Class ID,Preferred Label,Synonyms,Definitions,Obsolete,CUI,Semantic Types,Parents,contains_class,example_of,has_example,has_technique,http://www.w3.org/2004/02/skos/core#altLabel,http://www.w3.org/2004/02/skos/core#definition,http://www.w3.org/2004/02/skos/core#example,http://www.w3.org/2004/02/skos/core#note,resource,see_also,subclass_of,technique_used_in
http://webprotege.stanford.edu/RNnnUUWkOOSotXzfVJfzY5,megaTAL target site,,"intended DNA binding site of a megaTAL, encompassing the DNA sequence targeted by both the TAL array and the meganuclease",false,,,http://webprotege.stanford.edu/RBY90WCUDrMkchP0gyrGtbh,,,,,,"intended DNA binding site of a megaTAL, encompassing the DNA sequence targeted by both the TAL array and the meganuclease",,,,,,
http://webprotege.stanford.edu/R73yDdBWgvBpa27Obs1Kx8v,RNP,ribonucleoprotein,complex comprising protein bound to RNA,false,,,http://webprotege.stanford.edu/RFC8IXWXpXXKENPLyDDQ3x,,,,,ribonucleoprotein,complex comprising protein bound to RNA,,"In the context of CRISPR-based genome editing, RNP refers to the complex of Cas protein(s) and gRNA",,,,
http://webprotege.stanford.edu/R8OS4Hf3z33O6kQfiFLGBTZ,meganuclease target site,,DNA sequence recognized by meganucleases,false,,,http://webprotege.stanford.edu/RBGB11OaBgqnhWBlhSXHkmN,,,,,,DNA sequence recognized by meganucleases,,Meganuclease target sites are 15 to 40 base pair DNA sequence consisting of two equal length half sites separated by a 4 base pair middle sequence (also known as “central 4”). Cleavage occurs at the junction of the half sites and the middle site on each DNA strand leaving a 4 nucleotide 3′ overhang.,,,,
http://webprotege.stanford.edu/RCdyrwkluvMWRM0pURVcLuZ,sgRNA,single-guide RNA,fusion of crRNA and tracrRNA,false,,,http://webprotege.stanford.edu/RFC8IXWXpXXKENPLyDDQ3x,,,,,single-guide RNA,fusion of crRNA and tracrRNA,,,,,,
http://webprotege.stanford.edu/RDKoziwo8iqNAptnBqa6Sfu,specificity,genome editing target specificity,extent to which an editing agent or procedure acts only on its intended target,false,,,http://webprotege.stanford.edu/RBS6TjeRZpEijUCrPDECjgs,,,,,genome editing target specificity,extent to which an editing agent or procedure acts only on its intended target,,"When using this term, the procedure is defined, the intended target is defined, the action or outcome is measured and reported, and limits of detection are reported.",,,,
http://webprotege.stanford.edu/R9n4XS1nbDSxSPXtyUKaRbz,Cas nuclease,CRISPR associated nuclease,enzyme that is a component of CRISPR systems that is capable of breaking the phosphodiester bonds between nucleotides,false,,,http://webprotege.stanford.edu/RFC8IXWXpXXKENPLyDDQ3x,,,,,CRISPR associated nuclease,enzyme that is a component of CRISPR systems that is capable of breaking the phosphodiester bonds between nucleotides,"Cas3, Cas9, Cas12a, Cas13, CasX.",Some but not all Cas nucleases interact with a gRNA.,,,,
http://webprotege.stanford.edu/RFC8IXWXpXXKENPLyDDQ3x,CRISPR specific,,,false,,,http://webprotege.stanford.edu/RDyioNYXAgMHUox9J3W3veJ,,,,,,,,,,,,
http://webprotege.stanford.edu/R8MVdbpXg5I7FXNvV1aOHWs,zinc finger,Cys2His2 zinc finger,DNA binding domain that folds via coordination of zinc into a compact structure consisting of two beta strands and one alpha-helix (β β α),false,,,http://webprotege.stanford.edu/R7XppaHUTo1MnKzZFHCqU6I,,,,,Cys2His2 zinc finger,DNA binding domain that folds via coordination of zinc into a compact structure consisting of two beta strands and one alpha-helix (β β α),,Zinc finger DNA binding domains typically contain 28 amino acids.,,,,
http://webprotege.stanford.edu/RJTVqbdMfdsLmbysCUpcTF,genome engineering,,process of introducing intentional changes to genomic nucleic acid,false,,,http://webprotege.stanford.edu/RBS6TjeRZpEijUCrPDECjgs,,,,,,process of introducing intentional changes to genomic nucleic acid,,,,,,
