunoprecipitation evidence used in manual assertion intersection_of: ECO:0000230 ! chromatin immunoprecipitation-chip evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2016-10-21T11:55:53Z [Term] id: ECO:0006008 name: chromatin immunoprecipitation- exonuclease evidence used in manual assertion def: "A type of chromatin immunoprecipitation- exonuclease evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001806 ! chromatin immunoprecipitation- exonuclease evidence is_a: ECO:0005644 ! immunoprecipitation evidence used in manual assertion intersection_of: ECO:0001806 ! chromatin immunoprecipitation- exonuclease evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2016-10-21T11:55:53Z [Term] id: ECO:0006009 name: chromatin immunoprecipitation-seq evidence used in manual assertion def: "A type of chromatin immunoprecipitation-seq evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000229 ! chromatin immunoprecipitation-seq evidence is_a: ECO:0005644 ! immunoprecipitation evidence used in manual assertion intersection_of: ECO:0000229 ! chromatin immunoprecipitation-seq evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2016-10-21T11:55:53Z [Term] id: ECO:0006010 name: mRNA interactome capture evidence def: "A type of nucleic acid binding evidence where RNA and proteins are cross-linked by ultraviolet light and RNA-protein complexes are captured on oligo(dT) beads. After RNAse treatment, the RNA-bound proteins are identified by mass spectrometry." [PMID:27729395] synonym: "ultraviolet light cross-linking RNA binding evidence" EXACT [] is_a: ECO:0000136 ! nucleic acid binding evidence created_by: rctauber creation_date: 2016-10-21T12:28:17Z [Term] id: ECO:0006011 name: mRNA interactome capture evidence used in manual assertion def: "A type of mRNA interactome capture evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000353 ! physical interaction evidence used in manual assertion is_a: ECO:0006010 ! mRNA interactome capture evidence intersection_of: ECO:0006010 ! mRNA interactome capture evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2016-10-21T12:28:17Z [Typedef] id: used_in name: used_in def: "A relation connecting a piece of evidence to an assertion method, where that assertion method is supported by the evidence." [GOC:cjm] comment: In the future we may use a more generic relation with weaker domain and range constraints taken from IAO, RO or OBI. xref: ECO:9000000 domain: ECO:0000000 ! evidence range: ECO:0000217 ! assertion method inverse_of: uses ! uses created_by: mchibucos creation_date: 2010-12-09T05:00:20Z [Typedef] id: uses name: uses xref: ECO:9000001