http://webprotege.stanford.edu/R9Y7eOqJCend6Bs94rQFz0S,TALEN specific,,,false,,,http://webprotege.stanford.edu/RDyioNYXAgMHUox9J3W3veJ,,,,,,,,,,,,
http://webprotege.stanford.edu/RBY90WCUDrMkchP0gyrGtbh,megaTAL specific,,,false,,,http://webprotege.stanford.edu/RDyioNYXAgMHUox9J3W3veJ,,,,,,,,,,,,
http://webprotege.stanford.edu/R1PtcA95daempqxgAhccoQ,TALEN target site,,DNA sequence recognized by TALENs,false,,,http://webprotege.stanford.edu/R9Y7eOqJCend6Bs94rQFz0S,,,,,,DNA sequence recognized by TALENs,,Typical TALEN target sites are recognized by a pair of TALENs and contain a central spacer region flanked by upstream and downstream sequences that are each recognized by one TALEN. This pair is designed in such a way that two TALEN nuclease domains dimerize to cleave DNA within the spacer region.,,,,
http://webprotege.stanford.edu/RCYSyxkNxG5scKoB5aiwAck,repair template,,nucleic acid sequence used to direct cellular DNA repair pathways to incorporate specific DNA sequence changes at or near a target,false,,,http://webprotege.stanford.edu/RDyioNYXAgMHUox9J3W3veJ,,,,,,nucleic acid sequence used to direct cellular DNA repair pathways to incorporate specific DNA sequence changes at or near a target,,,,,,
http://webprotege.stanford.edu/RBzHwRfenFywlsvIWKCGOwA,tracrRNA,trans-activating CRISPR RNA,polyribonucleotide that base-pairs with the crRNA and interacts with a Cas nuclease to enable sequence-specific interaction of the target,false,,,http://webprotege.stanford.edu/RFC8IXWXpXXKENPLyDDQ3x,,,,,trans-activating CRISPR RNA,polyribonucleotide that base-pairs with the crRNA and interacts with a Cas nuclease to enable sequence-specific interaction of the target,,tracrRNA is an optional component of gRNA,,,,
http://webprotege.stanford.edu/RJzgyRf50JMDJXIJ6RtJIq,megaTAL,,"artificial chimeric nucleases composed of an array of transcription activator-like (TAL) effector (TALE) DNA binding domains, a megaTAL linker and a meganuclease",false,,,http://webprotege.stanford.edu/RBY90WCUDrMkchP0gyrGtbh,,,,,,"artificial chimeric nucleases composed of an array of transcription activator-like (TAL) effector (TALE) DNA binding domains, a megaTAL linker and a meganuclease",,,,,,
http://webprotege.stanford.edu/RBeSPrSMVdrNabIa4CoV9uQ,intended edit,,designed modification to a target resulting from the application of genome editing components,false,,,http://webprotege.stanford.edu/RjAHARFJxxBxFl0vcXU0HQ,,,,,,designed modification to a target resulting from the application of genome editing components,,Genome editing components can include a nuclease and repair template,,,,
http://webprotege.stanford.edu/R7bjSuHikDpW1ZicN2xO2hy,site-directed DNA modification enzyme,,enzyme capable of modifying DNA at a specific sequence,false,,,http://webprotege.stanford.edu/RDyioNYXAgMHUox9J3W3veJ,,,,,,enzyme capable of modifying DNA at a specific sequence,,,,,,
http://webprotege.stanford.edu/RBNTuBSmC3bhzS0S7XjiK6n,TALEN linker,,"polypeptide sequence that links an array of TAL DNA binding domains and an endodeoxyribonuclease, typically FokI",false,,,http://webprotege.stanford.edu/R9Y7eOqJCend6Bs94rQFz0S,,,,,,"polypeptide sequence that links an array of TAL DNA binding domains and an endodeoxyribonuclease, typically FokI",,,,,,
http://webprotege.stanford.edu/RBS6TjeRZpEijUCrPDECjgs,Genome editing concepts,,,false,,,http://webprotege.stanford.edu/RrxffP8RsuY7DyOnYLOkIu,,,,,,,,,,,,
http://webprotege.stanford.edu/RIX03OgrDh0KO9wdHKleoH,site-directed nuclease,sequence-specific nuclease,enzyme capable of cleaving the phosphodiester bond between adjacent nucleotides in a nucleic acid polymer at a specific sequence,false,,,http://webprotege.stanford.edu/RDyioNYXAgMHUox9J3W3veJ,,,,,sequence-specific nuclease,enzyme capable of cleaving the phosphodiester bond between adjacent nucleotides in a nucleic acid polymer at a specific sequence,,,,,,
http://webprotege.stanford.edu/RxZG0bQrlsmry28PNc3xov,gRNA,guide RNA,polyribonucleotide containing regions sufficient for productive interaction with a Cas nuclease (3.2.2.1) or variant to direct interaction with the specific target,false,,,http://webprotege.stanford.edu/RFC8IXWXpXXKENPLyDDQ3x,,,,,guide RNA,polyribonucleotide containing regions sufficient for productive interaction with a Cas nuclease (3.2.2.1) or variant to direct interaction with the specific target,,"sgRNA for Cas9 proteins are non-naturally occurring polyribonucleotides where the crRNA and tracrRNA are fused with an artificial linker.|In some cases, chemical modifications of the polyribonucleotide are used, such as modifications to the phosphodiester linkages, bases or sugar moieties. These can include substitution of DNA (2′-deoxy) or 2′-methoxy nucleotides for RNA nucleotides, etc.|For Cas9-type proteins, the natural gRNA comprises a crRNA that imparts sequence specificity and the tracrRNA that interacts with and activates the protein. This is sometimes referred to as a “dual guide”. Other Cas proteins can have different gRNA structures.",,,,
http://webprotege.stanford.edu/RtcB1WPsw1y2PbJNpBrfWB,megaTAL linker,,amino acid sequence that links an array of TAL DNA binding domains and a meganuclease,false,,,http://webprotege.stanford.edu/RBY90WCUDrMkchP0gyrGtbh,,,,,,amino acid sequence that links an array of TAL DNA binding domains and a meganuclease,,,,,,
http://webprotege.stanford.edu/RBGB11OaBgqnhWBlhSXHkmN,Meganuclease specific,,,false,,,http://webprotege.stanford.edu/RDyioNYXAgMHUox9J3W3veJ,,,,,,,,,,,,
http://webprotege.stanford.edu/RkZZ2jxUXPST3XdcEwZI51,target,genome editing target,"nucleic acid sequence subject to intentional binding, modification and/or cleavage during a genome editing process",false,,,http://webprotege.stanford.edu/RBS6TjeRZpEijUCrPDECjgs,,,,,genome editing target,"nucleic acid sequence subject to intentional binding, modification and/or cleavage during a genome editing process",,,,,,
http://webprotege.stanford.edu/R8Yl1w1QG1g2QIvJ83Oc2q8,meganuclease linker,,natural or artificially derived polypeptide sequence that links two LAGLIDADG domains to one another to form a single polypeptide chain,false,,,http://webprotege.stanford.edu/RBGB11OaBgqnhWBlhSXHkmN,,,,,,natural or artificially derived polypeptide sequence that links two LAGLIDADG domains to one another to form a single polypeptide chain,,The LAGLIDADG consensus sequence represents an alpha helix that serves as a dimerization interface and key component in the DNA cleavage site in this family of meganucleases,,,,
http://webprotege.stanford.edu/R7mzHLx97HO2yOJcnj9BK3g,Genome editing tools,,,false,,,http://webprotege.stanford.edu/RrxffP8RsuY7DyOnYLOkIu,,,,,,,,,,,,
http://webprotege.stanford.edu/RC2MgsCJHlZLNI5AOCVuKBH,gene editing,,"techniques for genome engineering that involve nucleic acid damage, repair mechanisms, replication and/or recombination for incorporating site-specific modification(s) into a gene or genes",false,,,http://webprotege.stanford.edu/RBS6TjeRZpEijUCrPDECjgs,,,,,,"techniques for genome engineering that involve nucleic acid damage, repair mechanisms, replication and/or recombination for incorporating site-specific modification(s) into a gene or genes",,There are various genome editing tools,,,,
http://webprotege.stanford.edu/R90tuRKc3hyftbruhRem3cc,off-target,genome editing off-target,genomic position and/or nucleic acid sequence distinct from the target,false,,,http://webprotege.stanford.edu/RBS6TjeRZpEijUCrPDECjgs,,,,,genome editing off-target,genomic position and/or nucleic acid sequence distinct from the target,"Off-target binding, off-target cleavage, off-target edit, off-target sequence change.",,,,,
http://webprotege.stanford.edu/RrxffP8RsuY7DyOnYLOkIu,Biotechnology — Genome editing — Part 1: Vocabulary,,,false,,,http://www.w3.org/2002/07/owl#Thing,,,,,,,,,,,,
http://webprotege.stanford.edu/RBqUPv4VNR9dIptjsv5o7ym,ZFN target site,,DNA sequence recognized by a pair of ZFNs,false,,,http://webprotege.stanford.edu/R7XppaHUTo1MnKzZFHCqU6I,,,,,,DNA sequence recognized by a pair of ZFNs,,Typical ZFN target sites contain a central spacer region flanked by DNA sequences that are each recognized by an array of zinc fingers oriented such that the ZFN nuclease domains dimerize and cleave within the spacer.,,,,
http://webprotege.stanford.edu/R91RGzchUqGXUSLSCuXdSQE,genome editing,,"techniques for genome engineering that involve nucleic acid damage, repair mechanisms, replication and/or recombination for incorporating site-specific modification(s) into a genomic DNA",false,,,http://webprotege.stanford.edu/RBS6TjeRZpEijUCrPDECjgs,,,,,,"techniques for genome engineering that involve nucleic acid damage, repair mechanisms, replication and/or recombination for incorporating site-specific modification(s) into a genomic DNA",,There are various genome editing tools,,,,
http://webprotege.stanford.edu/R9OV53gGiuBaFvbin4CLpMu,ZFP,zinc finger protein,DNA binding protein consisting of a tandem array of multiple zinc fingers,false,,,http://webprotege.stanford.edu/R7XppaHUTo1MnKzZFHCqU6I,,,,,zinc finger protein,DNA binding protein consisting of a tandem array of multiple zinc fingers,,,,,,
http://webprotege.stanford.edu/RBtHXpvGEKLDqCAickoBnPj,MMEJ,microhomology-mediated end joining repair,mechanism of DNA end-joining repair where the DNA ends are rejoined to each other using short regions of homology flanking the initiating double-stranded break to align the ends for repair,false,,,http://webprotege.stanford.edu/RjAHARFJxxBxFl0vcXU0HQ,,,,,microhomology-mediated end joining repair,mechanism of DNA end-joining repair where the DNA ends are rejoined to each other using short regions of homology flanking the initiating double-stranded break to align the ends for repair,,MMEJ repair of DNA breaks in genome editing approaches can result in deletion between pairs of microhomology regions.|Short regions of homology for MMEJ are typically 2 to 25 base pairs.,,,,
http://webprotege.stanford.edu/R9YZbcQ45ikaxNbQVzed4zS,PAM,protospacer adjacent motif,short nucleotide motif in the targeted region of nucleic acid required for guided Cas nuclease or variant binding,false,,,http://webprotege.stanford.edu/RFC8IXWXpXXKENPLyDDQ3x,,,,,protospacer adjacent motif,short nucleotide motif in the targeted region of nucleic acid required for guided Cas nuclease or variant binding,,"PAMs are distinct from, but in close proximity to, nucleic acid sequence targeted by gRNA",,,,
http://webprotege.stanford.edu/R7gYuCELexgAHsLmro8BVwy,meganuclease single chain,,meganuclease composed of two LAGLIDADG domains joined by either a natural or artificially derived polypeptide linker in order to be expressed as a single polypeptide chain,false,,,http://webprotege.stanford.edu/RBGB11OaBgqnhWBlhSXHkmN,,,,,,meganuclease composed of two LAGLIDADG domains joined by either a natural or artificially derived polypeptide linker in order to be expressed as a single polypeptide chain,,The LAGLIDADG consensus sequence represents an alpha helix that serves as a dimerization interface and key component in the DNA cleavage site in this family of meganucleases.,,,,
http://webprotege.stanford.edu/R7pLZQYO7i1NE2AfdyH3YfW,crRNA,CRISPR RNA,polyribonucleotide that includes sequence complementarity to the target (3.1.6) and a sequence that interacts with a Cas protein and optionally tracrRNA,false,,,http://webprotege.stanford.edu/RFC8IXWXpXXKENPLyDDQ3x,,,,,CRISPR RNA,polyribonucleotide that includes sequence complementarity to the target (3.1.6) and a sequence that interacts with a Cas protein and optionally tracrRNA,,"In some CRISPR systems, a portion of the crRNA will base-pair with the tracrRNA (e.g. Cas9). Other CRISPR systems lack tracrRNA (e.g. Cas12a/Cpf1). In systems that do not require tracrRNA, the gRNA is called a “CRISPR RNA” or simply “crRNA”.|crRNA is a component of gRNA or a complete gRNA, depending on the CRISPR system.",,,,
http://webprotege.stanford.edu/RjAHARFJxxBxFl0vcXU0HQ,Genome editing outcomes,,,false,,,http://webprotege.stanford.edu/RrxffP8RsuY7DyOnYLOkIu,,,,,,,,,,,,
http://webprotege.stanford.edu/R9PYIewwEMU8AGssmmzZ8cG,meganuclease,,variant of the LAGLIDADG subtype of homing endonucleases engineered to recognize a 15 to 40 base pair DNA target different from the site recognized by the parent endonuclease,false,,,http://webprotege.stanford.edu/RBGB11OaBgqnhWBlhSXHkmN,,,,,,variant of the LAGLIDADG subtype of homing endonucleases engineered to recognize a 15 to 40 base pair DNA target different from the site recognized by the parent endonuclease,,The LAGLIDADG consensus sequence represents an alpha helix that serves as a dimerization interface and key component in the DNA cleavage site in this family of meganucleases.,,,,
http://webprotege.stanford.edu/R8VDQESe797sAKVuDZCJJZ2,TALEN,transcription activator-like effector nuclease,artificial nuclease composed of an endodeoxyribonuclease fused to DNA-binding domains of TALEs that cleave DNA at a defined distance from TALE recognition sequences,false,,,http://webprotege.stanford.edu/R9Y7eOqJCend6Bs94rQFz0S,,,,,transcription activator-like effector nuclease,artificial nuclease composed of an endodeoxyribonuclease fused to DNA-binding domains of TALEs that cleave DNA at a defined distance from TALE recognition sequences,,A TALEN can refer to a pair of TALE-FokI fusion proteins that dimerize on opposite strands of DNA adjacent to a target for cleavage.,,,,
http://webprotege.stanford.edu/RDyioNYXAgMHUox9J3W3veJ,General,,,false,,,http://webprotege.stanford.edu/R7mzHLx97HO2yOJcnj9BK3g,,,,,,,,,,,,
http://webprotege.stanford.edu/R8YZetJ9aNO26XdCGfNXgxJ,indel,InDel mutation,sequence change caused by the insertion and/or deletion of nucleotides,false,,,http://webprotege.stanford.edu/RjAHARFJxxBxFl0vcXU0HQ,,,,,InDel mutation,sequence change caused by the insertion and/or deletion of nucleotides,,,,,,
http://webprotege.stanford.edu/R7seWaMMlKiTKeEn2ygQXkW,RVDs,repeat variable diresidue,two amino acid sequence in TAL repeats that imparts DNA binding specificity,false,,,http://webprotege.stanford.edu/R9Y7eOqJCend6Bs94rQFz0S,,,,,repeat variable diresidue,two amino acid sequence in TAL repeats that imparts DNA binding specificity,,,,,,
http://webprotege.stanford.edu/RCTZ40H12PNPuVn0f1P3pe2,ZFN linker,,polypeptide sequence that links an array of zinc finger binding domains and a DNA cleavage domain,false,,,http://webprotege.stanford.edu/R7XppaHUTo1MnKzZFHCqU6I,,,,,,polypeptide sequence that links an array of zinc finger binding domains and a DNA cleavage domain,,FokI is prevalently used as the DNA cleavage domain bound to a zinc finger.,,,,
http://webprotege.stanford.edu/R5Tb5vt2npuCGMsYcXKGWG,target strand,CRISPR target strand,single-stranded nucleic acid sequence that is complementary to the gRNA of a Cas protein or variant,false,,,http://webprotege.stanford.edu/RFC8IXWXpXXKENPLyDDQ3x,,,,,CRISPR target strand,single-stranded nucleic acid sequence that is complementary to the gRNA of a Cas protein or variant,,,,,,
http://webprotege.stanford.edu/RRKfhPS7OjfRyQTs19h8Pl,Cas nuclease target site,,"nucleotide sequence comprising the PAM, in most cases, and a region that hybridizes to the target sequence specific guide of a Cas RNP",false,,,http://webprotege.stanford.edu/RFC8IXWXpXXKENPLyDDQ3x,,,,,,"nucleotide sequence comprising the PAM, in most cases, and a region that hybridizes to the target sequence specific guide of a Cas RNP",,,,,,
http://webprotege.stanford.edu/Rt68NegpsIxMIoKnwLTsfD,NHEJ,non-homologous end joining,mechanism of DNA end-joining repair in which DNA ends are joined in a homology-independent manner,false,,,http://webprotege.stanford.edu/RjAHARFJxxBxFl0vcXU0HQ,,,,,non-homologous end joining,mechanism of DNA end-joining repair in which DNA ends are joined in a homology-independent manner,,NHEJ repair of DNA breaks in genome editing workflows can result in indel formation.,,,,
http://webprotege.stanford.edu/RCGwa1fl6GEgzC06l68hNwV,ZFN recognition helix,,seven residue positions within a zinc finger that are most directly responsible for its DNA binding preference,false,,,http://webprotege.stanford.edu/R7XppaHUTo1MnKzZFHCqU6I,,,,,,seven residue positions within a zinc finger that are most directly responsible for its DNA binding preference,,"The seven residues comprise the first six residues of the alpha helix, along with the residue immediately preceding the N-terminal of the helix. They are typically referred to as positions +1 to +6 (within the alpha helix) and position (−1) (immediately preceding the helix).",,,,
http://webprotege.stanford.edu/R92zRapIYUCrO4dJgiuz4sI,HDR,homology-directed repair,mechanism of recombinational DNA repair where repair is templated by a polynucleotide with regions corresponding to sequences flanking the target,false,,,http://webprotege.stanford.edu/RjAHARFJxxBxFl0vcXU0HQ,,,,,homology-directed repair,mechanism of recombinational DNA repair where repair is templated by a polynucleotide with regions corresponding to sequences flanking the target,Single-stranded DNA oligonucleotide templated HDR.,Repair templates can be exogenously introduced to achieve sequence changes in genome editing approaches.,,,,
http://webprotege.stanford.edu/R7XppaHUTo1MnKzZFHCqU6I,ZFN specific,,,false,,,http://webprotege.stanford.edu/RDyioNYXAgMHUox9J3W3veJ,,,,,,,,,,,,
http://webprotege.stanford.edu/R9sP7XzKAY6GL146IWfcRK5,ZFN,zinc finger nuclease,chimeric protein consisting of an array of zinc fingers linked to a DNA cleavage domain,false,,,http://webprotege.stanford.edu/R7XppaHUTo1MnKzZFHCqU6I,,,,,zinc finger nuclease,chimeric protein consisting of an array of zinc fingers linked to a DNA cleavage domain,,FokI is prevalently used as the DNA cleavage domain bound to a zinc finger.|Binding of two ZFNs to a pair of appropriately spaced DNA target sites enables nuclease domain dimerization and DNA cleavage between the targets.,,,,
http://webprotege.stanford.edu/R8ANIoT1lyTBDgdQbb9AZfu,edit,"DNA, RNA or epigenome edit|RNA edit|epigenome edit|DNA edit",modification to nucleic acid sequence resulting from the application of genome editing components,false,,,http://webprotege.stanford.edu/RjAHARFJxxBxFl0vcXU0HQ,,,,,"DNA, RNA or epigenome edit|RNA edit|epigenome edit|DNA edit",modification to nucleic acid sequence resulting from the application of genome editing components,"Insertion, deletion, substitution, deamination, methylation, demethylation.",Genome editing components can include a nuclease and repair template,,,,
http://webprotege.stanford.edu/R7ntN3MoEN2EzJxKwgm4XlX,unintended edit,,modification to nucleic acid at the target that is not the designed change or at an off-target resulting from the application of genome editing components”,false,,,http://webprotege.stanford.edu/RjAHARFJxxBxFl0vcXU0HQ,,,,,,modification to nucleic acid at the target that is not the designed change or at an off-target resulting from the application of genome editing components”,,Genome editing components can include a nuclease and repair template,,,,
